{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:39:00Z","timestamp":1790008740824,"version":"4.0.1"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The human positive regulatory domain I-binding factor 1 (PRDI-BF1) and its murine homolog Blimp-1 promote differentiation of mature B cells into Ab-secreting plasma cells. In contrast, ectopic expression of PRDI-BF1 in lymphoma cells can lead to inhibition of proliferation or apoptosis. However, little is currently known about the regulation of PRDM1, the gene encoding PRDI-BF1. This report establishes that in lymphoma cells stimulation through the BCR rapidly induces endogenous PRDM1 at the level of transcription with minor changes in mRNA stability. The induced PRDM1-encoded protein localizes to its target genes in vivo and suppresses their expression. In vivo genomic footprinting of the PRDM1 promoter in unstimulated lymphoma and myeloma cells reveals multiple common in vivo occupied elements throughout the promoter. Further functional and structural analysis of the promoter reveals that the promoter is preloaded and poised for activation in the B cell lines. The transcription factor PU.1 is shown to be required for the BCR-induced expression of PRDM1 in lymphoma cells and in PU.1-positive myeloma cells. Activation of PRDM1 is associated with loss of the corepressor transducin-like enhancer of split 4 from the PU.1 complex. These findings indicate that PRDM1 is poised for activation in lymphoma cells and therefore may be a potential therapeutic target to inhibit lymphoma cell proliferation and survival.<\/jats:p>","DOI":"10.4049\/jimmunol.0901120","type":"journal-article","created":{"date-parts":[[2009,10,14]],"date-time":"2009-10-14T21:31:53Z","timestamp":1255555913000},"page":"5778-5787","source":"Crossref","is-referenced-by-count":18,"title":["PU.1 Regulates Positive Regulatory Domain I-Binding Factor 1\/Blimp-1 Transcription in Lymphoma Cells"],"prefix":"10.1093","volume":"183","author":[{"given":"Shruti","family":"Desai","sequence":"first","affiliation":[{"name":"H. Lee Moffitt Cancer Center and Research Institute , Tampa, FL 33612"},{"name":"Cancer Biology Ph.D. Program"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia C E","family":"Bolick","sequence":"additional","affiliation":[{"name":"H. Lee Moffitt Cancer Center and Research Institute , Tampa, FL 33612"},{"name":"Department of Molecular Medicine, University of South Florida , Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Maurin","sequence":"additional","affiliation":[{"name":"H. Lee Moffitt Cancer Center and Research Institute , Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth L","family":"Wright","sequence":"additional","affiliation":[{"name":"H. Lee Moffitt Cancer Center and Research Institute , Tampa, FL 33612"},{"name":"Cancer Biology Ph.D. Program"},{"name":"Department of Oncologic Sciences"},{"name":"Department of Molecular Medicine, University of South Florida , Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,11,1]]},"reference":[{"key":"2026012811155252600_R1","first-page":"868","article-title":"Identification and characterization of a novel repressor of \u03b2-interferon gene expression.","volume-title":"Genes Dev.","year":"1991"},{"key":"2026012811155252600_R2","first-page":"297","article-title":"Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells.","volume-title":"Cell","year":"1994"},{"key":"2026012811155252600_R3","first-page":"607","article-title":"Blimp-1 is required for the formation of immunoglobulin secreting plasma cells and pre-plasma memory B cells.","volume-title":"Immunity","year":"2003"},{"key":"2026012811155252600_R4","first-page":"526","article-title":"BLIMP-I mediates extinction of major histocompatibility class II transactivator expression in plasma cells.","volume-title":"Nat. Immunol.","year":"2000"},{"key":"2026012811155252600_R5","first-page":"15264","article-title":"Positive regulatory domain I binding factor 1 silences class II transactivator expression in multiple myeloma cells.","volume-title":"J. Biol. Chem.","year":"2001"},{"key":"2026012811155252600_R6","first-page":"596","article-title":"Repression