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Because of rErbb2 expression in the thymus and its overexpression in the mammary gland, CD8+ T cell clones reacting at high avidity with dominant rErbb2 epitopes are deleted in these mice. In BALB-neuT mice with diffuse and invasive in situ lesions and almost palpable carcinomas, a temporary regulatory T cells depletion combined with anti-rErbb2 vaccine markedly enhanced the anti-rErbb2 Ab response and allowed the expansion of latent pools of low-avidity CD8+ T cells bearing TCRs repertoire reacting with the rErbb2 dominant peptide. This combination of a higher Ab response and activation of a low-avidity cytotoxic response persistently blocked tumor progression at stages in which the vaccine alone was ineffective. However, when diffuse and invasive microscopic cancers become almost palpable, this combination was no longer able to secure a significant extension of mice survival.<\/jats:p>","DOI":"10.4049\/jimmunol.0901215","type":"journal-article","created":{"date-parts":[[2010,4,30]],"date-time":"2010-04-30T22:16:05Z","timestamp":1272665765000},"page":"6124-6132","source":"Crossref","is-referenced-by-count":24,"title":["Erbb2 DNA Vaccine Combined with Regulatory T Cell Deletion Enhances Antibody Response and Reveals Latent Low-Avidity T Cells: Potential and Limits of Its Therapeutic Efficacy"],"prefix":"10.1093","volume":"184","author":[{"given":"Simona","family":"Rolla","sequence":"first","affiliation":[{"name":"Molecular Biotechnology Center, Department of Clinical and Biological Sciences, University of Turin , Turin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Ria","sequence":"additional","affiliation":[{"name":"Institute of General Pathology, Catholic University Sacro Cuore , Rome"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Occhipinti","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology Center, Department of Clinical and Biological Sciences, University of Turin , Turin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriele","family":"Di Sante","sequence":"additional","affiliation":[{"name":"Institute of General Pathology, Catholic University Sacro Cuore , Rome"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Iezzi","sequence":"additional","affiliation":[{"name":"Aging Research Center (Centro Studi Invecchiamento), G. d\u2019Annunzio University Foundation , Chieti,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michela","family":"Spadaro","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology Center, Department of Clinical and Biological Sciences, University of Turin , Turin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Nicol\u00f2","sequence":"additional","affiliation":[{"name":"Institute of General Pathology, Catholic University Sacro Cuore , Rome"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Ambrosino","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology Center, Department of Clinical and Biological Sciences, University of Turin , Turin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene Fiore","family":"Merighi","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology Center, Department of Clinical and Biological Sciences, University of Turin , Turin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piero","family":"Musiani","sequence":"additional","affiliation":[{"name":"Aging Research Center (Centro Studi Invecchiamento), G. d\u2019Annunzio University Foundation , Chieti,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Forni","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology Center, Department of Clinical and Biological Sciences, University of Turin , Turin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Cavallo","sequence":"additional","affiliation":[{"name":"Molecular Biotechnology Center, Department of Clinical and Biological Sciences, University of Turin , Turin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,6,1]]},"reference":[{"key":"2026012806310152700_r1","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/nrc1815","article-title":"Vaccines for tumour prevention.","volume":"6","author":"Lollini","year":"2006","journal-title":"Nat. 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