{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:11:51Z","timestamp":1791162711728,"version":"4.1.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>To assess the extent and nature of somatic categorical selection of CDR-3 of the Ig H chain (CDR-H3) content in peritoneal cavity (PerC) B cells, we analyzed the composition of VH7183DJC\u03bc transcripts derived from sorted PerC B-1a, B-1b, and B-2 cells. We divided these sequences into those that contained N nucleotides (N+) and those that did not (N\u2212) and then compared them with sequences cloned from sorted IgM+IgD+ B cells from neonatal liver and both wild-type and TdT-deficient adult bone marrow. We found that the PerC B-1a N\u2212 repertoire is enriched for the signatures of CDR-H3 sequences present in neonatal liver and shares many features with the B-1b N\u2212 repertoire, whereas the PerC B-1a N+, B-1b N+, and B-2 N+ repertoires are enriched for adult bone marrow sequence signatures. However, we also found several sequence signatures that were not shared with other mature perinatal or adult B cell subsets but were either unique or variably shared between the two or even among all three of the PerC subsets that we examined. These signatures included more sequences lacking N nucleotides in the B-2 population and an increased use of DH reading frame 2, which created CDR-H3s of greater average hydrophobicity. These findings provide support for both ontogenetic origin and shared Ag receptor-influenced selection as the mechanisms that shape the unique composition of the B-1a, B-1b, and B-2 repertoires. The PerC may thus serve as a general reservoir for B cells with Ag binding specificities that are uncommon in other mature compartments.<\/jats:p>","DOI":"10.4049\/jimmunol.1001423","type":"journal-article","created":{"date-parts":[[2010,10,19]],"date-time":"2010-10-19T04:19:16Z","timestamp":1287461956000},"page":"6085-6095","source":"Crossref","is-referenced-by-count":20,"title":["The Peritoneal Cavity B-2 Antibody Repertoire Appears To Reflect Many of the Same Selective Pressures That Shape the B-1a and B-1b Repertoires"],"prefix":"10.1093","volume":"185","author":[{"given":"Andre M","family":"Vale","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M","family":"Tanner","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L","family":"Schelonka","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxin","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Zemlin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Philipps University Marburg , Marburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G Larry","family":"Gartland","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Harry W","family":"Schroeder","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Alabama at Birmingham , Birmingham, AL 35294"},{"name":"Department of Microbiology, University of Alabama at Birmingham , Birmingham, AL 35294"},{"name":"Department of Genetics, University of Alabama at Birmingham , Birmingham, AL 35294"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,11,15]]},"reference":[{"key":"2025032412091471000_r1","volume-title":"Sequences of Proteins of Immunological Interest.","author":"Kabat","year":"1991"},{"key":"2025032412091471000_r2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1074-7613(00)00006-6","article-title":"Diversity in the CDR3 region of V(H) is sufficient for most antibody specificities","volume":"13","author":"Xu","year":"2000","journal-title":"Immunity"},{"key":"2025032412091471000_r3","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/0092-8674(89)90609-0","article-title":"Junctional sequences of T cell receptor gamma delta genes: implications for gamma delta T cell lineages and for a novel intermediate of V-(D)-J joining","volume":"59","author":"Lafaille","year":"1989","journal-title":"Cell"},{"key":"2025032412091471000_r4","doi-asserted-by":"crossref","first-page":"4118","DOI":"10.1073\/pnas.79.13.4118","article-title":"Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions","volume":"79","author":"Alt","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032412091471000_r5","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/302575a0","article-title":"Somatic generation of antibody diversity","volume":"302","author":"Tonegawa","year":"1983","journal-title":"Nature"},{"key":"2025032412091471000_r6","doi-asserted-by":"crossref","first-page":"7773","DOI":"10.4049\/jimmunol.174.12.7773","article-title":"Development of the expressed Ig CDR-H3 repertoire is marked by focusing of constraints in length, amino acid use, and charge that are first established in early B cell progenitors","volume":"174","author":"Ivanov","year":"2005","journal-title":"J. Immunol."},{"key":"2025032412091471000_r7","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.jmb.2003.10.007","article-title":"Expressed murine and human CDR-H3 intervals of equal length exhibit distinct repertoires that differ in their amino acid composition and predicted range of structures","volume":"334","author":"Zemlin","year":"2003","journal-title":"J. Mol. 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