{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:16:30Z","timestamp":1792012590291,"version":"4.4.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>B cell development is exquisitely sensitive to location within specialized niches in the bone marrow and spleen. Location within these niches is carefully orchestrated through chemotactic and adhesive cues. In this article, we demonstrate the requirement for the actin-bundling protein L-plastin (LPL) in B cell motility toward the chemokines CXCL12 and CXCL13 and the lipid chemoattractant sphingosine-1-phosphate, which guide normal B cell development. Impaired motility of B cells in LPL\u2212\/\u2212 mice correlated with diminished splenic maturation of B cells, with a moderate (40%) loss of follicular B cells and a profound (&amp;gt;80%) loss of marginal zone B cells. Entry of LPL\u2212\/\u2212 B cells into the lymph nodes and bone marrow of mice was also impaired. Furthermore, LPL was required for the integrin-mediated enhancement of Transwell migration but was dispensable for integrin-mediated lymphocyte adhesion. These results suggest that LPL may participate in signaling that enables lymphocyte transmigration. In support of this hypothesis, the phosphorylation of Pyk-2, a tyrosine kinase that integrates chemotactic and adhesive cues, is diminished in LPL\u2212\/\u2212 B cells stimulated with chemokine. Finally, a well-characterized role of marginal zone B cells is the generation of a rapid humoral response to polysaccharide Ags. LPL\u2212\/\u2212 mice exhibited a defective Ab response to Streptococcus pneumoniae, indicating a functional consequence of defective marginal zone B cell development in LPL\u2212\/\u2212 mice.<\/jats:p>","DOI":"10.4049\/jimmunol.1101033","type":"journal-article","created":{"date-parts":[[2011,8,11]],"date-time":"2011-08-11T04:35:15Z","timestamp":1313037315000},"page":"3015-3025","source":"Crossref","is-referenced-by-count":46,"title":["The Actin-Bundling Protein L-Plastin Is Essential for Marginal Zone B Cell Development"],"prefix":"10.1093","volume":"187","author":[{"given":"Elizabeth M","family":"Todd","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine , St. Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren E","family":"Deady","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine , St. Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon Celeste","family":"Morley","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics, Washington University School of Medicine , St. Louis, MO 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,9,15]]},"reference":[{"key":"2025032410335064300_r1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/nri1268","article-title":"Role of the cytoskeleton during leukocyte responses","volume":"4","author":"Vicente-Manzanares","year":"2004","journal-title":"Nat. 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