{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T04:37:35Z","timestamp":1769661455302,"version":"3.49.0"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Follicular dendritic cells (FDCs) are located in the lymphoid follicles of secondary lymphoid tissues and play a pivotal role in the selection of memory B lymphocytes within the germinal center, a major site for HIV-1 infection. Germinal centers are composed of highly activated B cells, macrophages, CD4+T cells, and FDCs. However, the physiological role of FDCs in HIV-1 replication remains largely unknown. We demonstrate in our current study that FDCs can efficiently activate HIV-1 replication in latently infected monocytic cells via an intercellular communication network mediated by the P-selectin\/P-selectin glycoprotein ligand 1 (PSGL-1) interaction. Upon coculture with FDCs, HIV-1 replication was significantly induced in infected monocytic cell lines, primary monocytes, or macrophages. These cocultures were found to synergistically induce the expression of P-selectin in FDCs via NF-\u03baB activation and its cognate receptor PSGL-1 in HIV-1-infected cells. Consistent with this observation, we find that this response is significantly blocked by antagonistic Abs against PSGL-1 and almost completely inhibited by PSGL-1 small interfering RNA. Moreover, a selective inhibitor for Syk, which is a downstream effector of PSGL-1, blocked HIV-1 replication in our cultures. We have thus elucidated a novel regulatory mechanism in which FDCs are a potent positive bystander that facilitates HIV-1 replication in adjacent infected monocytic cells via a juxtacrine signaling mechanism.<\/jats:p>","DOI":"10.4049\/jimmunol.0900371","type":"journal-article","created":{"date-parts":[[2009,6,19]],"date-time":"2009-06-19T17:04:08Z","timestamp":1245431048000},"page":"524-532","source":"Crossref","is-referenced-by-count":10,"title":["Follicular Dendritic Cells Activate HIV-1 Replication in Monocytes\/Macrophages through a Juxtacrine Mechanism Mediated by P-Selectin Glycoprotein Ligand 1"],"prefix":"10.1093","volume":"183","author":[{"given":"Kenji","family":"Ohba","sequence":"first","affiliation":[{"name":"AIDS Research Center, National Institute of Infectious Diseases , Toyama, Shinjyuku-ku, Tokyo,","place":["Japan"]},{"name":"Department of Molecular Virology, Graduate School of Medicine, Tokyo Medical and Dental University , Yushima, Bunkyo-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihide","family":"Ryo","sequence":"additional","affiliation":[{"name":"AIDS Research Center, National Institute of Infectious Diseases , Toyama, Shinjyuku-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md Zahidunnabi","family":"Dewan","sequence":"additional","affiliation":[{"name":"AIDS Research Center, National Institute of Infectious Diseases , Toyama, Shinjyuku-ku, Tokyo,","place":["Japan"]},{"name":"Department of Molecular Virology, Graduate School of Medicine, Tokyo Medical and Dental University , Yushima, Bunkyo-ku, Tokyo,","place":["Japan"]},{"name":"Department of Pathology, New York University School of Medicine , New York, NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayuko","family":"Nishi","sequence":"additional","affiliation":[{"name":"AIDS Research Center, National Institute of Infectious Diseases , Toyama, Shinjyuku-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Juntendo University School of Medicine , Hongo, Bunkyo-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohua","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Graduate School of Medicine, Tokyo Medical and Dental University , Yushima, Bunkyo-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Inagaki","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Graduate School of Medicine, Tokyo Medical and Dental University , Yushima, Bunkyo-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoji","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Department of Pathology, Graduate School of Medicine, Yokohama City University , Fukuura, Kanazawa-ku, Kanagawa,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuetsu","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Immunology, Graduate School of Medicine, University of the Ryukyus , Uehara, Okinawa,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology, Nagoya City University Graduate School of Medical Sciences , Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Terashima","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology, Graduate School of Medicine, Tokyo Medical and Dental University , Yushima, Bunkyo-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"AIDS Research Center, National Institute of Infectious Diseases , Toyama, Shinjyuku-ku, Tokyo,","place":["Japan"]},{"name":"Department of Molecular Virology, Graduate School of Medicine, Tokyo Medical and Dental University , Yushima, Bunkyo-ku, Tokyo,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,7,1]]},"reference":[{"key":"2026012811274559100_R1","first-page":"853","article-title":"HIV-1 pathogenesis.","volume-title":"Nat. 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