{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:43:34Z","timestamp":1770835414816,"version":"3.50.1"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Protein kinase C (PKC)-\u03b8 is involved in T cell activation via regulating the avidity of the \u03b22 integrin LFA-1 in the immunological synapse. LFA-1 also mediates leukocyte adhesion. To investigate the role of PKC-\u03b8 in neutrophil adhesion, we performed intravital microscopy in cremaster venules of mice reconstituted with bone marrow from LysM-GFP+ (wild-type [WT]) and PKC-\u03b8 gene-deficient (Prkcq\u2212\/\u2212) mice. Following stimulation with CXCL1, both WT and Prkcq\u2212\/\u2212 cells became adherent. Although most WT neutrophils remained adherent for at least 180 s, 50% of Prkcq\u2212\/\u2212 neutrophils were detached after 105 s and most by 180 s. Upon CXCL1 injection, rolling of all WT neutrophils stopped for 90 s, but rolling of Prkcq\u2212\/\u2212 neutrophils started 30 s after CXCL1 stimulation. A similar neutrophil adhesion defect was seen in vitro, and spreading of Prkcq\u2212\/\u2212 neutrophils was delayed. Prkcq\u2212\/\u2212 neutrophil recruitment was impaired in fMLP-induced transmigration into the cremaster muscle, thioglycollate-induced peritonitis, and LPS-induced lung injury. We conclude that PKC-\u03b8 mediates integrin-dependent neutrophil functions and is required to sustain neutrophil adhesion in postcapillary venules in vivo. These findings suggest that the role of PKC-\u03b8 in outside\u2013in signaling following engagement of neutrophil integrins is relevant for inflammation in vivo.<\/jats:p>","DOI":"10.4049\/jimmunol.1101651","type":"journal-article","created":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T05:22:43Z","timestamp":1331184163000},"page":"4043-4051","source":"Crossref","is-referenced-by-count":24,"title":["Protein Kinase C-\u03b8 Is Required for Murine Neutrophil Recruitment and Adhesion Strengthening under Flow"],"prefix":"10.1093","volume":"188","author":[{"given":"Anna","family":"Bertram","sequence":"first","affiliation":[{"name":"Department of Nephrology and Hypertensiology, Hannover Medical School , 30625 Hannover ,","place":["Germany"]},{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibylle","family":"von Vietinghoff","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertensiology, Hannover Medical School , 30625 Hannover ,","place":["Germany"]},{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"de Pablo","sequence":"additional","affiliation":[{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Haller","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertensiology, Hannover Medical School , 30625 Hannover ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelli","family":"Shushakova","sequence":"additional","affiliation":[{"name":"Department of Nephrology and Hypertensiology, Hannover Medical School , 30625 Hannover ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Ley","sequence":"additional","affiliation":[{"name":"Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology , La Jolla, CA 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,4,15]]},"reference":[{"key":"2025032410013697300_r1","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1111\/j.1582-4934.2009.00811.x","article-title":"Leukocyte-endothelial interactions in inflammation","volume":"13","author":"Langer","year":"2009","journal-title":"J. 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