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In this study, we demonstrated that Ag-specific CD8+ T cells that migrate to the mouse brain following a systemic Listeria infection do not display markers reminiscent of long-term memory cells. In contrast to spleen and other nonlymphoid organs, none of the CD8+ T cells in the brain reverted to a memory phenotype, and all of the cells were gradually eliminated. These nonmemory phenotype CD8+ T cells were found primarily within the choroid plexus, as well as in the cerebrospinal fluid-filled spaces. Entry of these CD8+ T cells into the brain was governed primarily by CD49d\/VCAM-1, with the majority of entry occurring in the first week postinfection. When CD8+ T cells were injected directly into the brain parenchyma, cells that remained in the brain retained a highly activated (CD69hi) phenotype and were gradually lost, whereas those that migrated out to the spleen were CD69low and persisted long-term. These results revealed a mechanism of time-bound immune surveillance to the brain by CD8+ T cells that do not reside in the parenchyma.<\/jats:p>","DOI":"10.4049\/jimmunol.1100695","type":"journal-article","created":{"date-parts":[[2011,6,30]],"date-time":"2011-06-30T04:36:32Z","timestamp":1309408592000},"page":"1192-1200","source":"Crossref","is-referenced-by-count":34,"title":["CD8+ T Cells Primed in the Periphery Provide Time-Bound Immune-Surveillance to the Central Nervous System"],"prefix":"10.1093","volume":"187","author":[{"given":"Kevin G","family":"Young","sequence":"first","affiliation":[{"name":"National Research Council of Canada-Institute for Biological Sciences , Ottawa, Ontario K1A 0R6 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"MacLean","sequence":"additional","affiliation":[{"name":"National Research Council of Canada-Institute for Biological Sciences , Ottawa, Ontario K1A 0R6 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Dudani","sequence":"additional","affiliation":[{"name":"National Research Council of Canada-Institute for Biological Sciences , Ottawa, Ontario K1A 0R6 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshmi","family":"Krishnan","sequence":"additional","affiliation":[{"name":"National Research Council of Canada-Institute for Biological Sciences , Ottawa, Ontario K1A 0R6 ,","place":["Canada"]},{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa , Ottawa, Ontario K1H 8M5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subash","family":"Sad","sequence":"additional","affiliation":[{"name":"National Research Council of Canada-Institute for Biological Sciences , Ottawa, Ontario K1A 0R6 ,","place":["Canada"]},{"name":"Department of Biochemistry, Microbiology and Immunology, University of Ottawa , Ottawa, Ontario K1H 8M5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,8,1]]},"reference":[{"key":"2025032410233738500_r1","doi-asserted-by":"crossref","first-page":"6360","DOI":"10.4049\/jimmunol.0803798","article-title":"Critical roles of NK and CD8+ T cells in central nervous system listeriosis","volume":"182","author":"Hayashi","year":"2009","journal-title":"J. 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