{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:05:11Z","timestamp":1791198311323,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The critical role of Langerhans cells (LC) in contact hypersensitivity (CHS) was recently questioned in studies using different LC-depletion mouse models. On one hand, inducible ablation of LC led to diminished ear swelling, suggesting functional redundancy between LC and (Langerin+) dermal dendritic cells (DC). On the other hand, constitutive or acute depletion of LC resulted in an enhanced reaction, supporting a regulatory role of LC in CHS. To address this controversy by conditional gene targeting, we generated Langerin-Cre knockin mice. Breeding these mice to a Cre-reporter strain demonstrated robust and specific DNA recombination in LC, as well as other Langerin+ tissue DC. In agreement with the vital requirement of TGF-\u03b2 signaling for LC development, crossing Langerin-Cre to mice homozygous for a loxP-flanked TGF-\u03b2R1 allele resulted in permanent LC deficiency, whereas the homeostasis of dermal Langerin+ DC was unaffected. In the absence of LC, induction of CHS in these Langerin+ DC-specific TGF-\u03b2R1\u2013deficient mice elicited decreased ear swelling compared with controls. This novel approach provided further evidence against a regulatory function of LC in CHS. Moreover, these Langerin-Cre mice represent a unique and powerful tool to dissect the role and molecular control of Langerin+ DC populations beyond LC.<\/jats:p>","DOI":"10.4049\/jimmunol.1101880","type":"journal-article","created":{"date-parts":[[2011,10,13]],"date-time":"2011-10-13T05:34:25Z","timestamp":1318484065000},"page":"5069-5076","source":"Crossref","is-referenced-by-count":80,"title":["Conditional Deletion of TGF-\u03b2R1 Using Langerin-Cre Mice Results in Langerhans Cell Deficiency and Reduced Contact Hypersensitivity"],"prefix":"10.1093","volume":"187","author":[{"given":"Sonja P","family":"Zahner","sequence":"first","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , 3015 GE Rotterdam ,","place":["The Netherlands"]},{"name":"Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam , 1105 AZ Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junda M","family":"Kel","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , 3015 GE Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cerithsa A E","family":"Martina","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Histology, Academic Medical Center, University of Amsterdam , 1105 AZ Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inge","family":"Brouwers-Haspels","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , 3015 GE Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marian A","family":"van Roon","sequence":"additional","affiliation":[{"name":"Animal Research Institute, Facility for Genetically Modified Mice, Academic Medical Center , 1105 AZ Amsterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bj\u00f6rn E","family":"Clausen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus University Medical Center , 3015 GE Rotterdam ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,11,15]]},"reference":[{"key":"2025032410462651900_r1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.0105-2896.2009.00886.x","article-title":"Langerhans cells and more: langerin-expressing dendritic cell subsets in the skin","volume":"234","author":"Romani","year":"2010","journal-title":"Immunol. 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