{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:19:05Z","timestamp":1790263145904,"version":"4.1.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Lymphocyte recruitment to intestinal tissues depends on \u03b27 integrins. In this study, we studied disease severity and lymphocyte recruitment into the small intestine in SAMP1\/YitFc mice, which develop chronic ileitis with similarity to human Crohn\u2019s disease. To assess the role of \u03b27 integrins in chronic ileitis, we generated SAMP1\/YitFc lacking \u03b27 integrins (SAMP1\/YitFc Itgb7\u2212\/\u2212) using a congenic strain developed via marker-assisted selection. We analyzed ileal inflammation in SAMP1\/YitFc and SAMP1\/YitFc Itgb7\u2212\/\u2212 mice by histopathology and the distribution of T and B lymphocytes in the mesenteric lymph nodes (MLNs) by flow cytometry. Short-term (18 h) adoptive transfer experiments were used to study the in vivo homing capacity of T and B lymphocytes. In both young (&amp;lt;20 wk) and old (20\u201350 wk) SAMP1\/YitFc Itgb7\u2212\/\u2212 mice, ileitis was reduced by 30\u201350% compared with SAMP1\/YitFc mice. SAMP1\/YitFc Itgb7\u2212\/\u2212 mice showed a dramatic 67% reduction in the size of their MLNs, which was caused by a 85% reduction in lymphocyte numbers and reduced short-term B cell homing. Flow cytometric analysis revealed a highly significant decrease in the percentage of B cells in MLNs of SAMP1\/YitFc Itgb7\u2212\/\u2212 mice. Cotransfer of SAMP1\/YitFc MLN B cells but not SAMP1\/YitFc Itgb7\u2212\/\u2212 MLN B cells along with CD4+ T cells resulted in exacerbated ileitis severity in SCID mice. Our findings suggest that \u03b27 integrins play an essential role in spontaneous chronic ileitis in vivo by promoting homing of disease-exacerbating B cells to MLNs and other intestinal tissues.<\/jats:p>","DOI":"10.4049\/jimmunol.0903938","type":"journal-article","created":{"date-parts":[[2010,10,7]],"date-time":"2010-10-07T03:17:49Z","timestamp":1286421469000},"page":"5561-5568","source":"Crossref","is-referenced-by-count":25,"title":["\u03b27 Integrin Deficiency Suppresses B Cell Homing and Attenuates Chronic Ileitis in SAMP1\/YitFc Mice"],"prefix":"10.1093","volume":"185","author":[{"given":"Gezahegn","family":"Gorfu","sequence":"first","affiliation":[{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus","family":"Rivera-Nieves","sequence":"additional","affiliation":[{"name":"Mucosal Inflammation Program, Division of Gastroenterology, Department of Internal Medicine, University of Colorado Health Sciences Center , Denver, CO 80206"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Hoang","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Liver Disease, Case Western Reserve University , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek W","family":"Abbott","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keely","family":"Arbenz-Smith","sequence":"additional","affiliation":[{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W","family":"Azar","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California , San Diego, CA, 92103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa T","family":"Pizarro","sequence":"additional","affiliation":[{"name":"Department of Pathology, Case Western Reserve University , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Cominelli","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Liver Disease, Case Western Reserve University , Cleveland, OH 44106"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcia","family":"McDuffie","sequence":"additional","affiliation":[{"name":"Microbiology and Medicine, University of Virginia Health Sciences Center , Charlottesville, VA 22908"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Ley","sequence":"additional","affiliation":[{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,11,1]]},"reference":[{"key":"2025032412040717300_r1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1172\/JCI30587","article-title":"The fundamental basis of inflammatory bowel disease","volume":"117","author":"Strober","year":"2007","journal-title":"J. Clin. Invest."},{"key":"2025032412040717300_r2","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/nature06005","article-title":"Unravelling the pathogenesis of inflammatory bowel disease","volume":"448","author":"Xavier","year":"2007","journal-title":"Nature"},{"key":"2025032412040717300_r3","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1002\/ibd.20453","article-title":"Lymphocyte homing and its role in the pathogenesis of IBD","volume":"14","author":"Eksteen","year":"2008","journal-title":"Inflamm. Bowel Dis."},{"key":"2025032412040717300_r4","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1002\/ibd.20501","article-title":"Leukocyte adhesion molecules in animal models of inflammatory bowel disease","volume":"14","author":"Rivera-Nieves","year":"2008","journal-title":"Inflamm. Bowel Dis."},{"key":"2025032412040717300_r5","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.it.2008.08.003","article-title":"T-cell recruitment to the intestinal mucosa","volume":"29","author":"Agace","year":"2008","journal-title":"Trends Immunol."},{"key":"2025032412040717300_r6","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0065-2776(08)60022-X","article-title":"Lymphocyte trafficking and regional immunity","volume":"72","author":"Butcher","year":"1999","journal-title":"Adv. Immunol."