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In this article, we report the generation and characterization of T cell-specific TRAF3\u2212\/\u2212 mice, in which the traf3 gene was deleted from thymocytes and T cells. Ablation of TRAF3 in the T cell lineage did not affect CD4 or CD8 T cell populations in secondary lymphoid organs or the numbers or proportions of CD4+,CD8+ or double-positive or double-negative thymocytes, except that the T cell-specific TRAF3\u2212\/\u2212 mice had a 2-fold increase in FoxP3+ T cells. In striking contrast to mice lacking TRAF3 in B cells, the T cell TRAF3-deficient mice exhibited defective IgG1 responses to a T-dependent Ag, as well as impaired T cell-mediated immunity to infection with Listeria monocytogenes. Surprisingly, we found that TRAF3 was recruited to the TCR\/CD28 signaling complex upon costimulation and that TCR\/CD28-mediated proximal and distal signaling events were compromised by TRAF3 deficiency. These findings provide insights into the roles played by TRAF3 in T cell activation and T cell-mediated immunity.<\/jats:p>","DOI":"10.4049\/jimmunol.1000290","type":"journal-article","created":{"date-parts":[[2010,11,18]],"date-time":"2010-11-18T04:08:45Z","timestamp":1290053325000},"page":"143-155","source":"Crossref","is-referenced-by-count":84,"title":["TNF Receptor-Associated Factor 3 Is Required for T Cell-Mediated Immunity and TCR\/CD28 Signaling"],"prefix":"10.1093","volume":"186","author":[{"given":"Ping","family":"Xie","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Neuroscience, Rutgers University , Piscataway, NJ 08854"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary J","family":"Kraus","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, University of Iowa , Iowa City, IA 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura L","family":"Stunz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Neuroscience, Rutgers University , Piscataway, NJ 08854"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gail A","family":"Bishop","sequence":"additional","affiliation":[{"name":"Graduate Program in Immunology, University of Iowa , Iowa City, IA 52242"},{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"},{"name":"Department of Internal Medicine, University of Iowa , Iowa City, IA 52242"},{"name":"Veterans Affairs Medical Center , Iowa City, IA 52242"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,1,1]]},"reference":[{"key":"2025032410553173700_r1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s12026-007-0068-1","article-title":"Multiple roles of TRAF3 signaling in lymphocyte function","volume":"39","author":"Bishop","year":"2007","journal-title":"Immunol. 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