{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T23:43:21Z","timestamp":1770594201518,"version":"3.49.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>For 15 y, \u03b1 B-crystallin (heat shock protein [Hsp] B5) has been labeled an autoantigen in multiple sclerosis (MS) based on humoral and cellular responses found in humans and animal models. However, there have been several scientific inconsistencies with this assignment, ranging from studies demonstrating small differences in anticrystallin responses between patients and healthy individuals to the inability of crystallin-specific T cells to induce symptoms of experimental allergic encephalomyelitis in animal models. Experiments in this article demonstrate that the putative anti-HspB5 Abs from 23 MS patients cross-react with 7 other members of the human small Hsp family and were equally present in normal plasma. Biolayer interferometry demonstrates that the binding was temperature dependent, and that the calculated K \u00a0a increased as the concentration of the sHsp decreased. These two patterns are characteristic of multiple binding sites with varying affinities, the composition of which changes with temperature, supporting the hypothesis that HspB5 bound the Ab and not the reverse. HspB5 also precipitated Ig heavy and L chains from sera from patients with MS. These results establish that small Hsps bind Igs with high affinity and refute much of the serological data used to assign \u03b1 B-crystallin as an autoantigen.<\/jats:p>","DOI":"10.4049\/jimmunol.1003934","type":"journal-article","created":{"date-parts":[[2011,2,26]],"date-time":"2011-02-26T05:53:36Z","timestamp":1298699616000},"page":"4263-4268","source":"Crossref","is-referenced-by-count":28,"title":["Chaperone Activity of \u03b1 B-Crystallin Is Responsible for Its Incorrect Assignment as an Autoantigen in Multiple Sclerosis"],"prefix":"10.1093","volume":"186","author":[{"given":"Jonathan B","family":"Rothbard","sequence":"first","affiliation":[{"name":"Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"},{"name":"Veterans Affairs Palo Alto Health Care System , Palo Alto, CA 94304"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orr","family":"Sharpe","sequence":"additional","affiliation":[{"name":"Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"},{"name":"Veterans Affairs Palo Alto Health Care System , Palo Alto, CA 94304"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J","family":"Strohman","sequence":"additional","affiliation":[{"name":"Program in Immunology, Department of Pediatrics, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Kurnellas","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth D","family":"Mellins","sequence":"additional","affiliation":[{"name":"Program in Immunology, Department of Pediatrics, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William H","family":"Robinson","sequence":"additional","affiliation":[{"name":"Division of Immunology and Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"},{"name":"Veterans Affairs Palo Alto Health Care System , Palo Alto, CA 94304"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence","family":"Steinman","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford University , Stanford, CA 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,4,1]]},"reference":[{"key":"2025032411174603500_r1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1038\/nri2548","article-title":"A molecular trio in relapse and remission in multiple sclerosis","volume":"9","author":"Steinman","year":"2009","journal-title":"Nat. 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