{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:07:44Z","timestamp":1791194864373,"version":"4.1.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chicken CD4+CD25+ cells were characterized for mammalian regulatory T cells\u2019 suppressive and cytokine production properties. Anti-chicken CD25 mAb was produced in mice and conjugated with a fluorescent tag. The specificity of the Ab against chicken CD25 was confirmed by evaluating Con A-induced CD25 upregulation in thymocytes and by quantifying the CD25 mRNA content of positive and negative cells identified by anti-chicken CD25 Ab. The percentage of CD4+CD25+ cells, expressed as a percentage of CD4+ cells, in thymus and blood was \u223c3\u20137%, in spleen was 10%, and in cecal tonsil, lung, and bone marrow was \u223c15%. Bursa had no detectable CD4+CD25+ cells. CD25+ cells were mostly CD4+ in the thymus, whereas in every other organ studied, CD25+ cells were distributed between CD4+ and CD4\u2212 cells. Chicken thymic CD4+CD25+ cells did not proliferate in vitro in the absence of recombinant chicken IL-2 (rCIL-2). In the presence of rCIL-2, PMA plus ionomycin or Con A stimulated CD4+CD25+ cell proliferation, whereas anti-CD3 plus CD28 did not stimulate CD4+CD25+ cell proliferation. Naive CD4+CD25+ cells had 29-fold more IL-10 mRNA and 15-fold more TGF-\u03b2 mRNA than the naive CD4+CD25\u2212 cells. Naive CD4+CD25+ had no detectable IL-2 mRNA. Both naive and PMA plus ionomycin-stimulated thymic CD4+CD25+ cells suppressed naive T cell proliferation. The suppressive properties were partially contact dependent. Supplementing CD4+CD25+ cell coculture with rCIL-2 reversed the suppressive properties of CD4+CD25+ cells. Chicken CD4+CD25+ cells have suppressive properties similar to that of mammalian regulatory T cells.<\/jats:p>","DOI":"10.4049\/jimmunol.1002040","type":"journal-article","created":{"date-parts":[[2011,1,17]],"date-time":"2011-01-17T23:32:38Z","timestamp":1295307158000},"page":"1997-2002","source":"Crossref","is-referenced-by-count":124,"title":["Regulatory T Cell Properties of Chicken CD4+CD25+ Cells"],"prefix":"10.1093","volume":"186","author":[{"given":"Revathi","family":"Shanmugasundaram","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, Ohio Agricultural Research and Development Center , Wooster, OH 44691"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh K","family":"Selvaraj","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Ohio Agricultural Research and Development Center , Wooster, OH 44691"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,2,15]]},"reference":[{"key":"2025032411062869900_r1","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.cell.2008.05.009","article-title":"Regulatory T cells and immune tolerance","volume":"133","author":"Sakaguchi","year":"2008","journal-title":"Cell"},{"key":"2025032411062869900_r2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1128\/JVI.01768-07","article-title":"Natural regulatory T cells and persistent viral infection","volume":"82","author":"Li","year":"2008","journal-title":"J. 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