{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:49:45Z","timestamp":1779115785523,"version":"3.51.4"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2009,12,30]],"date-time":"2009-12-30T00:00:00Z","timestamp":1262131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54 AI065357-01"],"award-info":[{"award-number":["U54 AI065357-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Burkholderia mallei is a Gram-negative bacterial pathogen of domestic equidae and humans that can cause severe, rapidly life-threatening pneumonic infections. Little is known regarding the role of chemokines and early cellular immune responses in protective immunity to pulmonary infection with B. mallei. Although the role of MCP-1 in Gram-positive bacterial infections has been previously investigated, the role of MCP-1 in immunity to acute pneumonia caused by Gram-negative bacteria, such as B. mallei, has not been assessed. In a mouse model of pneumonic B. mallei infection, we found that both MCP-1\u2212\/\u2212 mice and CCR2\u2212\/\u2212 mice were extremely susceptible to pulmonary infection with B. mallei, compared with wild-type (WT) C57Bl\/6 mice. Bacterial burden and organ lesions were significantly increased in CCR2\u2212\/\u2212 mice, compared with WT animals, following B. mallei challenge. Monocyte and dendritic cell recruitment into the lungs of CCR2\u2212\/\u2212 mice was significantly reduced in comparison with that in WT mice following B. mallei infection, whereas neutrophil recruitment was actually increased. Depletion of monocytes and macrophages prior to infection also greatly raised the susceptibility of WT mice to infection. Production of IL-12 and IFN-\u03b3 in the lungs after B. mallei infection was significantly impaired in both MCP-1\u2212\/\u2212 and CCR2\u2212\/\u2212 mice, whereas treatment of CCR2\u2212\/\u2212 mice with rIFN-\u03b3 restored protection against lethal challenge with B. mallei. Thus, we conclude that MCP-1 plays a key role in regulating cellular immunity and IFN-\u03b3 production following pneumonic infection with B. mallei and therefore may also figure importantly in other Gram-negative pneumonias.<\/jats:p>","DOI":"10.4049\/jimmunol.0900411","type":"journal-article","created":{"date-parts":[[2009,12,30]],"date-time":"2009-12-30T22:01:23Z","timestamp":1262210483000},"page":"1445-1454","source":"Crossref","is-referenced-by-count":25,"title":["Critical Protective Role for MCP-1 in Pneumonic\n                    <i>Burkholderia mallei<\/i>\n                    Infection"],"prefix":"10.1093","volume":"184","author":[{"given":"Andrew","family":"Goodyear","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abby","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Troyer","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helle","family":"Bielefeldt-Ohmann","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Dow","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology"},{"name":"Department of Clinical Sciences, Colorado State University , Ft. 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