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In a novel murine model mimicking natural dermal infection, CCL2-dependent bone marrow (BM)-derived monocyte migration, differentiation into Ly6C+ dendritic cells (DC), and accumulation around dermal deposits of infected fibroblasts by day 1 postinfection were associated with increasing numbers of monocyte-derived TNF\/inducible NO synthase-producing DC by day 2 postinfection in draining auricular lymph nodes (ALN). Adoptive transfer demonstrated simultaneous migration of bone marrow-derived Ly6Clo monocytes to virus-infected dermis and ALN, where they first become Ly6Chi DC within 24 h and then Ly6Clo DC by 72 h. DC migration from the infected dermis to the ALN derived exclusively from Ly6Clo BM monocytes. This demonstrates that Ly6Chi and Ly6Clo BM-derived monocytes have different fates in vivo and suggests that BM may be a reservoir of preinflammatory monocytes for rapid deployment as inflammatory DC during virus infection.<\/jats:p>","DOI":"10.4049\/jimmunol.1002682","type":"journal-article","created":{"date-parts":[[2011,1,20]],"date-time":"2011-01-20T04:02:34Z","timestamp":1295496154000},"page":"2382-2396","source":"Crossref","is-referenced-by-count":30,"title":["Accelerated Dendritic Cell Differentiation from Migrating Ly6Clo Bone Marrow Monocytes in Early Dermal West Nile Virus Infection"],"prefix":"10.1093","volume":"186","author":[{"given":"Ariane M","family":"Davison","sequence":"first","affiliation":[{"name":"Discipline of Pathology, School of Medical Sciences, Bosch Institute, University of Sydney , Sydney, New South Wales 2006 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J C","family":"King","sequence":"additional","affiliation":[{"name":"Discipline of Pathology, School of Medical Sciences, Bosch Institute, University of Sydney , Sydney, New South Wales 2006 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,2,15]]},"reference":[{"key":"2025032411050451600_r1","doi-asserted-by":"crossref","first-page":"741","DOI":"10.4049\/jimmunol.167.2.741","article-title":"The dendritic cell populations of mouse lymph nodes","volume":"167","author":"Henri","year":"2001","journal-title":"J. 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