{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:50:22Z","timestamp":1787223022510,"version":"build-2736575974"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The replacement of the catalytically active proteasome subunits \u03b21, \u03b22, and \u03b25 by the immunoproteasome subunits low molecular mass polypeptide (LMP) 2 (\u03b21i), multicatalytic endopeptidase complex-like\u20131 (MECL-1) (\u03b22i), and LMP7 (\u03b25i) is required for the production of numerous class I ligands. Hitherto, investigation of the immunoproteasome was confined to the analysis of mice deficient for one or two immunosubunits. In this study, we characterized LMP2\u2212\/\u2212\/MECL-1\u2212\/\u2212 double-deficient mice and used the well-defined LMP7-selective inhibitor ONX 0914 in these mice to generate mice lacking the activity of all immunoproteasome subunits. LMP2\u2212\/\u2212\/MECL-1\u2212\/\u2212 double-deficient mice had strongly reduced numbers of CD8+ T cells in the spleen. Nevertheless, infection with the lymphocytic choriomeningits virus induced a normal cytotoxic T cell response in these mice, although the T cell response to several class I epitopes was altered. Treatment of LMP2\u2212\/\u2212\/MECL-1\u2212\/\u2212 double-deficient mice with the LMP7-selective inhibitor ONX 0914 elicited a strong CTL response in lymphocytic choriomeningitis virus-infected mice. Thereby, the TCD8+ response to nucleoprotein 205\u2013212, which is barely detectable in LMP2\u2212\/\u2212\/MECL-1\u2212\/\u2212 double-deficient mice, could be reverted to normal levels by LMP7 inhibition. Additional experiments could demonstrate that the increased CTL response to the nucleoprotein 205\u2013212 in mice lacking functional immunoproteasome is due to an altered class I presentation of this epitope. Taken together, to our knowledge, this is the first study investigating viral infection in mice lacking activity of all three immunoproteasome subunits.<\/jats:p>","DOI":"10.4049\/jimmunol.1101064","type":"journal-article","created":{"date-parts":[[2011,10,20]],"date-time":"2011-10-20T04:49:41Z","timestamp":1319086181000},"page":"5548-5557","source":"Crossref","is-referenced-by-count":47,"title":["The Antiviral Immune Response in Mice Devoid of Immunoproteasome Activity"],"prefix":"10.1093","volume":"187","author":[{"given":"Michael","family":"Basler","sequence":"first","affiliation":[{"name":"Biotechnology Institute Thurgau at Constance University , CH-8280 Kreuzlingen ,","place":["Switzerland"]},{"name":"Division of Immunology, Department of Biology, University of Constance , D-78457 Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Beck","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Biology, University of Constance , D-78457 Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J","family":"Kirk","sequence":"additional","affiliation":[{"name":"Onyx Pharmaceuticals , South San Francisco, CA 94080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Groettrup","sequence":"additional","affiliation":[{"name":"Biotechnology Institute Thurgau at Constance University , CH-8280 Kreuzlingen ,","place":["Switzerland"]},{"name":"Division of Immunology, Department of Biology, University of Constance , D-78457 Konstanz ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,12,1]]},"reference":[{"key":"2025032410505142400_r1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/0167-5699(96)10051-7","article-title":"Peptide antigen production by the proteasome: complexity provides efficiency","volume":"17","author":"Groettrup","year":"1996","journal-title":"Immunol. 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