{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:27:14Z","timestamp":1787030834321,"version":"build-2736575974"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The environmental bacterium Burkholderia cenocepacia causes opportunistic lung infections in immunocompromised individuals, particularly in patients with cystic fibrosis. Infections in these patients are associated with exacerbated inflammation leading to rapid decay of lung function, and in some cases resulting in cepacia syndrome, which is characterized by a fatal acute necrotizing pneumonia and sepsis. B. cenocepacia can survive intracellularly in macrophages by altering the maturation of the phagosome, but very little is known on macrophage responses to the intracellular infection. In this study, we have examined the role of the PI3K\/Akt signaling pathway in B. cenocepacia-infected monocytes and macrophages. We show that PI3K\/Akt activity was required for NF-\u03baB activity and the secretion of proinflammatory cytokines during infection with B. cenocepacia. In contrast to previous observations in epithelial cells infected with other Gram-negative bacteria, Akt did not enhance I\u03baB kinase or NF-\u03baB p65 phosphorylation, but rather inhibited GSK3\u03b2, a negative regulator of NF-\u03baB transcriptional activity. This novel mechanism of modulation of NF-\u03baB activity may provide a unique therapeutic target for controlling excessive inflammation upon B. cenocepacia infection.<\/jats:p>","DOI":"10.4049\/jimmunol.1003034","type":"journal-article","created":{"date-parts":[[2011,6,22]],"date-time":"2011-06-22T23:13:04Z","timestamp":1308784384000},"page":"635-643","source":"Crossref","is-referenced-by-count":23,"title":["Akt-Mediated Proinflammatory Response of Mononuclear Phagocytes Infected with\n                    <i>Burkholderia cenocepacia<\/i>\n                    Occurs by a Novel GSK3\u03b2-Dependent, I\u03baB Kinase-Independent Mechanism"],"prefix":"10.1093","volume":"187","author":[{"given":"Thomas J","family":"Cremer","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, The Ohio State University Medical Center , Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prexy","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, The Ohio State University Medical Center , Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Cormet-Boyaka","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, The Ohio State University Medical Center , Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A","family":"Valvano","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Center for Human Immunology, University of Western Ontario , London, Ontario N6A 5C1 ,","place":["Canada"]},{"name":"Department of Medicine, Center for Human Immunology, University of Western Ontario , London, Ontario N6A 5C1 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan P","family":"Butchar","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, The Ohio State University Medical Center , Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susheela","family":"Tridandapani","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, The Ohio State University Medical Center , Columbus, OH 43210"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,7,15]]},"reference":[{"key":"2025032410212186100_r1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.2217\/17460913.1.2.145","article-title":"Infections by Burkholderia spp.: the psychodramatic life of an opportunistic pathogen","volume":"1","author":"Valvano","year":"2006","journal-title":"Future Microbiol."},{"key":"2025032410212186100_r2","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1099\/0022-1317-50-6-535","article-title":"Immunolocalisation of Burkholderia cepacia in the lungs of cystic fibrosis patients","volume":"50","author":"Sajjan","year":"2001","journal-title":"J. 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