{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:57:39Z","timestamp":1791115059785,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Langerhans cells (LCs) are dendritic cells (DCs) localized in stratified epithelia, such as those overlaying skin, buccal mucosa, and vagina. The contribution of LCs to the promotion or control of immunity initiated at epithelial sites remains debated. We report in this paper that an immunogen comprising OVA linked to the B subunit of cholera toxin, used as delivery vector, was efficient to generate CTLs after vaginal immunization. Using Lang-EGFP mice, we evaluated the contribution of distinct DC subsets to the generation of CD4 and CD8 T cell responses. We demonstrate that the vaginal epithelium, unlike the skin epidermis, includes a minor population of LCs and a major subset of langerin\u2212 DCs. Intravaginally administered Ag is taken up by LCs and langerin\u2212 DCs and carried up to draining lymph nodes, where both subsets prime CD8 T cells, unlike blood-derived DCs, although with distinct capabilities. LCs prime CD8 T cells with a cytokine profile dominated by IL-17, whereas Lang\u2212 DCs induce IFN-\u03b3\u2013producing T cells. Using Lang-DTR-EGFP mice to ensure a transient ablation of LCs, we found that these cells not only are dispensable for the generation of genital CTL responses but also downregulate these responses, by a mechanism that may involve IL-10 and IL-17 cytokines. This finding has implications for the development of mucosal vaccines and immunotherapeutic strategies designed for the targeting of DCs.<\/jats:p>","DOI":"10.4049\/jimmunol.0901695","type":"journal-article","created":{"date-parts":[[2010,3,29]],"date-time":"2010-03-29T23:34:10Z","timestamp":1269905650000},"page":"4842-4851","source":"Crossref","is-referenced-by-count":39,"title":["Langerhans Cells Prime IL-17\u2013Producing T Cells and Dampen Genital Cytotoxic Responses following Mucosal Immunization"],"prefix":"10.1093","volume":"184","author":[{"given":"Catherine","family":"Hervouet","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U634, Facult\u00e9 de M\u00e9decine Pasteur"},{"name":"University of Nice-Sophia Antipolis , Nice"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmelo","family":"Luci","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U634, Facult\u00e9 de M\u00e9decine Pasteur"},{"name":"University of Nice-Sophia Antipolis , Nice"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Rol","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U634, Facult\u00e9 de M\u00e9decine Pasteur"},{"name":"University of Nice-Sophia Antipolis , Nice"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9borah","family":"Rousseau","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U634, Facult\u00e9 de M\u00e9decine Pasteur"},{"name":"University of Nice-Sophia Antipolis , Nice"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Kissenpfennig","sequence":"additional","affiliation":[{"name":"Centre d'Immunologie de Marseille-Luminy, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale-Centre National de la Recherche Scientifique, Universit\u00e9 de la M\u00e9diterran\u00e9e , Marseille,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Malissen","sequence":"additional","affiliation":[{"name":"Centre d'Immunologie de Marseille-Luminy, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale-Centre National de la Recherche Scientifique, Universit\u00e9 de la M\u00e9diterran\u00e9e , Marseille,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecil","family":"Czerkinsky","sequence":"additional","affiliation":[{"name":"International Vaccine Institute , Seoul,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Anju\u00e8re","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U634, Facult\u00e9 de M\u00e9decine Pasteur"},{"name":"University of Nice-Sophia Antipolis , Nice"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,5,1]]},"reference":[{"key":"2026012808075991900_r1","first-page":"p. 1631","article-title":"Mucosal immunity in the human female reproductive tract","volume-title":"Mucosal Immunology","author":"Kuttey","year":"2004","edition":"3rd Ed"},{"key":"2026012808075991900_r2","first-page":"p. 1614","article-title":"Female genital tract infections and immunity in animal models.","volume-title":"Mucosal Immunology","author":"Parr","year":"2004","edition":"3rd Ed"},{"key":"2026012808075991900_r3","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/79703","article-title":"AIDS in Africa: a disaster no longer waiting to happen.","volume":"1","author":"Kaul","year":"2000","journal-title":"Nat. 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Lett."},{"key":"2026012808075991900_r6","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1128\/IAI.66.2.514-520.1998","article-title":"Antibodies and antibody-secreting cells in the female genital tract after vaginal or intranasal immunization with cholera toxin B subunit or conjugates.","volume":"66","author":"Johansson","year":"1998","journal-title":"Infect. Immun."},{"key":"2026012808075991900_r7","doi-asserted-by":"crossref","first-page":"5889","DOI":"10.1128\/IAI.66.12.5889-5896.1998","article-title":"Specific-antibody-secreting cells in the rectums and genital tracts of nonhuman primates following vaccination.","volume":"66","author":"Eriksson","year":"1998","journal-title":"Infect. 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