{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:45:13Z","timestamp":1758818713992,"version":"3.40.3"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Fucosyltransferase-IV and -VII double knockout (FtDKO) mice reveal profound impairment in T cell trafficking to lymph nodes (LNs) due to an inability to synthesize selectin ligands. We observed an increase in the proportion of memory\/effector (CD44high) T cells in LNs of FtDKO mice. We infected FtDKO mice with lymphocytic choriomeningitis virus to generate and track Ag-specific CD44highCD8 T cells in secondary lymphoid organs. Although frequencies were similar, total Ag-specific effector CD44highCD8 T cells were significantly reduced in LNs, but not blood, of FtDKO mice at day 8. In contrast, frequencies of Ag-specific memory CD44highCD8 T cells were up to 8-fold higher in LNs of FtDKO mice at day 60. Because wild-type mice treated with anti-CD62L treatment also showed increased frequencies of CD44high T cells in LNs, we hypothesized that memory T cells were preferentially retained in, or preferentially migrated to, FtDKO LNs. We analyzed T cell entry and egress in LNs using adoptive transfer of bone fide naive or memory T cells. Memory T cells were not retained longer in LNs compared with naive T cells; however, T cell exit slowed significantly as T cell numbers declined. Memory T cells were profoundly impaired in entering LNs of FtDKO mice; however, memory T cells exhibited greater homeostatic proliferation in FtDKO mice. These results suggest that memory T cells are enriched in LNs with T cell deficits by several mechanisms, including longer T cell retention and increased homeostatic proliferation.<\/jats:p>","DOI":"10.4049\/jimmunol.1001878","type":"journal-article","created":{"date-parts":[[2010,10,12]],"date-time":"2010-10-12T00:52:13Z","timestamp":1286844733000},"page":"5751-5761","source":"Crossref","is-referenced-by-count":12,"title":["Memory T Cells Are Enriched in Lymph Nodes of Selectin-Ligand\u2013Deficient Mice"],"prefix":"10.1093","volume":"185","author":[{"given":"John R","family":"Harp","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Tennessee , Knoxville, TN 37996"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A","family":"Gilchrist","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology, University of Tennessee , Knoxville, TN 37996"},{"name":"National Institute for Mathematical and Biological Synthesis, University of Tennessee , Knoxville, TN 37996"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thandi M","family":"Onami","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Tennessee , Knoxville, TN 37996"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,11,15]]},"reference":[{"key":"2025032412091248200_r1","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1084\/jem.194.7.953","article-title":"Migratory properties of naive, effector, and memory CD8(+) T cells","volume":"194","author":"Weninger","year":"2001","journal-title":"J. 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