{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:33:00Z","timestamp":1791325980940,"version":"4.3.1"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Molluscum contagiosum virus (MCV) causes persistent neoplasms in healthy and immunocompromised people. Its ability to persist likely is due to its arsenal of viral immunoevasion proteins. For example, the MCV MC159 protein inhibits TNF-R1\u2013induced NF-\u03baB activation and apoptosis. The MC159 protein is a viral FLIP and, as such, possesses two tandem death effector domains (DEDs). We show in this article that, in human embryonic kidney 293 T cells, the expression of wild-type MC159 or a mutant MC159 protein containing the first DED (MC159 A) inhibited TNF-induced NF-\u03baB, or NF-\u03baB activated by PMA or MyD88 overexpression, whereas a mutant protein lacking the first DED (MC159 B) did not. We hypothesized that the MC159 protein targeted the I\u03baB kinase (IKK) complex to inhibit these diverse signaling events. Indeed, the MC159 protein, but not MC159 B, coimmunoprecipitated with IKK\u03b3. MC159 coimmunoprecipitated with IKK\u03b3 when using mouse embryonic fibroblasts that lack either IKK\u03b1 or IKK\u03b2, suggesting that the MC159 protein interacted directly with IKK\u03b3. MC159-IKK\u03b3 coimmunoprecipitations were detected during infection of cells with either MCV isolated from human lesions or with a recombinant MC159-expressing vaccinia virus. MC159 also interacts with TRAF2, a signaling molecule involved in NF-\u03baB activation. However, mutational analysis of MC159 failed to reveal a correlation between MC159\u2013TRAF2 interactions and MC159\u2019s inhibitory function. We propose that MC159\u2013IKK interactions, but not MC159\u2013TRAF2 interactions, are responsible for inhibiting NF-\u03baB activation.<\/jats:p>","DOI":"10.4049\/jimmunol.1100136","type":"journal-article","created":{"date-parts":[[2012,2,2]],"date-time":"2012-02-02T20:45:23Z","timestamp":1328215523000},"page":"2371-2379","source":"Crossref","is-referenced-by-count":35,"title":["The MC159 Protein from the Molluscum Contagiosum Poxvirus Inhibits NF-\u03baB Activation by Interacting with the I\u03baB Kinase Complex"],"prefix":"10.1093","volume":"188","author":[{"given":"Crystal M H","family":"Randall","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, University of Illinois , Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet A","family":"Jokela","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, University of Illinois , Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna L","family":"Shisler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Medicine, University of Illinois , Urbana, IL 61801"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,3,1]]},"reference":[{"key":"2025032409562022800_r1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1006\/viro.1999.9649","article-title":"Characterization of early gene transcripts of molluscum contagiosum virus","volume":"257","author":"Bugert","year":"1999","journal-title":"Virology"},{"key":"2025032409562022800_r2","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1126\/science.273.5276.813","article-title":"Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes","volume":"273","author":"Senkevich","year":"1996","journal-title":"Science"},{"key":"2025032409562022800_r3","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1006\/viro.1997.8607","article-title":"The genome of molluscum contagiosum virus: analysis and comparison with other poxviruses","volume":"233","author":"Senkevich","year":"1997","journal-title":"Virology"},{"issue":"3","key":"2025032409562022800_r4","doi-asserted-by":"crossref","first-page":"S12","DOI":"10.1067\/S0002-9378(03)00793-2","article-title":"Molluscum contagiosum: the importance of early diagnosis and treatment","volume":"189","author":"Tyring","year":"2003","journal-title":"Am. 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