of c-myc transcription by Blimp-1, an inducer of terminal B cell differentiation.","volume-title":"Science","year":"1997"},{"key":"2026012811155252600_R7","first-page":"4771","article-title":"Blimp-1-dependent repression of Pax-5 is required for differentiation of B cells to immunoglobulin M-secreting plasma cells.","volume-title":"Mol. Cell Biol.","year":"2002"},{"key":"2026012811155252600_R8","first-page":"51","article-title":"Blimp-1 orchestrates plasma cell differentiation by extinguishing the mature B cell gene expression program.","volume-title":"Immunity","year":"2002"},{"key":"2026012811155252600_R9","first-page":"1572","article-title":"BLIMP-1 is a target of cellular stress and downstream of the unfolded protein response.","volume-title":"Eur. J. Immunol.","year":"2006"},{"key":"2026012811155252600_R10","first-page":"1405","article-title":"Anti-immunoglobulins induce death by apoptosis in WEHI-231 B lymphoma cells.","volume-title":"Eur. J. Immunol.","year":"1990"},{"key":"2026012811155252600_R11","first-page":"1618","article-title":"Role of phosphatidylinositol 3-kinase in anti-IgM- and anti-IgD-induced apoptosis in B cell lymphomas.","volume-title":"J. Immunol.","year":"2001"},{"key":"2026012811155252600_R12","first-page":"1685","article-title":"Anti-immunoglobulin antibodies induce apoptosis in immature B cell lymphomas.","volume-title":"Eur. J. Immunol.","year":"1990"},{"key":"2026012811155252600_R13","first-page":"18875","article-title":"Anti-IgM-mediated regulation of c-myc and its possible relationship to apoptosis.","volume-title":"J. Biol. Chem.","year":"1996"},{"key":"2026012811155252600_R14","first-page":"1365","article-title":"CD38 expression distinguishes two groups of B-cell chronic lymphocytic leukemias with different responses to anti-IgM antibodies and propensity to apoptosis.","volume-title":"Blood","year":"1996"},{"key":"2026012811155252600_R15","first-page":"923","article-title":"Activation of terminal B cell differentiation by inhibition of histone deacetylation.","volume-title":"Mol. Immunol.","year":"2003"},{"key":"2026012811155252600_R16","first-page":"2988","article-title":"Reversal of Blimp-1-mediated apoptosis by A1, a member of the Bcl-2 family.","volume-title":"Eur. J. Immunol.","year":"1999"},{"key":"2026012811155252600_R17","first-page":"515","article-title":"Differential effect of B lymphocyte-induced maturation protein (Blimp-1) expression on cell fate during B cell development.","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026012811155252600_R18","first-page":"467","article-title":"PRDM1\/BLIMP-1 expression in multiple B and T-cell lymphoma.","volume-title":"Haematologica","year":"2006"},{"key":"2026012811155252600_R19","first-page":"311","article-title":"Inactivation of the PRDM1\/BLIMP1 gene in diffuse large B cell lymphoma.","volume-title":"J. Exp. Med.","year":"2006"},{"key":"2026012811155252600_R20","first-page":"4090","article-title":"Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas.","volume-title":"Blood","year":"2006"},{"key":"2026012811155252600_R21","first-page":"3125","article-title":"Identification of a functionally impaired positive regulatory domain I binding factor 1 transcription repressor in myeloma cell lines.","volume-title":"J. Immunol.","year":"2003"},{"key":"2026012811155252600_R22","first-page":"4629","article-title":"Altered mRNA expression of Pax5 and Blimp-1 in B cells in multiple myeloma.","volume-title":"Blood","year":"2002"},{"key":"2026012811155252600_R23","first-page":"1265","article-title":"Fas ligation induces apoptosis of CD40-activated human B lymphocytes.","volume-title":"J. Exp. Med.","year":"1995"},{"key":"2026012811155252600_R24","first-page":"2320","article-title":"CpG DNA induces a class II transactivator-independent increase in class II MHC by stabilizing class II MHC mRNA in B lymphocytes.","volume-title":"J. Immunol.","year":"2003"},{"key":"2026012811155252600_R25","first-page":"7219","article-title":"Physical isolation of nascent RNA chains transcribed by RNA polymerase II: evidence for cotranscriptional splicing.","volume-title":"Mol. Cell