{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:21:38Z","timestamp":1790904098800,"version":"4.1.0"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Knowing the inherent stimulatory properties of the lipid moiety of bacterial lipoproteins, we first hypothesized that Brucella abortus outer membrane protein (Omp)16 lipoprotein would be able to elicit a protective immune response without the need of external adjuvants. In this study, we demonstrate that Omp16 administered by the i.p. route confers significant protection against B. abortus infection and that the protective response evoked is independent of the protein lipidation. To date, Omp16 is the first Brucella protein that without the requirement of external adjuvants is able to induce similar protection levels to the control live vaccine S19. Moreover, the protein portion of Omp16 (unlipidated Omp16 [U-Omp16]) elicits a protective response when administered by the oral route. Either systemic or oral immunization with U-Omp16 elicits a Th1-specific response. These abilities of U-Omp16 indicate that it is endowed with self-adjuvanting properties. The adjuvanticity of U-Omp16 could be explained, at least in part, by its capacity to activate dendritic cells in vivo. U-Omp16 is also able to stimulate dendritic cells and macrophages in vitro. The latter property and its ability to induce a protective Th1 immune response against B. abortus infection have been found to be TLR4 dependent. The facts that U-Omp16 is an oral protective Ag and possesses a mucosal self-adjuvanting property led us to develop a plant-made vaccine expressing U-Omp16. Our results indicate that plant-expressed recombinant U-Omp16 is able to confer protective immunity, when given orally, indicating that a plant-based oral vaccine expressing U-Omp16 could be a valuable approach to controlling this disease.<\/jats:p>","DOI":"10.4049\/jimmunol.0902209","type":"journal-article","created":{"date-parts":[[2010,3,29]],"date-time":"2010-03-29T23:34:10Z","timestamp":1269905650000},"page":"5200-5212","source":"Crossref","is-referenced-by-count":64,"title":["The Protein Moiety of\n                    <i>Brucella abortus<\/i>\n                    Outer Membrane Protein 16 Is a New Bacterial Pathogen-Associated Molecular Pattern That Activates Dendritic Cells In Vivo, Induces a Th1 Immune Response, and Is a Promising Self-Adjuvanting Vaccine against Systemic and Oral Acquired Brucellosis"],"prefix":"10.1093","volume":"184","author":[{"given":"Karina A","family":"Pasquevich","sequence":"first","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Garc\u00eda Samartino","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena M","family":"Coria","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia M","family":"Estein","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Department of Animal Healthcare and Preventive Medicine, School of Veterinary Sciences, National University of the Center of the Buenos Aires Province , Tandil,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Zwerdling","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s E","family":"Iba\u00f1ez","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Barrionuevo","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Souza","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais , Belo Horizonte-Minas Gerais,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia Barbosa","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais , Belo Horizonte-Minas Gerais,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Borkowski","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Darmstadt, Institute of Botany , Darmstadt,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Costa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais , Belo Horizonte-Minas Gerais,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heribert","family":"Warzecha","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Darmstadt, Institute of Botany , Darmstadt,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo H","family":"Giambartolomei","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Cassataro","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, Clinical Hospital Jos\u00e9 de San Mart\u00edn , School of Medicine"},{"name":"Institute of Studies on Humoral Immunity (National Council of Research), School of Pharmacy and Biochemistry, University of Buenos Aires , Buenos Aires"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,5,1]]},"reference":[{"key":"2026012808081091600_r1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/S1369-5274(00)00165-X","article-title":"Brucellosis: a worldwide zoonosis.","volume":"4","author":"Boschiroli","year":"2001","journal-title":"Curr. Opin. Microbiol."