{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T10:19:40Z","timestamp":1772619580805,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In Guillain-Barr\u00e9 syndrome (GBS), ganglioside mimicry of Campylobacter jejuni lipo-oligosaccharide (LOS) drives the production of cross-reactive Abs to peripheral nerve gangliosides. We determined whether sialic acid residues in C. jejuni LOS modulate dendritic cell (DC) activation and subsequent B cell proliferation as a possible mechanism for the aberrant humoral immune response in GBS. Highly purified sialylated LOS of C. jejuni isolates from three GBS patients induced human DC maturation and secretion of inflammatory cytokines that were inhibited by anti-TLR4 neutralizing Abs. The extent of TLR4 signaling and DC activation was greater with LOS of the wild type isolates than with nonsialylated LOS of the corresponding sialyltransferase gene knockout (cst-II mutant) strains, indicating that sialylation boosts the DC response to C. jejuni LOS. Supernatants of LOS-activated DCs induced B cell proliferation after cross-linking of surface Igs in the absence of T cells. Lower B cell proliferation indices were found with DC supernatants after DC stimulation with cst-II mutant or neuraminidase desialylated LOS. This study showed that sialylation of C. jejuni LOS enhances human DC activation and subsequent B cell proliferation, which may contribute to the development of cross-reactive anti-ganglioside Abs found in GBS patients following C. jejuni infection.<\/jats:p>","DOI":"10.4049\/jimmunol.0903014","type":"journal-article","created":{"date-parts":[[2010,6,5]],"date-time":"2010-06-05T02:41:27Z","timestamp":1275705687000},"page":"748-755","source":"Crossref","is-referenced-by-count":69,"title":["TLR4-Mediated Sensing of <i>Campylobacter jejuni<\/i> by Dendritic Cells Is Determined by Sialylation"],"prefix":"10.1093","volume":"185","author":[{"given":"Mark L","family":"Kuijf","sequence":"first","affiliation":[{"name":"Department of Neurology, University Medical Center , Amsterdam ,","place":["The Netherlands"]},{"name":"Department of Immunology, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janneke N","family":"Samsom","sequence":"additional","affiliation":[{"name":"Division Gastroenterology and Nutrition, Department of Pediatrics, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wouter","family":"van Rijs","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medical Center , Amsterdam ,","place":["The Netherlands"]},{"name":"Department of Immunology, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marieke","family":"Bax","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology and Immunology, Vrije University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Huizinga","sequence":"additional","affiliation":[{"name":"Department of Immunology, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astrid P","family":"Heikema","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, University Medical Center , Rotterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter A","family":"van Doorn","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"van Belkum","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, University Medical Center , Rotterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvette","family":"van Kooyk","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology and Immunology, Vrije University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter C","family":"Burgers","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theo M","family":"Luider","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert P","family":"Endtz","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, University Medical Center , Rotterdam ,","place":["The Netherlands"]},{"name":"International Centre for Diarrhoeal Disease Research , Dhaka ,","place":["Bangladesh"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward E S","family":"Nieuwenhuis","sequence":"additional","affiliation":[{"name":"Division Gastroenterology and Nutrition, Department of Pediatrics, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart C","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Department of Neurology, University Medical Center , Amsterdam ,","place":["The Netherlands"]},{"name":"Department of Immunology, University Medical Center , Amsterdam ,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,7,1]]},"reference":[{"key":"2025032411364561800_r1","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1212\/WNL.51.4.1110","article-title":"The spectrum of antecedent infections in Guillain-Barr\u00e9 syndrome: a 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