Biol.","year":"1994"},{"key":"2026012811155252600_R26","first-page":"73","article-title":"Strategies and tools for whole-genome alignments.","volume-title":"Genome Res.","year":"2003"},{"key":"2026012811155252600_R27","first-page":"32342","article-title":"A novel element and a TEF-2-like element activate the major histocompatibility complex class II transactivator in B-lymphocytes.","volume-title":"J. Biol. Chem.","year":"1999"},{"key":"2026012811155252600_R28","first-page":"1277","article-title":"In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1.","volume-title":"Genes Dev.","year":"1990"},{"key":"2026012811155252600_R29","first-page":"1475","article-title":"Accurate transcription initiation by RNA pol II in a soluble extract from isolated mammalian nuclei.","volume-title":"Nucleic Acids Res.","year":"1983"},{"key":"2026012811155252600_R30","first-page":"596","article-title":"Repression of c-myc Transcription by Blimp-1, an inducer of terminal B cell differentiation.","volume-title":"Science","year":"1997"},{"key":"2026012811155252600_R31","first-page":"7046","article-title":"Extreme instability of myc mRNA in normal and transformed human cells.","volume-title":"Proc. Natl. Acad. Sci. 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Immunol.","year":"2004"},{"key":"2026012811155252600_R36","first-page":"2316","article-title":"Transcription of PRDM1, the master regulator for plasma cell differentiation, depends on an SP1\/SP3\/EGR-1 GC-box.","volume-title":"Eur. J. Immunol.","year":"2008"},{"key":"2026012811155252600_R37","first-page":"1922","article-title":"Repression of AP-1 function: a mechanism for the regulation of Blimp-1 expression and B lymphocyte differentiation by the B cell lymphoma-6 protooncogene.","volume-title":"J. Immunol.","year":"2002"},{"key":"2026012811155252600_R38","first-page":"291","article-title":"Corecruitment of the Grg4 repressor by PU.1 is critical for Pax5-mediated repression of B-cell-specific genes.","volume-title":"EMBO Rep.","year":"2004"},{"key":"2026012811155252600_R39","first-page":"1495","article-title":"Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP.","volume-title":"Oncogene","year":"1999"},{"key":"2026012811155252600_R40","first-page":"230","article-title":"Regulation of plasma-cell development.","volume-title":"Nat. Rev. Immunol.","year":"2005"},{"key":"2026012811155252600_R41","first-page":"1301","article-title":"Targeting positive regulatory domain I-binding factor 1 and X box-binding protein 1 transcription factors by multiple myeloma-reactive CTL.","volume-title":"J. Immunol.","year":"2005"},{"key":"2026012811155252600_R42","first-page":"7703","article-title":"A role for c-fos\/activator protein 1 in B lymphocyte terminal differentiation.","volume-title":"J. Immunol.","year":"2005"},{"key":"2026012811155252600_R43","first-page":"1573","article-title":"Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages.","volume-title":"Science","year":"1994"},{"key":"2026012811155252600_R44","first-page":"5328","article-title":"Down-regulation of PU.1 by methylation of distal regulatory elements and the promoter is required for myeloma cell growth.","volume-title":"Cancer Res.","year":"2007"},{"key":"2026012811155252600_R45","first-page":"8688","article-title":"Direct association between PU.1 and MeCP2 that recruits mSin3A-HDAC complex for PU.1-mediated transcriptional repression.","volume-title":"Oncogene","year":"2003"},{"key":"2026012811155252600_R46","first-page":"199","article-title":"BCL-6 represses genes that function in lymphocyte differentiation, inflammation, and cell cycle control.","volume-title":"Immunity","year":"2000"},{"key":"2026012811155252600_R47","first-page":"38226","article-title":"Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells.","volume-title":"J. Biol. Chem.","year":"2006"},{"key":"2026012811155252600_R48","first-page":"6153","article-title":"Displacement of an E-box-binding repressor by basic helix-loop-helix proteins: implications for B-cell specificity of the immunoglobulin heavy-chain enhancer.","volume-title":"Mol. 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