},{"key":"2025032412040717300_r7","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1056\/NEJM200010053431407","article-title":"T-cell function and migration. Two sides of the same coin","volume":"343","author":"von Andrian","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"2025032412040717300_r8","doi-asserted-by":"crossref","first-page":"836","DOI":"10.2174\/156652409789105525","article-title":"Role of beta7 integrins in intestinal lymphocyte homing and retention","volume":"9","author":"Gorfu","year":"2009","journal-title":"Curr. Mol. Med."},{"key":"2025032412040717300_r9","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/0092-8674(91)90279-8","article-title":"Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity","volume":"67","author":"Butcher","year":"1991","journal-title":"Cell"},{"key":"2025032412040717300_r10","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0092-8674(94)90337-9","article-title":"Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm","volume":"76","author":"Springer","year":"1994","journal-title":"Cell"},{"key":"2025032412040717300_r11","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1038\/nri2156","article-title":"Getting to the site of inflammation: the leukocyte adhesion cascade updated","volume":"7","author":"Ley","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"2025032412040717300_r12","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0092-8674(93)90305-A","article-title":"Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1","volume":"74","author":"Berlin","year":"1993","journal-title":"Cell"},{"key":"2025032412040717300_r13","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1038\/372190a0","article-title":"Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin","volume":"372","author":"Cepek","year":"1994","journal-title":"Nature"},{"key":"2025032412040717300_r14","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/382366a0","article-title":"Critical role for beta7 integrins in formation of the gut-associated lymphoid tissue","volume":"382","author":"Wagner","year":"1996","journal-title":"Nature"},{"key":"2025032412040717300_r15","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1084\/jem.192.5.761","article-title":"Lymphocyte CC chemokine receptor 9 and epithelial thymus-expressed chemokine (TECK) expression distinguish the small intestinal immune compartment: Epithelial expression of tissue-specific chemokines as an organizing principle in regional immunity","volume":"192","author":"Kunkel","year":"2000","journal-title":"J. Exp. Med."},{"key":"2025032412040717300_r16","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/jlb.65.3.349","article-title":"Expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in acute and chronic inflammation","volume":"65","author":"Connor","year":"1999","journal-title":"J. Leukoc. Biol."},{"key":"2025032412040717300_r17","doi-asserted-by":"crossref","first-page":"3282","DOI":"10.4049\/jimmunol.152.7.3282","article-title":"Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo","volume":"152","author":"Hamann","year":"1994","journal-title":"J. Immunol."},{"key":"2025032412040717300_r18","doi-asserted-by":"crossref","first-page":"924","DOI":"10.1053\/j.gastro.2009.05.039","article-title":"Mucosal addressin cell-adhesion molecule-1 controls plasma-cell migration and function in the small intestine of mice","volume":"137","author":"Schippers","year":"2009","journal-title":"Gastroenterology"},{"key":"2025032412040717300_r19","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.4049\/jimmunol.166.3.1894","article-title":"Protective intestinal anti-rotavirus B cell immunity is dependent on alpha 4 beta 7 integrin expression but does not require IgA antibody production","volume":"166","author":"Kuklin","year":"2001","journal-title":"J. Immunol."},{"key":"2025032412040717300_r20","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1046\/j.1365-2249.2002.01892.x","article-title":"beta7 Integrin expression is not required for the localization of T cells to the intestine and colitis pathogenesis","volume":"129","author":"Sydora","year":"2002","journal-title":"Clin. Exp. Immunol."},{"key":"2025032412040717300_r21","doi-asserted-by":"crossref","first-page":"2526","DOI":"10.1172\/JCI31570","article-title":"Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut","volume":"117","author":"Park","year":"2007","journal-title":"J. Clin. Invest."},{"key":"2025032412040717300_r22","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.4049\/jimmunol.158.5.2099","article-title":"Monoclonal antibodies specific for beta 7 integrin and mucosal addressin cell adhesion molecule-1 (MAdCAM-1) reduce inflammation in the colon of scid mice reconstituted with CD45RBhigh CD4+ T cells","volume":"158","author":"Picarella","year":"1997","journal-title":"J. Immunol."},{"key":"2025032412040717300_r23","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1053\/j.gastro.2008.02.085","article-title":"Role of beta7 integrin and the chemokine\/chemokine receptor pair CCL25\/CCR9 in modeled TNF-dependent Crohn\u2019s disease","volume":"134","author":"Apostolaki","year":"2008","journal-title":"Gastroenterology"},{"key":"2025032412040717300_r24","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.4049\/jimmunol.174.4.2343","article-title":"L-selectin, alpha 4 beta 1, and alpha 4 beta 7 integrins participate in CD4+ T cell recruitment to chronically inflamed small intestine","volume":"174","author":"Rivera-Nieves","year":"2005","journal-title":"J. Immunol."