{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T14:00:56Z","timestamp":1758981656429,"version":"3.40.3"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Increased proportions of naive B cell subset and B cells defined as CD27negCD21negCD38neg are frequently found in patients with common variable immunodeficiency (CVID) syndrome. Current methods of polychromatic flow cytometry and PCR-based detection of \u03ba deletion excision circles allow for fine definitions and replication history mapping of infrequent B cell subsets. We have analyzed B cells from 48 patients with CVID and 49 healthy controls to examine phenotype, frequency, and proliferation history of naive B cell subsets. Consistent with previous studies, we have described two groups of patients with normal (CVID-21norm) or increased (CVID-21lo) proportions of CD27negCD21negCD38neg B cells. Upon further analyses, we found two discrete subpopulations of this subset based on the expression of CD24. The B cell subsets showed a markedly increased proliferation in CVID-21lo patients as compared with healthy controls, suggesting developmental arrest rather than increased bone marrow output. Furthermore, when we analyzed CD21pos naive B cells, we found two different subpopulations based on IgM and CD24 expression. They correspond to follicular (FO) I and FO II cells previously described in mice. FO I subset is significantly underrepresented in CVID-21lo patients. A comparison of the replication history of naive B cell subsets in CVID patients and healthy controls implies refined naive B cell developmental scheme, in which human transitional B cells develop into FO II and FO I. We propose that the CD27negCD21negCD38neg B cells increased in some of the CVID patients originate from the two FO subsets after loss of CD21 expression.<\/jats:p>","DOI":"10.4049\/jimmunol.0903876","type":"journal-article","created":{"date-parts":[[2010,11,2]],"date-time":"2010-11-02T05:29:04Z","timestamp":1288675744000},"page":"6431-6438","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of Lymphocyte Subsets in Patients with Common Variable Immunodeficiency Reveals Subsets of Naive Human B Cells Marked by CD24 Expression"],"prefix":"10.1093","volume":"185","author":[{"given":"Marcela","family":"Vlkov\u00e1","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology and Allergology, St. Anne\u2019s University Hospital and Faculty of Medicine, Masaryk University , Brno ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Fro\u0148kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology 2nd Medical Faculty, Charles University and University Hospital Motol , Prague ,","place":["Czech Republic"]},{"name":"Childhood Leukemia Investigation Prague , Prague ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Kanderov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology 2nd Medical Faculty, Charles University and University Hospital Motol , Prague ,","place":["Czech Republic"]},{"name":"Childhood Leukemia Investigation Prague , Prague ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ale\u0161","family":"Janda","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology 2nd Medical Faculty, Charles University and University Hospital Motol , Prague ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0160\u00e1rka","family":"R\u016f\u017ei\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Diagnostics of Autoimmune Diseases, Institute of Biotechnology of Academy of Sciences of the Czech Republic, v.v.i. , Prague ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u0159\u00ed","family":"Litzman","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Allergology, St. Anne\u2019s University Hospital and Faculty of Medicine, Masaryk University , Brno ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"\u0160ediv\u00e1","sequence":"additional","affiliation":[{"name":"Department of Immunology, 2nd Medical School, Charles University and University Hospital Motol , Prague ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Kalina","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology 2nd Medical Faculty, Charles University and University Hospital Motol , Prague ,","place":["Czech Republic"]},{"name":"Childhood Leukemia Investigation Prague , Prague ,","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,12,1]]},"reference":[{"key":"2025032412112726900_r1","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1006\/clim.1999.4725","article-title":"Common variable immunodeficiency: clinical and immunological features of 248 patients","volume":"92","author":"Cunningham-Rundles","year":"1999","journal-title":"Clin. 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