{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:28:48Z","timestamp":1762100928068,"version":"3.40.3"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>CD1d is a nonclassical Ag-presenting molecule that presents glycolipid Ags to NKT cells that are involved in immune defense and tumor rejection. It also plays a role in immunoregulatory functions in the epidermis. The mechanisms controlling the expression of CD1d are not well understood. Therefore, we cloned the CD1d gene promoter and characterized its activities in primary human keratinocytes and other cell lines of epithelial origin. We found that a CCAAT box in the CD1d promoter is required for its expression in keratinocytes. We show here that transcription factor C\/EBP-\u03b2 binds to the CCAAT box in the CD1d promoter in vitro and in vivo. Consistent with these observations, deletion of the gene encoding for C\/EBP-\u03b2 caused a loss of CD1d expression. The in vivo regulation of CD1d has significant implications for the pathologic mechanisms of certain immunologic skin diseases in which NKT cells play a role, such as allergic contact dermatitis and psoriasis. Together, these data show a central role for C\/EBP-\u03b2 in regulating CD1d transcription.<\/jats:p>","DOI":"10.4049\/jimmunol.0900057","type":"journal-article","created":{"date-parts":[[2009,7,11]],"date-time":"2009-07-11T01:40:49Z","timestamp":1247276449000},"page":"1657-1666","source":"Crossref","is-referenced-by-count":11,"title":["A Central Role for Transcription Factor C\/EBP-\u03b2 in Regulating CD1d Gene Expression in Human Keratinocytes"],"prefix":"10.1093","volume":"183","author":[{"given":"Hashmat","family":"Sikder","sequence":"first","affiliation":[{"name":"Department of Dermatology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Balato","sequence":"additional","affiliation":[{"name":"Department of Dermatology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre","family":"Chapoval","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology-Head and Neck Surgery"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Fishelevich","sequence":"additional","affiliation":[{"name":"Department of Dermatology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Padmaja","family":"Gade","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ishwar S","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Maryland School of Medicine , Baltimore, MD 21201"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhananjaya V","family":"Kalvakolanu","sequence":"additional","affiliation":[{"name":"National Cancer Institute , Frederick, MD 21702"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Dermatology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony A","family":"Gaspari","sequence":"additional","affiliation":[{"name":"Department of Dermatology"},{"name":"Department of Microbiology and Immunology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,8,1]]},"reference":[{"key":"2025032709085477400_R1","first-page":"377","article-title":"The role of KCs in the pathophysiology of contact dermatitis.","volume-title":"Immunol. 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Immunol.","year":"2006"},{"key":"2025032709085477400_R6","first-page":"3709","article-title":"CD1 and the expanding universe of T cell antigens.","volume-title":"J. Immunol.","year":"1995"},{"key":"2025032709085477400_R7","first-page":"109","article-title":"Requirements for CD1d recognition by human invariant Va24+CD4\u2212CD8\u2212 T cells.","volume-title":"J. Exp. Med.","year":"1997"},{"key":"2025032709085477400_R8","first-page":"561","article-title":"Tissue distribution of the non-polymorphic major histocompatibility complex class I-like molecule CD1d.","volume-title":"Immunology","year":"1993"},{"key":"2025032709085477400_R9","first-page":"40369","article-title":"An N-linked glycan modulates the interaction between the CD1d heavy chain and \u03b22-microglobulin.","volume-title":"J. Biol. Chem.","year":"2006"},{"key":"2025032709085477400_R10","first-page":"1216","article-title":"Mouse CD1 is mainly expressed on hemopoietic derived cells.","volume-title":"J. 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Immunol.","year":"1991"},{"key":"2025032709085477400_R15","first-page":"C276","article-title":"IFN-\u03b3 modulates CD1d surface expression intestinal epithelia.","volume-title":"Am. J. Physiol.","year":"1996"},{"key":"2025032709085477400_R16","first-page":"37","article-title":"CD1d structure and regulation on human thymocytes, peripheral blood T-cells, B-cells and monocytes.","volume-title":"Immunology","year":"2000"},{"key":"2025032709085477400_R17","first-page":"1460","article-title":"Human natural killer T cells infiltrate into the skin at elicitation sites of allergic contact dermatitis.","volume-title":"J. Invest. Dermatol.","year":"2008"},{"key":"2025032709085477400_R18","first-page":"269","article-title":"iNKT cells in allergic disease.","volume-title":"Curr. Top. Microbiol. 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