},{"key":"2025032412040717300_r25","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1172\/JCI200420855","article-title":"Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease","volume":"114","author":"Olson","year":"2004","journal-title":"J. Clin. Invest."},{"key":"2025032412040717300_r26","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1053\/gast.2003.50148","article-title":"Emergence of perianal fistulizing disease in the SAMP1\/YitFc mouse, a spontaneous model of chronic ileitis","volume":"124","author":"Rivera-Nieves","year":"2003","journal-title":"Gastroenterology"},{"key":"2025032412040717300_r27","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1136\/gut.43.1.71","article-title":"Inflammatory bowel disease-like enteritis and caecitis in a senescence accelerated mouse P1\/Yit strain","volume":"43","author":"Matsumoto","year":"1998","journal-title":"Gut"},{"key":"2025032412040717300_r28","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0016-5085(03)00876-X","article-title":"Identification of a quantitative trait locus for ileitis in a spontaneous mouse model of Crohn\u2019s disease: SAMP1\/YitFc","volume":"125","author":"Kozaiwa","year":"2003","journal-title":"Gastroenterology"},{"key":"2025032412040717300_r29","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1053\/gast.2001.29568","article-title":"Antibody blockade of ICAM-1 and VCAM-1 ameliorates inflammation in the SAMP-1\/Yit adoptive transfer model of Crohn\u2019s disease in mice","volume":"121","author":"Burns","year":"2001","journal-title":"Gastroenterology"},{"key":"2025032412040717300_r30","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1172\/JCI10956","article-title":"Th1-type responses mediate spontaneous ileitis in a novel murine model of Crohn\u2019s disease","volume":"107","author":"Kosiewicz","year":"2001","journal-title":"J. Clin. Invest."},{"key":"2025032412040717300_r31","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1053\/j.gastro.2004.11.053","article-title":"Proinflammatory effects of TH2 cytokines in a murine model of chronic small intestinal inflammation","volume":"128","author":"Bamias","year":"2005","journal-title":"Gastroenterology"},{"key":"2025032412040717300_r32","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1053\/j.gastro.2006.08.031","article-title":"Antibody blockade of CCL25\/CCR9 ameliorates early but not late chronic murine ileitis","volume":"131","author":"Rivera-Nieves","year":"2006","journal-title":"Gastroenterology"},{"key":"2025032412040717300_r33","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1365-2249.2005.02742.x","article-title":"In vivo demonstration of T lymphocyte migration and amelioration of ileitis in intestinal mucosa of SAMP1\/Yit mice by the inhibition of MAdCAM-1","volume":"140","author":"Matsuzaki","year":"2005","journal-title":"Clin. Exp. Immunol."},{"key":"2025032412040717300_r34","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.4049\/jimmunol.161.5.2449","article-title":"The roles of L-selectin, beta 7 integrins, and P-selectin in leukocyte rolling and adhesion in high endothelial venules of Peyer\u2019s patches","volume":"161","author":"Kunkel","year":"1998","journal-title":"J. Immunol."},{"key":"2025032412040717300_r35","doi-asserted-by":"crossref","first-page":"3832","DOI":"10.1002\/(SICI)1521-4141(199811)28:11<3832::AID-IMMU3832>3.0.CO;2-J","article-title":"L-selectin and beta7 integrin synergistically mediate lymphocyte migration to mesenteric lymph nodes","volume":"28","author":"Wagner","year":"1998","journal-title":"Eur. J. Immunol."},{"key":"2025032412040717300_r36","doi-asserted-by":"crossref","first-page":"6638","DOI":"10.4049\/jimmunol.161.12.6638","article-title":"Efficient lymphocyte migration across high endothelial venules of mouse Peyer\u2019s patches requires overlapping expression of L-selectin and beta7 integrin","volume":"161","author":"Steeber","year":"1998","journal-title":"J. Immunol."},{"key":"2025032412040717300_r37","doi-asserted-by":"crossref","first-page":"5113","DOI":"10.4049\/jimmunol.160.10.5113","article-title":"Intrinsic differences in L-selectin expression levels affect T and B lymphocyte subset-specific recirculation pathways","volume":"160","author":"Tang","year":"1998","journal-title":"J. Immunol."},{"key":"2025032412040717300_r38","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1016\/j.cgh.2008.06.007","article-title":"Treatment of active Crohn\u2019s disease with MLN0002, a humanized antibody to the alpha4beta7 integrin","volume":"6","author":"Feagan","year":"2008","journal-title":"Clin. Gastroenterol. Hepatol."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/185\/9\/5561\/61638017\/0903938.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/185\/9\/5561\/61638017\/0903938.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T01:53:58Z","timestamp":1743213238000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/185\/9\/5561\/7981897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,1]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,11,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.0903938","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2010,11]]},"published":{"date-parts":[[2010,11,1]]}}}