},{"key":"2026012808081091600_r2","first-page":"237","article-title":"Pathogenesis and cellular immunity in experimental murine brucellosis.","volume":"56","author":"Cheers","year":"1984","journal-title":"Dev. Biol. Stand."},{"key":"2026012808081091600_r3","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, present and future.","volume":"90","author":"Schurig","year":"2002","journal-title":"Vet. Microbiol."},{"key":"2026012808081091600_r4","doi-asserted-by":"crossref","first-page":"4899","DOI":"10.1128\/iai.61.11.4899-4901.1993","article-title":"Endogenous gamma interferon mediates resistance to Brucella abortus infection.","volume":"61","author":"Zhan","year":"1993","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r5","first-page":"147","article-title":"Vaccination against Brucella.","volume":"13","author":"Nicoletti","year":"1990","journal-title":"Adv. Biotechnol. Processes"},{"key":"2026012808081091600_r6","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1016\/j.vaccine.2004.02.041","article-title":"Adverse events in humans associated with accidental exposure to the livestock brucellosis vaccine RB51.","volume":"22","author":"Ashford","year":"2004","journal-title":"Vaccine"},{"key":"2026012808081091600_r7","first-page":"287","article-title":"Human brucellosis: review of an under-diagnosed animal transmitted disease.","volume":"34","author":"Thakur","year":"2002","journal-title":"J. Commun. Dis."},{"key":"2026012808081091600_r8","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1128\/IAI.01151-08","article-title":"Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection.","volume":"77","author":"Pasquevich","year":"2009","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r9","doi-asserted-by":"crossref","first-page":"5958","DOI":"10.1016\/j.vaccine.2007.05.049","article-title":"A DNA vaccine coding for the chimera BLSOmp31 induced a better degree of protection against B. ovis and a similar degree of protection against B. melitensis than Rev.1 vaccination.","volume":"25","author":"Cassataro","year":"2007","journal-title":"Vaccine"},{"key":"2026012808081091600_r10","doi-asserted-by":"crossref","first-page":"6721","DOI":"10.1016\/j.vaccine.2007.07.002","article-title":"Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB\/c mice.","volume":"25","author":"Delpino","year":"2007","journal-title":"Vaccine"},{"key":"2026012808081091600_r11","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1128\/IAI.74.5.2734-2741.2006","article-title":"Protective immunity elicited by a divalent DNA vaccine encoding both the L7\/L12 and Omp16 genes of Brucella abortus in BALB\/c mice.","volume":"74","author":"Luo","year":"2006","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r12","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/S0952-7915(00)00120-5","article-title":"Immunological concepts of vaccine adjuvant activity.","volume":"12","author":"Schijns","year":"2000","journal-title":"Curr. Opin. Immunol."},{"key":"2026012808081091600_r13","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.1016\/j.vaccine.2003.12.030","article-title":"Vaccine adjuvant: it makes the difference.","volume":"22","author":"Lima","year":"2004","journal-title":"Vaccine"},{"key":"2026012808081091600_r14","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.it.2008.09.006","article-title":"New horizons in adjuvants for vaccine development.","volume":"30","author":"Reed","year":"2009","journal-title":"Trends Immunol."},{"key":"2026012808081091600_r15","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/0923-2494(92)80067-U","article-title":"Synthetic lipopeptides as novel adjuvants.","volume":"143","author":"Bessler","year":"1992","journal-title":"Res. Immunol."},{"key":"2026012808081091600_r16","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.1128\/JB.188.8.2761-2773.2006","article-title":"A database of bacterial lipoproteins (DOLOP) with functional assignments to predicted lipoproteins.","volume":"188","author":"Babu","year":"2006","journal-title":"J. Bacteriol."},{"key":"2026012808081091600_r17","first-page":"949","article-title":"Lipoprotein-dependent and -independent immune responses to spirochetal infection.","volume":"12","author":"Salazar","year":"2005","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"2026012808081091600_r18","doi-asserted-by":"crossref","first-page":"7401","DOI":"10.4049\/jimmunol.173.12.7401","article-title":"TLR2 recognizes a bacterial lipopeptide through direct binding.","volume":"173","author":"Vasselon","year":"2004","journal-title":"J. Immunol."