{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:21:00Z","timestamp":1790234460279,"version":"4.1.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The activation of the BCR, which initiates B cell activation, is triggered by Ag-induced self-aggregation and clustering of receptors at the cell surface. Although Ag-induced actin reorganization is known to be involved in BCR clustering in response to membrane-associated Ag, the underlying mechanism that links actin reorganization to BCR activation remains unknown. In this study, we show that both the stimulatory Bruton\u2019s tyrosine kinase (Btk) and the inhibitory SHIP-1 are required for efficient BCR self-aggregation. In Btk-deficient B cells, the magnitude of BCR aggregation into clusters and B cell spreading in response to an Ag-tethered lipid bilayer is drastically reduced, compared with BCR aggregation observed in wild-type B cells. In SHIP-1\u2212\/\u2212 B cells, although surface BCRs aggregate into microclusters, the centripetal movement and growth of BCR clusters are inhibited, and B cell spreading is increased. The persistent BCR microclusters in SHIP-1\u2212\/\u2212 B cells exhibit higher levels of signaling than merged BCR clusters. In contrast to the inhibition of actin remodeling in Btk-deficient B cells, actin polymerization, F-actin accumulation, and Wiskott\u2013Aldrich symptom protein phosphorylation are enhanced in SHIP-1\u2212\/\u2212 B cells in a Btk-dependent manner. Thus, a balance between positive and negative signaling regulates the spatiotemporal organization of the BCR at the cell surface by controlling actin remodeling, which potentially regulates the signal transduction of the BCR. This study suggests a novel feedback loop between BCR signaling and the actin cytoskeleton.<\/jats:p>","DOI":"10.4049\/jimmunol.1100157","type":"journal-article","created":{"date-parts":[[2011,5,28]],"date-time":"2011-05-28T03:06:40Z","timestamp":1306552000000},"page":"230-239","source":"Crossref","is-referenced-by-count":77,"title":["A Balance of Bruton\u2019s Tyrosine Kinase and SHIP Activation Regulates B Cell Receptor Cluster Formation by Controlling Actin Remodeling"],"prefix":"10.1093","volume":"187","author":[{"given":"Chaohong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland , College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland , College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"King Lam","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Maryland , College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Grooman","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Maryland , College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Bolland","sequence":"additional","affiliation":[{"name":"Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Rockville, MD 20852"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpita","family":"Upadhyaya","sequence":"additional","affiliation":[{"name":"Department of Physics, University of Maryland , College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxia","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland , College Park, MD 20742"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,7,1]]},"reference":[{"key":"2025032410202540500_r1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.molimm.2004.04.008","article-title":"B cell antigen receptor signaling 101","volume":"41","author":"Dal Porto","year":"2004","journal-title":"Mol. 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