{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T19:10:23Z","timestamp":1743189023424,"version":"3.40.3"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chronic lymphocytic leukemia (CLL) cells that use IgH encoded by IGHV3-21 and that have a particular stereotypic third CDR (HCDR3), DANGMDV (motif-1), almost invariably express Ig L chains (IgL) encoded by IGLV3-21, whereas CLL that use IGHV3-21\u2013encoded IgH with another stereotypic HCDR3, DPSFYSSSWTLFDY (motif-2), invariably express \u03ba-IgL encoded by IGKV3-20. This nonstochastic pairing could reflect steric factors that preclude these IgH from pairing with other IgL or selection for an Ig with a particular Ag-binding activity. We generated rIg with IGHV3-21\u2013encoded IgH with HCDR3 motif-1 or -2 and IgL encoded by IGKV3-20 or IGLV3-21. Each IgH paired equally well with matched or mismatched \u03ba- or \u03bb-IgL to form functional Ig, which we screened for binding to an array of different Ags. Ig with IGLV3-21\u2013encoded \u03bb-IgL could bind with an affinity of \u223c2 \u00d7 10\u22126 M to protein L, a cell-wall protein of Peptostreptococcus magnus, independent of the IgH, indicating that protein L is a superantigen for IGLV3-21\u2013encoded \u03bb-IgL. We also detected Ig binding to cofilin, a highly conserved actin-binding protein. However, cofilin binding was independent of native pairing of IgH and IgL and was not specific for Ig with IgH encoded by IGHV3-21. We conclude that steric factors or the binding activity for protein L or cofilin cannot account for the nonstochastic pairing of IgH and IgL observed for the stereotypic Ig made by CLL cells that express IGHV3-21.<\/jats:p>","DOI":"10.4049\/jimmunol.0902875","type":"journal-article","created":{"date-parts":[[2011,4,28]],"date-time":"2011-04-28T02:57:54Z","timestamp":1303959474000},"page":"6338-6344","source":"Crossref","is-referenced-by-count":4,"title":["Analyses of Recombinant Stereotypic <i>IGHV3-21<\/i>\u2013Encoded Antibodies Expressed in Chronic Lymphocytic Leukemia"],"prefix":"10.1093","volume":"186","author":[{"given":"Emanuela M","family":"Ghia","sequence":"first","affiliation":[{"name":"Moores University of California San Diego Cancer Center , La Jolla, CA 92093"},{"name":"Chronic Lymphocytic Leukemia Research Consortium , La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"II","given":"George F","family":"Widhopf","sequence":"additional","affiliation":[{"name":"Moores University of California San Diego Cancer Center , La Jolla, CA 92093"},{"name":"Chronic Lymphocytic Leukemia Research Consortium , La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Z","family":"Rassenti","sequence":"additional","affiliation":[{"name":"Moores University of California San Diego Cancer Center , La Jolla, CA 92093"},{"name":"Chronic Lymphocytic Leukemia Research Consortium , La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas J","family":"Kipps","sequence":"additional","affiliation":[{"name":"Moores University of California San Diego Cancer Center , La Jolla, CA 92093"},{"name":"Chronic Lymphocytic Leukemia Research Consortium , La Jolla, CA 92093"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,6,1]]},"reference":[{"key":"2025032411281366900_r1","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1172\/JCI19399","article-title":"Remarkably similar antigen receptors among a subset of patients with chronic lymphocytic leukemia","volume":"113","author":"Ghiotto","year":"2004","journal-title":"J. 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