},{"key":"2026012808081091600_r19","doi-asserted-by":"crossref","first-page":"A23","DOI":"10.1016\/S0264-410X(02)00383-3","article-title":"Outer membrane protein A (OmpA): a new pathogen-associated molecular pattern that interacts with antigen presenting cells-impact on vaccine strategies.","volume":"20","author":"Jeannin","year":"2002","journal-title":"Vaccine"},{"key":"2026012808081091600_r20","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1038\/82751","article-title":"OmpA targets dendritic cells, induces their maturation and delivers antigen into the MHC class I presentation pathway.","volume":"1","author":"Jeannin","year":"2000","journal-title":"Nat. Immunol."},{"key":"2026012808081091600_r21","doi-asserted-by":"crossref","first-page":"3545","DOI":"10.4049\/jimmunol.174.6.3545","article-title":"Neisserial porin-induced dendritic cell activation is MyD88 and TLR2 dependent.","volume":"174","author":"Singleton","year":"2005","journal-title":"J. Immunol."},{"key":"2026012808081091600_r22","doi-asserted-by":"crossref","first-page":"18243","DOI":"10.1073\/pnas.0608545103","article-title":"Sustained high-titer antibody responses induced by conjugating a malarial vaccine candidate to outer-membrane protein complex.","volume":"103","author":"Wu","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012808081091600_r23","first-page":"p. 41","article-title":"Relationship between animal and human disease","volume-title":"Brucellosis: clinical and laboratory aspects.","author":"Nicoetti","year":"1989"},{"key":"2026012808081091600_r24","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1128\/IAI.71.5.2326-2330.2003","article-title":"Brucella abortus RB51 induces protection in mice orally infected with the virulent strain B. abortus 2308.","volume":"71","author":"Pasquali","year":"2003","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r25","doi-asserted-by":"crossref","first-page":"4534","DOI":"10.1128\/iai.64.11.4534-4541.1996","article-title":"Immune responses and resistance to brucellosis in mice vaccinated orally with Brucella abortus RB51.","volume":"64","author":"Stevens","year":"1996","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r26","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1080\/10611860410001670035","article-title":"Induction of partial protection in mice after oral administration of Lactococcus lactis producing Brucella abortus L7\/L12 antigen.","volume":"11","author":"Pontes","year":"2003","journal-title":"J. Drug Target."},{"key":"2026012808081091600_r27","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1128\/IAI.70.4.1915-1923.2002","article-title":"Yersinia enterocolitica as a vehicle for a naked DNA vaccine encoding Brucella abortus bacterioferritin or P39 antigen.","volume":"70","author":"Al-Mariri","year":"2002","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r28","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1128\/IAI.00952-06","article-title":"A bile salt hydrolase of Brucella abortus contributes to the establishment of a successful infection through the oral route in mice.","volume":"75","author":"Delpino","year":"2007","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r29","doi-asserted-by":"crossref","first-page":"3633","DOI":"10.1128\/iai.62.9.3633-3639.1994","article-title":"Molecular cloning, nucleotide sequence, and occurrence of a 16.5-kilodalton outer membrane protein of Brucella abortus with similarity to pal lipoproteins.","volume":"62","author":"Tibor","year":"1994","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r30","doi-asserted-by":"crossref","first-page":"4960","DOI":"10.1128\/IAI.67.9.4960-4962.1999","article-title":"Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins.","volume":"67","author":"Tibor","year":"1999","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r31","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1128\/iai.64.1.100-107.1996","article-title":"Molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of Brucella abortus.","volume":"64","author":"Tibor","year":"1996","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r32","doi-asserted-by":"crossref","first-page":"4635","DOI":"10.4049\/jimmunol.173.7.4635","article-title":"Lipoproteins, not lipopolysaccharide, are the key mediators of the proinflammatory response elicited by heat-killed Brucella abortus.","volume":"173","author":"Giambartolomei","year":"2004","journal-title":"J. Immunol."