{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:48:34Z","timestamp":1789876114912,"version":"4.0.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T00:00:00Z","timestamp":1263945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Although studies suggest that NKT cell (NKT) activation modulates the function of dendritic cells (DCs) in inducing T cell responses, it is unknown whether this modulating effect is biased to a DC subset. We previously reported that NKT activation could modulate DC function in inducing protective T cell immunity to Chlamydia pneumoniae, an intracellular bacterial infection. In this study, we investigated the effect of NKT activation on DC subsets, using multiple approaches, including gene knockout mice, \u03b1- galactosylceramide stimulation, adoptive transfer of invariant NKT (iNKT), and functional analysis of DC subsets in both in vitro and in vivo settings. We found a preferential modulating effect of iNKTs on the CD8\u03b1+ DC subset. Specifically, we found that iNKT-deficient mice, compared with wild-type (WT) mice, showed reduced CD8\u03b1+ DC expansion with lower CD40 expression and IL-12 production, whereas enhancing iNKT activation in WT mice or adoptive transfer of iNKTs to J\u03b118\u2212\/\u2212 mice resulted in increased function of CD8\u03b1+ DCs in inducing type 1 immune responses. Further, DC-iNKT coculture experiments showed a direct CD40L-dependent enhancing effect of iNKTs on IL-12p70 production by CD8\u03b1+ DCs. More importantly, CD8\u03b1+ DCs from J\u03b118\u2212\/\u2212 mice, compared with those from WT mice, showed significantly reduced ability to activate IFN-\u03b3\u2013producing T cells in vitro and to induce type 1 immunity and protection in vivo. Moreover, a similar CD8\u03b1+ DC subset alteration was found in the J\u03b118\u2212\/\u2212 mice following Leishmania major infection. Our data provide the first direct evidence that iNKTs preferentially promote the functional development of a subset of DC to generate protective immunity against infections.<\/jats:p>","DOI":"10.4049\/jimmunol.0901348","type":"journal-article","created":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T21:55:00Z","timestamp":1264024500000},"page":"2095-2106","source":"Crossref","is-referenced-by-count":46,"title":["Invariant NKT Cells Preferentially Modulate the Function of CD8\u03b1+ Dendritic Cell Subset in Inducing Type 1 Immunity against Infection"],"prefix":"10.1093","volume":"184","author":[{"given":"Antony George","family":"Joyee","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jude","family":"Uzonna","sequence":"additional","affiliation":[{"name":"Department of Immunology, Laboratory for Infection and Immunity, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology"},{"name":"Department of Immunology, Laboratory for Infection and Immunity, Faculty of Medicine, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,1,20]]},"reference":[{"key":"2026012806272363500_r1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/32588","article-title":"Dendritic cells and the control of immunity.","volume":"392","author":"Banchereau","year":"1998","journal-title":"Nature"},{"key":"2026012806272363500_r2","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1073\/pnas.96.3.1036","article-title":"Distinct dendritic cell subsets differentially regulate the class of immune response in vivo.","volume":"96","author":"Pulendran","year":"1999","journal-title":"Proc. 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