{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:29:32Z","timestamp":1791181772128,"version":"4.1.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Clinical use of antibiotics is based on their capacity to inhibit bacterial growth via bacteriostatic or bacteriocidal effects. In this article, we show that the aminoglycoside antibiotic neomycin, the cyclic lipopeptide antibiotic polymyxin B, and the cyclic peptide antibiotics gramicidin and tyrothricin can induce IL-1\u03b2 secretion in bone marrow dendritic cells and macrophages. LPS priming was required to trigger the transcription and translation of pro\u2013IL-1\u03b2 but was independent of TNFR or IL-1R signaling. All four antibiotics required the NLRP3 inflammasome, the adaptor ASC, and caspase-1 activation to secrete IL-1\u03b2, a process that depended on potassium efflux but was independent of P2X7 receptor. All four antibiotics induced neutrophil influx into the peritoneal cavity of mice, which required NLRP3 only in the case of polymyxin B. Together, certain antibiotics have the potential to directly activate innate immunity of the host.<\/jats:p>","DOI":"10.4049\/jimmunol.1002657","type":"journal-article","created":{"date-parts":[[2011,1,29]],"date-time":"2011-01-29T02:21:21Z","timestamp":1296267681000},"page":"2714-2718","source":"Crossref","is-referenced-by-count":47,"title":["Cutting Edge: Cyclic Polypeptide and Aminoglycoside Antibiotics Trigger IL-1\u03b2 Secretion by Activating the NLRP3 Inflammasome"],"prefix":"10.1093","volume":"186","author":[{"given":"Ramanjaneyulu","family":"Allam","sequence":"first","affiliation":[{"name":"Medizinische Poliklinik, Universit\u00e4t M\u00fcnchen , Munich ,","place":["Germany"]},{"name":"Department of Biochemistry, University of Lausanne , Epalinges ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murthy Narayana","family":"Darisipudi","sequence":"additional","affiliation":[{"name":"Medizinische Poliklinik, Universit\u00e4t M\u00fcnchen , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khader Valli","family":"Rupanagudi","sequence":"additional","affiliation":[{"name":"Medizinische Poliklinik, Universit\u00e4t M\u00fcnchen , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Lichtnekert","sequence":"additional","affiliation":[{"name":"Medizinische Poliklinik, Universit\u00e4t M\u00fcnchen , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurg","family":"Tschopp","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Lausanne , Epalinges ,","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Joachim","family":"Anders","sequence":"additional","affiliation":[{"name":"Medizinische Poliklinik, Universit\u00e4t M\u00fcnchen , Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,3,1]]},"reference":[{"key":"2025032411111481800_r1","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/ni.1863","article-title":"The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors","volume":"11","author":"Kawai","year":"2010","journal-title":"Nat. Immunol."},{"key":"2025032411111481800_r2","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/j.cell.2010.01.040","article-title":"The inflammasomes","volume":"140","author":"Schroder","year":"2010","journal-title":"Cell"},{"key":"2025032411111481800_r3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/S1074-7613(04)00046-9","article-title":"NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder","volume":"20","author":"Agostini","year":"2004","journal-title":"Immunity"},{"key":"2025032411111481800_r4","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nature02664","article-title":"Differential activation of the inflammasome by caspase-1 adaptors ASC and Ipaf","volume":"430","author":"Mariathasan","year":"2004","journal-title":"Nature"},{"key":"2025032411111481800_r5","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/nature07725","article-title":"AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC","volume":"458","author":"Hornung","year":"2009","journal-title":"Nature"},{"key":"2025032411111481800_r6","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1038\/ng1724","article-title":"Nalp1b controls mouse macrophage susceptibility to anthrax lethal toxin","volume":"38","author":"Boyden","year":"2006","journal-title":"Nat. Genet."