},{"key":"2026012808081091600_r33","doi-asserted-by":"crossref","first-page":"5750","DOI":"10.1128\/IAI.71.10.5750-5755.2003","article-title":"Brucella lumazine synthase elicits a mixed Th1-Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used.","volume":"71","author":"Velikovsky","year":"2003","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r34","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1128\/iai.62.2.632-638.1994","article-title":"Protective immunity to Brucella ovis in BALB\/c mice following recovery from primary infection or immunization with subcellular vaccines.","volume":"62","author":"Jim\u00e9nez de Bag\u00fc\u00e9s","year":"1994","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r35","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0022-1759(98)00204-X","article-title":"An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow.","volume":"223","author":"Lutz","year":"1999","journal-title":"J. Immunol. Methods"},{"key":"2026012808081091600_r36","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.4049\/jimmunol.180.2.1080","article-title":"Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.","volume":"180","author":"Macedo","year":"2008","journal-title":"J. Immunol."},{"key":"2026012808081091600_r37","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1099\/00221287-148-10-3155","article-title":"NRAMP1- or cytokine-induced bacteriostasis of Mycobacterium avium by mouse macrophages is independent of the respiratory burst.","volume":"148","author":"Gomes","year":"2002","journal-title":"Microbiology"},{"key":"2026012808081091600_r38","doi-asserted-by":"crossref","first-page":"6852","DOI":"10.1073\/pnas.0400149101","article-title":"In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium.","volume":"101","author":"Marillonnet","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012808081091600_r39","first-page":"664","article-title":"Enhanced recovery of transformants of Agrobacterium tumefaciens after freeze-thaw transformation and drug selection.","volume":"16","author":"Chen","year":"1994","journal-title":"Biotechniques"},{"issue":"Web Server issue","key":"2026012808081091600_r40","first-page":"W465\u2013469","article-title":"Phylogeny.fr: robust phylogenetic analysis for the non-specialist.","volume":"36","author":"Dereeper","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"2026012808081091600_r41","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"2026012808081091600_r42","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S1473-3099(02)00318-3","article-title":"Lipopeptide vaccines\u2014yesterday, today, and tomorrow.","volume":"2","author":"BenMohamed","year":"2002","journal-title":"Lancet Infect. Dis."},{"key":"2026012808081091600_r43","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1615\/CritRevImmunol.v26.i5.30","article-title":"Host immune responses to the intracellular bacteria Brucella: does the bacteria instruct the host to facilitate chronic infection?","volume":"26","author":"Baldwin","year":"2006","journal-title":"Crit. Rev. Immunol."},{"key":"2026012808081091600_r44","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.imlet.2007.09.007","article-title":"Unique functions of splenic CD8alpha+ dendritic cells during infection with intracellular pathogens.","volume":"114","author":"Neuenhahn","year":"2007","journal-title":"Immunol. Lett."},{"key":"2026012808081091600_r45","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.immuni.2008.08.008","article-title":"In vivo induction of immune responses to pathogens by conventional dendritic cells.","volume":"29","author":"L\u00f3pez-Bravo","year":"2008","journal-title":"Immunity"},{"key":"2026012808081091600_r46","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/j.cell.2006.02.019","article-title":"Translating innate immunity into immunological memory: implications for vaccine development.","volume":"124","author":"Pulendran","year":"2006","journal-title":"Cell"},{"key":"2026012808081091600_r47","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/nri1391","article-title":"Toll-like receptor signalling.","volume":"4","author":"Akira","year":"2004","journal-title":"Nat. Rev. Immunol."},{"key":"2026012808081091600_r48","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1016\/j.molimm.2006.04.007","article-title":"Porin of Shigella dysenteriae activates mouse peritoneal macrophage through Toll-like receptors 2 and 6 to induce polarized type I response.","volume":"44","author":"Biswas","year":"2007","journal-title":"Mol. Immunol."