{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:50:19Z","timestamp":1787223019337,"version":"build-2736575974"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Tissue inflammation is accompanied by the cytokine-mediated replacement of constitutive proteasomes by immunoproteasomes that finally leads to an optimized generation of MHC class I restricted epitopes for Ag presentation. The brain is considered an immunoprivileged organ, where both the special anatomy as well as active tolerance mechanisms repress the development of inflammatory responses and help to prevent immunopathological damage. We analyzed the immunoproteasome expression in the brain after an infection with lymphocytic choriomeningitis virus (LCMV) and could show that LCMV-infection of mice leads to the transcriptional induction of inducible proteasome subunits in the brain. However, compared with other organs, i.p. and even intracranial infection with LCMV only led to a faint expression of mature immunoproteasome in the brain and resulted in the accumulation of immunoproteasomal precursors. By immunohistology, we could identify microglia-like cells as the main producers of immunoproteasome, whereas in astrocytes immunoproteasome expression was almost exclusively restricted to nuclei. Neither the immunoproteasome subunits low molecular mass polypeptide 2 nor multicatalytic endopeptidase complex-like\u20131 were detected in neurons or oligodendrocytes. In vitro studies of IFN-\u03b3\u2013stimulated primary astrocytes suggested that the observed accumulation of immunoproteasomal precursor complexes takes place in this cell population. Functionally, the lack of immunoproteasomes protracted and lowered the severity of LCMV-induced meningitis in LMP7\u2212\/\u2212 mice suggesting a contribution of immunoproteasomes in microglia to exacerbate immunopathological damage. We postulate a posttranslationally regulated mechanism that prevents abundant and inappropriate immunoproteasome assembly in the brain and may contribute to the protection of poorly regenerating cells of the CNS from immunopathological destruction.<\/jats:p>","DOI":"10.4049\/jimmunol.1001517","type":"journal-article","created":{"date-parts":[[2010,9,30]],"date-time":"2010-09-30T03:40:16Z","timestamp":1285818016000},"page":"5549-5560","source":"Crossref","is-referenced-by-count":60,"title":["Reduced Immunoproteasome Formation and Accumulation of Immunoproteasomal Precursors in the Brains of Lymphocytic Choriomeningitis Virus-Infected Mice"],"prefix":"10.1093","volume":"185","author":[{"given":"Marcel","family":"Kremer","sequence":"first","affiliation":[{"name":"Division of Immunology, Department of Biology, Constance University , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Henn","sequence":"additional","affiliation":[{"name":"Doerenkamp-Zbinden Chair of in-vitro Toxicology and Biomedicine, Constance University , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Kolb","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Biology, Constance University , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Basler","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Biology, Constance University , Konstanz ,","place":["Germany"]},{"name":"Biotechnology Institute Thurgau, University of Constance , Kreuzlingen ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Moebius","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Biology, Constance University , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet","family":"Guillaume","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research-Brussels Branch, de Duve Institute, Universit\u00e9 catholique de Louvain , Brussels ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Leist","sequence":"additional","affiliation":[{"name":"Doerenkamp-Zbinden Chair of in-vitro Toxicology and Biomedicine, Constance University , Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet J","family":"Van den Eynde","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research-Brussels Branch, de Duve Institute, Universit\u00e9 catholique de Louvain , Brussels ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Groettrup","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Biology, Constance University , Konstanz ,","place":["Germany"]},{"name":"Biotechnology Institute Thurgau, University of Constance , Kreuzlingen ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,11,1]]},"reference":[{"key":"2025032412054311300_r1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1038\/nri2687","article-title":"Proteasomes in immune cells: more than peptide producers?","volume":"10","author":"Groettrup","year":"2010","journal-title":"Nat. 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