{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:56:58Z","timestamp":1790308618572,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dendritic cells (DC) licensed by the interaction between pathogen products and pattern recognition receptors can activate naive T cells to undergo Ag-dependent proliferation and cytokine production. In contrast, DC induced to mature by trans-acting inflammatory stimuli are believed to only be capable of supporting Ag-dependent proliferative responses. In this study, we show that uninfected DC matured as a consequence of Leishmania-induced inflammation induce CD8+ T cells to proliferate in the absence of their cognate Ag. We separated splenic DC from Leishmania donovani-infected mice into those that contained parasites and had been activated to induce IL-12p40, from those that had undergone only partial maturation, measured by increased CD86 expression in the absence of IL-12p40 induction. We then showed that these partially matured DC could induce exogenous peptide-independent proliferation of OT-I and F5 CD8+ TCR transgenic T cells, as well as polyclonal CD8+ T cells. Proliferation of OT-I cells was significantly inhibited in vitro and in vivo by anti-CD86 mAb but not by anti-CD80 mAb and could also be inhibited by cyclosporine A. Proliferating OT-I cells did not produce IFN-\u03b3, even when re-exposed to mature DC. However, these primed OT-I cells subsequently produced effector cytokines, not just on exposure to their cognate peptide but, more importantly, to weak exogenous TCR agonists that otherwise failed to induce IFN-\u03b3. We further showed that OT-I cells undergoing locally driven proliferation to another pathogen, Streptococcus pneumoniae, rapidly seeded other lymphoid tissues, suggesting that CD8+ T cells primed in this way may play a role in rapidly countering pathogen dissemination.<\/jats:p>","DOI":"10.4049\/jimmunol.0901330","type":"journal-article","created":{"date-parts":[[2009,11,16]],"date-time":"2009-11-16T21:55:14Z","timestamp":1258408514000},"page":"7095-7103","source":"Crossref","is-referenced-by-count":21,"title":["Dendritic Cells Matured by Inflammation Induce CD86-Dependent Priming of Naive CD8+ T Cells in the Absence of Their Cognate Peptide Antigen"],"prefix":"10.1093","volume":"183","author":[{"given":"Asher","family":"Maroof","sequence":"first","affiliation":[{"name":"Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York , Wentworth Way, York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynette","family":"Beattie","sequence":"additional","affiliation":[{"name":"Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York , Wentworth Way, York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alun","family":"Kirby","sequence":"additional","affiliation":[{"name":"Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York , Wentworth Way, York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Coles","sequence":"additional","affiliation":[{"name":"Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York , Wentworth Way, York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M","family":"Kaye","sequence":"additional","affiliation":[{"name":"Centre for Immunology and Infection, Hull York Medical School and Department of Biology, University of York , Wentworth Way, York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,12,1]]},"reference":[{"key":"2026012811271638900_R1","first-page":"419","article-title":"Taking dendritic cells into medicine.","volume-title":"Nature","year":"2007"},{"key":"2026012811271638900_R2","first-page":"118","article-title":"Dendritic cell subsets in health and disease.","volume-title":"Immunol. 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