},{"key":"2026012808081091600_r49","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/S0952-7915(97)80152-5","article-title":"Innate immunity: impact on the adaptive immune response.","volume":"9","author":"Medzhitov","year":"1997","journal-title":"Curr. Opin. Immunol."},{"key":"2026012808081091600_r50","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1128\/IAI.74.5.2676-2685.2006","article-title":"Outer membrane protein A of Escherichia coli O157:H7 stimulates dendritic cell activation.","volume":"74","author":"Torres","year":"2006","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r51","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1038\/nbt1261","article-title":"Vaccine manufacturing: challenges and solutions.","volume":"24","author":"Ulmer","year":"2006","journal-title":"Nat. Biotechnol."},{"key":"2026012808081091600_r52","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/S1567-5769(02)00030-9","article-title":"Lipopeptide adjuvants: monitoring and comparison of P3CSK4- and LPS-induced gene transcription.","volume":"2","author":"M\u00fcller","year":"2002","journal-title":"Int. Immunopharmacol."},{"key":"2026012808081091600_r53","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1002\/immu.200310006","article-title":"Outer membrane protein A renders dendritic cells and macrophages responsive to CCL21 and triggers dendritic cell migration to secondary lymphoid organs.","volume":"33","author":"Jeannin","year":"2003","journal-title":"Eur. J. Immunol."},{"key":"2026012808081091600_r54","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1016\/j.micinf.2008.07.029","article-title":"Borrelia burgdorferi lipoprotein BmpA activates pro-inflammatory responses in human synovial cells through a protein moiety.","volume":"10","author":"Yang","year":"2008","journal-title":"Microbes Infect."},{"key":"2026012808081091600_r55","doi-asserted-by":"crossref","first-page":"4816","DOI":"10.1128\/IAI.69.8.4816-4822.2001","article-title":"Protection of BALB\/c mice against Brucella abortus 544 challenge by vaccination with bacterioferritin or P39 recombinant proteins with CpG oligodeoxynucleotides as adjuvant.","volume":"69","author":"Al-Mariri","year":"2001","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r56","doi-asserted-by":"crossref","first-page":"3692","DOI":"10.1016\/j.vaccine.2007.01.066","article-title":"Liposomised recombinant ribosomal L7\/L12 protein protects BALB\/c mice against Brucella abortus 544 infection.","volume":"25","author":"Mallick","year":"2007","journal-title":"Vaccine"},{"key":"2026012808081091600_r57","doi-asserted-by":"crossref","first-page":"S266","DOI":"10.1086\/313883","article-title":"Improving vaccine performance with adjuvants.","volume":"30","author":"Vogel","year":"2000","journal-title":"Clin. Infect. Dis."},{"key":"2026012808081091600_r58","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/iai.63.1.264-270.1995","article-title":"Comparison of immune responses and resistance to brucellosis in mice vaccinated with Brucella abortus 19 or RB51.","volume":"63","author":"Stevens","year":"1995","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r59","doi-asserted-by":"crossref","first-page":"4031","DOI":"10.1128\/IAI.72.7.4031-4039.2004","article-title":"Oral vaccination with Brucella melitensis WR201 protects mice against intranasal challenge with virulent Brucella melitensis 16M.","volume":"72","author":"Izadjoo","year":"2004","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r60","doi-asserted-by":"crossref","first-page":"2048","DOI":"10.1128\/iai.58.7.2048-2055.1990","article-title":"Oral immunization of mice with attenuated Salmonella typhimurium containing a recombinant plasmid which codes for production of a 31-kilodalton protein of Brucella abortus.","volume":"58","author":"Stabel","year":"1990","journal-title":"Infect. Immun."},{"key":"2026012808081091600_r61","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S1286-4579(00)01350-2","article-title":"Immunity and protection against Brucella abortus.","volume":"3","author":"Golding","year":"2001","journal-title":"Microbes Infect."},{"key":"2026012808081091600_r62","first-page":"17","article-title":"Dendritic cells: translating innate to adaptive immunity.","volume":"311","author":"Steinman","year":"2006","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"2026012808081091600_r63","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.0105-2896.2004.00144.x","article-title":"Modulating vaccine responses with dendritic cells and Toll-like receptors.","volume":"199","author":"Pulendran","year":"2004","journal-title":"Immunol. Rev."