{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:16:40Z","timestamp":1762521400689,"version":"3.40.3"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Binding of immunoglobulin protein (BiP) is a major molecular chaperone localized in endoplasmic reticulum (ER). It has been demonstrated to interact with nascent Ig. However, contrary to other ER-resident heat shock proteins such as gp96, calreticulin, and ORP150, it is not clear whether tumor-derived BiP plays a role in inducing antitumor immunity. In this study, we show that the tumor-derived secreted form of BiP is capable of inducing antitumor CD8+ T cell responses. We constructed an ER-retention signal KDEL-deleted mutant of BiP cDNA and transfected it to tumor cells, which resulted in continuous secretion of tumor-derived BiP into the extracellular milieu. We show that this secreted BiP is taken up by bone marrow-derived dendritic cells, and thereafter BiP-associated Ag peptide is cross-presented in association with MHC class I molecules, resulting in elicitation of an Ag-specific CD8+ T cell response and antitumor effect. This strategy to boost antitumor immune responses shows that a tumor could be its own cellular vaccine via gene modification of the secretion of the tumor Ag\u2013BiP complex.<\/jats:p>","DOI":"10.4049\/jimmunol.1004048","type":"journal-article","created":{"date-parts":[[2011,2,22]],"date-time":"2011-02-22T03:08:51Z","timestamp":1298344131000},"page":"4325-4330","source":"Crossref","is-referenced-by-count":16,"title":["Tumor-Produced Secreted Form of Binding of Immunoglobulin Protein Elicits Antigen-Specific Tumor Immunity"],"prefix":"10.1093","volume":"186","author":[{"given":"Yasuaki","family":"Tamura","sequence":"first","affiliation":[{"name":"Department of Pathology, Sapporo Medical University School of Medicine , Sapporo 060-8556 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Hirohashi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sapporo Medical University School of Medicine , Sapporo 060-8556 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goro","family":"Kutomi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sapporo Medical University School of Medicine , Sapporo 060-8556 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuya","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sapporo Medical University School of Medicine , Sapporo 060-8556 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenjirou","family":"Kamiguchi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sapporo Medical University School of Medicine , Sapporo 060-8556 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Torigoe","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sapporo Medical University School of Medicine , Sapporo 060-8556 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sapporo Medical University School of Medicine , Sapporo 060-8556 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,4,1]]},"reference":[{"key":"2025032411162918700_r1","doi-asserted-by":"crossref","first-page":"3077","DOI":"10.1073\/pnas.91.8.3077","article-title":"Cellular requirements for tumor-specific immunity elicited by heat shock proteins: tumor rejection antigen gp96 primes CD8+ T cells in vivo","volume":"91","author":"Udono","year":"1994","journal-title":"Proc. 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Immunol."},{"key":"2025032411162918700_r6","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1126\/science.278.5335.117","article-title":"Immunotherapy of tumors with autologous tumor-derived heat shock protein preparations","volume":"278","author":"Tamura","year":"1997","journal-title":"Science"},{"key":"2025032411162918700_r7","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev.immunol.20.100301.064801","article-title":"Interaction of heat shock proteins with peptides and antigen presenting cells: chaperoning of the innate and adaptive immune responses","volume":"20","author":"Srivastava","year":"2002","journal-title":"Annu. Rev. 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