{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:53:17Z","timestamp":1791355997843,"version":"4.3.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Shigella, a Gram-negative invasive enteropathogenic bacterium responsible for bacillary dysentery, causes the rupture, invasion, and inflammatory destruction of the human colonic mucosa. We explored the mechanisms of protection mediated by Shigella LPS-specific secretory IgA (SIgA), the major mucosal Ab induced upon natural infection. Bacteria, SIgA, or SIgA-S. flexneri immune complexes were administered into rabbit ligated intestinal loops containing a Peyer\u2019s patch. After 8 h, localizations of bacteria, SIgA, and SIgA-S. flexneri immune complexes were examined by immunohistochemistry and confocal microscopy imaging. We found that anti-Shigella LPS SIgA, mainly via immune exclusion, prevented Shigella-induced inflammation responsible for the destruction of the intestinal barrier. Besides this luminal trapping, a small proportion of SIgA-S. flexneri immune complexes were shown to enter the rabbit Peyer\u2019s patch and were internalized by dendritic cells of the subepithelial dome region. Local inflammatory status was analyzed by quantitative RT-PCR using newly designed primers for rabbit pro- and anti-inflammatory mediator genes. In Peyer\u2019s patches exposed to immune complexes, limited up-regulation of the expression of proinflammatory genes, including TNF-\u03b1, IL-6, Cox-2, and IFN-\u03b3, was observed, consistent with preserved morphology. In contrast, in Peyer\u2019s patches exposed to Shigella alone, high expression of the same mediators was measured, indicating that neutralizing SIgA dampens the proinflammatory properties of Shigella. These results show that in the form of immune complexes, SIgA guarantees both immune exclusion and neutralization of translocated bacteria, thus preserving the intestinal barrier integrity by preventing bacterial-induced inflammation. These findings add to the multiple facets of the noninflammatory properties of SIgA.<\/jats:p>","DOI":"10.4049\/jimmunol.0901838","type":"journal-article","created":{"date-parts":[[2009,10,14]],"date-time":"2009-10-14T21:31:53Z","timestamp":1255555913000},"page":"5879-5885","source":"Crossref","is-referenced-by-count":166,"title":["Secretory IgA-Mediated Neutralization of\n                    <i>Shigella flexneri<\/i>\n                    Prevents Intestinal Tissue Destruction by Down-Regulating Inflammatory Circuits"],"prefix":"10.1093","volume":"183","author":[{"given":"S\u00e9verine","family":"Boullier","sequence":"first","affiliation":[{"name":"Institut National de la Recherche Agronomique, Unit\u00e9 Mixte de Recherche 1225 , Toulouse,","place":["France"]},{"name":"Universit\u00e9 de Toulouse, Ecole Nationale V\u00e9t\u00e9rinaire Toulouse, Unit\u00e9 Mixte de Recherche 1225 , Toulouse,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Tanguy","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur , Paris,","place":["France"]},{"name":"INSERM U 786 , Paris,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalil A","family":"Kadaoui","sequence":"additional","affiliation":[{"name":"Laboratoire R&D du Service d\u2019Immunologie et d\u2019Allergie, Centre Hospitalier Universitaire Vaudois , Lausanne,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Caubet","sequence":"additional","affiliation":[{"name":"Institut National de la Recherche Agronomique, Unit\u00e9 Mixte de Recherche 1225 , Toulouse,","place":["France"]},{"name":"Universit\u00e9 de Toulouse, Ecole Nationale V\u00e9t\u00e9rinaire Toulouse, Unit\u00e9 Mixte de Recherche 1225 , Toulouse,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Sansonetti","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur , Paris,","place":["France"]},{"name":"INSERM U 786 , Paris,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaise","family":"Corth\u00e9sy","sequence":"additional","affiliation":[{"name":"Laboratoire R&D du Service d\u2019Immunologie et d\u2019Allergie, Centre Hospitalier Universitaire Vaudois , Lausanne,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armelle","family":"Phalipon","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pathog\u00e9nie Microbienne Mol\u00e9culaire, Institut Pasteur , Paris,","place":["France"]},{"name":"INSERM U 786 , Paris,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,11,1]]},"reference":[{"key":"2026012811151360600_R1","first-page":"153","article-title":"Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response.","volume-title":"Adv. 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