},{"key":"2026012808081091600_r64","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1084\/jem.189.3.587","article-title":"CD8alpha+ and CD8alpha- subclasses of dendritic cells direct the development of distinct T helper cells in vivo.","volume":"189","author":"Maldonado-L\u00f3pez","year":"1999","journal-title":"J. Exp. Med."},{"key":"2026012808081091600_r65","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1073\/pnas.96.3.1036","article-title":"Distinct dendritic cell subsets differentially regulate the class of immune response in vivo.","volume":"96","author":"Pulendran","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012808081091600_r66","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1038\/ni1112","article-title":"Toll-like receptor control of the adaptive immune responses.","volume":"5","author":"Iwasaki","year":"2004","journal-title":"Nat. Immunol."},{"key":"2026012808081091600_r67","doi-asserted-by":"crossref","first-page":"6777","DOI":"10.1016\/j.vaccine.2008.09.045","article-title":"Engagement of TLR signaling as adjuvant: towards smarter vaccine and beyond.","volume":"26","author":"Lahiri","year":"2008","journal-title":"Vaccine"},{"key":"2026012808081091600_r68","doi-asserted-by":"crossref","first-page":"10229","DOI":"10.1074\/jbc.M100099200","article-title":"The extra domain A of fibronectin activates Toll-like receptor 4.","volume":"276","author":"Okamura","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2026012808081091600_r69","doi-asserted-by":"crossref","first-page":"748","DOI":"10.4049\/jimmunol.178.2.748","article-title":"The extra domain A from fibronectin targets antigens to TLR4-expressing cells and induces cytotoxic T cell responses in vivo.","volume":"178","author":"Lasarte","year":"2007","journal-title":"J. Immunol."},{"key":"2026012808081091600_r70","doi-asserted-by":"crossref","first-page":"20847","DOI":"10.1074\/jbc.M200425200","article-title":"The endoplasmic reticulum-resident heat shock protein Gp96 activates dendritic cells via the Toll-like receptor 2\/4 pathway.","volume":"277","author":"Vabulas","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2026012808081091600_r71","doi-asserted-by":"crossref","first-page":"5672","DOI":"10.4049\/jimmunol.174.9.5672","article-title":"The secreted peptidyl prolyl cis,trans-isomerase HP0175 of Helicobacter pylori induces apoptosis of gastric epithelial cells in a TLR4- and apoptosis signal-regulating kinase 1-dependent manner.","volume":"174","author":"Basak","year":"2005","journal-title":"J. Immunol."},{"key":"2026012808081091600_r72","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1189\/jlb.0308215","article-title":"TLR4-mediated activation of dendritic cells by the heat shock protein DnaK from Francisella tularensis.","volume":"84","author":"Ashtekar","year":"2008","journal-title":"J. Leukoc. Biol."},{"key":"2026012808081091600_r73","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1111\/j.1365-2567.2005.02155.x","article-title":"Comparative cell signalling activity of ultrapure recombinant chaperonin 60 proteins from prokaryotes and eukaryotes.","volume":"115","author":"Maguire","year":"2005","journal-title":"Immunology"},{"key":"2026012808081091600_r74","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.4049\/jimmunol.176.4.2366","article-title":"A polymeric bacterial protein activates dendritic cells via TLR4.","volume":"176","author":"Berguer","year":"2006","journal-title":"J. Immunol."},{"key":"2026012808081091600_r75","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1038\/ni1528","article-title":"Pathogen subversion of cell-intrinsic innate immunity.","volume":"8","author":"Roy","year":"2007","journal-title":"Nat. Immunol."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/184\/9\/5200\/62712030\/ji_0902209.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/184\/9\/5200\/62712030\/ji_0902209.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T13:08:45Z","timestamp":1769605725000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/184\/9\/5200\/8013315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,1]]},"references-count":75,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2010,5,1]]},"published-print":{"date-parts":[[2010,5,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.0902209","relation":{},"ISSN":["1550-6606","0022-1767"],"issn-type":[{"value":"1550-6606","type":"electronic"},{"value":"0022-1767","type":"print"}],"subject":[],"published-other":{"date-parts":[[2010,5]]},"published":{"date-parts":[[2010,5,1]]}}}