},{"key":"2025032411111481800_r7","doi-asserted-by":"crossref","first-page":"e7446","DOI":"10.1371\/journal.pone.0007446","article-title":"Staphylococcus aureus alpha-hemolysin activates the NLRP3-inflammasome in human and mouse monocytic cells","volume":"4","author":"Craven","year":"2009","journal-title":"PLoS ONE"},{"key":"2025032411111481800_r8","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/j.cell.2006.07.033","article-title":"Caspase-1 activation of lipid metabolic pathways in response to bacterial pore-forming toxins promotes cell survival","volume":"126","author":"Gurcel","year":"2006","journal-title":"Cell"},{"key":"2025032411111481800_r9","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1038\/nature04515","article-title":"Cryopyrin activates the inflammasome in response to toxins and ATP","volume":"440","author":"Mariathasan","year":"2006","journal-title":"Nature"},{"key":"2025032411111481800_r10","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1002\/j.1460-2075.1995.tb07149.x","article-title":"Potassium-inhibited processing of IL-1 beta in human monocytes","volume":"14","author":"Walev","year":"1995","journal-title":"EMBO J."},{"key":"2025032411111481800_r11","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/nature04516","article-title":"Gout-associated uric acid crystals activate the NALP3 inflammasome","volume":"440","author":"Martinon","year":"2006","journal-title":"Nature"},{"key":"2025032411111481800_r12","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1074\/jbc.M006781200","article-title":"Altered cytokine production in mice lacking P2X(7) receptors","volume":"276","author":"Solle","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2025032411111481800_r13","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.1681\/ASN.2008101067","article-title":"Viral RNA and DNA trigger common antiviral responses in mesangial cells","volume":"20","author":"Allam","year":"2009","journal-title":"J. Am. Soc. Nephrol."},{"key":"2025032411111481800_r14","doi-asserted-by":"crossref","first-page":"792","DOI":"10.4049\/jimmunol.0900173","article-title":"Cutting edge: TNF-alpha mediates sensitization to ATP and silica via the NLRP3 inflammasome in the absence of microbial stimulation","volume":"183","author":"Franchi","year":"2009","journal-title":"J. Immunol."},{"key":"2025032411111481800_r15","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1146\/annurev.immunol.021908.132715","article-title":"The inflammasomes: guardians of the body","volume":"27","author":"Martinon","year":"2009","journal-title":"Annu. Rev. Immunol."},{"key":"2025032411111481800_r16","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/ni.1631","article-title":"Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization","volume":"9","author":"Hornung","year":"2008","journal-title":"Nat. Immunol."},{"key":"2025032411111481800_r17","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/ni.1831","article-title":"Thioredoxin-interacting protein links oxidative stress to inflammasome activation","volume":"11","author":"Zhou","year":"2010","journal-title":"Nat. Immunol."},{"key":"2025032411111481800_r18","doi-asserted-by":"crossref","first-page":"3821","DOI":"10.1007\/s00018-009-0129-9","article-title":"Gramicidin S and polymyxins: the revival of cationic cyclic peptide antibiotics","volume":"66","author":"Mogi","year":"2009","journal-title":"Cell. Mol. Life Sci."},{"key":"2025032411111481800_r19","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0005-2736(99)00207-2","article-title":"The interaction of the antimicrobial peptide gramicidin S with lipid bilayer model and biological membranes","volume":"1462","author":"Prenner","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"key":"2025032411111481800_r20","first-page":"675","article-title":"Antimicrobial agents\u2014Part II. The aminoglycosides: streptomycin, kanamycin, gentamicin, tobramycin, amikacin, neomycin","volume":"52","author":"Brewer","year":"1977","journal-title":"Mayo Clin. Proc."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/186\/5\/2714\/62474433\/1002657.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/186\/5\/2714\/62474433\/1002657.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T11:06:57Z","timestamp":1743160017000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/186\/5\/2714\/7990175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,1]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,3,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1002657","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2011,3]]},"published":{"date-parts":[[2011,3,1]]}}}