{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:43:06Z","timestamp":1789987386681,"version":"4.0.1"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The four dengue virus (DENV) serotypes cause dengue fever and dengue hemorrhagic fever\/dengue shock syndrome. Although severe disease has been associated with heterotypic secondary DENV infection, most secondary DENV infections are asymptomatic or result in classic DF. The role of cross-reactive immunity in mediating cross-protection against secondary heterotypic DENV infection is not well understood. DENV infection of IFN-\u03b1\/\u03b2 and IFN-\u03b3 receptor-deficient (AG129) mice reproduces key features of human disease. We previously demonstrated a role in cross-protection for pre-existing cross-reactive Abs, maintained by long-lived plasma cells. In this study, we use a sequential infection model, infecting AG129 mice with DENV-1, followed by DENV-2 6\u20138 wk later. We find that increased DENV-specific avidity during acute secondary heterotypic infection is mediated by cross-reactive memory B cells, as evidenced by increased numbers of DENV-1\u2013specific cells by ELISPOT and higher avidity against DENV-1 of supernatants from polyclonally stimulated splenocytes isolated from mice experiencing secondary DENV-2 infection. However, increased DENV-specific avidity is not associated with increased DENV-specific neutralization, which appears to be mediated by naive B cells. Adoptive transfer of DENV-1\u2013immune B and T cells into naive mice prior to secondary DENV-2 infection delayed mortality. Mice depleted of T cells developed signs of disease, but recovered after secondary DENV infection. Overall, we found that protective cross-reactive Abs are secreted by both long-lived plasma cells and memory B cells and that both cross-reactive B cells and T cells provide protection against a secondary heterotypic DENV infection. Understanding the protective immunity that develops naturally against DENV infection may help design future vaccines.<\/jats:p>","DOI":"10.4049\/jimmunol.1102124","type":"journal-article","created":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T05:55:46Z","timestamp":1322805346000},"page":"404-416","source":"Crossref","is-referenced-by-count":92,"title":["Protection from Secondary Dengue Virus Infection in a Mouse Model Reveals the Role of Serotype Cross-Reactive B and T Cells"],"prefix":"10.1093","volume":"188","author":[{"given":"Simona","family":"Zompi","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California , Berkeley, Berkeley, CA 94720-7354"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian H","family":"Santich","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California , Berkeley, Berkeley, CA 94720-7354"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P Robert","family":"Beatty","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California , Berkeley, Berkeley, CA 94720-7354"},{"name":"Department of Molecular and Cellular Biology, University of California , Berkeley, Berkeley, CA 94720-3200"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Harris","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California , Berkeley, Berkeley, CA 94720-7354"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,1,1]]},"reference":[{"key":"2025032409425738000_r1","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S0966-842X(01)02288-0","article-title":"Epidemic dengue\/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century","volume":"10","author":"Gubler","year":"2002","journal-title":"Trends Microbiol."},{"key":"2025032409425738000_r2","doi-asserted-by":"crossref","first-page":"179","DOI":"10.4269\/ajtmh.1990.42.179","article-title":"Dengue hemorrhagic fever in Cuba, 1981: a retrospective seroepidemiologic study","volume":"42","author":"Guzm\u00e1n","year":"1990","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2025032409425738000_r3","doi-asserted-by":"crossref","first-page":"30","DOI":"10.4269\/ajtmh.1952.1.30","article-title":"Research on dengue during World War II","volume":"1","author":"Sabin","year":"1952","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2025032409425738000_r4","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1093\/oxfordjournals.aje.a113932","article-title":"Risk factors in dengue shock syndrome: a prospective epidemiologic study in Rayong, Thailand. I. The 1980 outbreak","volume":"120","author":"Sangkawibha","year":"1984","journal-title":"Am. J. Epidemiol."},{"key":"2025032409425738000_r5","doi-asserted-by":"crossref","first-page":"566","DOI":"10.4269\/ajtmh.1997.56.566","article-title":"Risk factors in dengue shock syndrome","volume":"56","author":"Thein","year":"1997","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2025032409425738000_r6","first-page":"83","article-title":"Cellular immunology of sequential dengue virus infection and its role in disease pathogenesis","volume":"338","author":"Rothman","year":"2010","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"2025032409425738000_r7","doi-asserted-by":"crossref","first-page":"e1000790","DOI":"10.1371\/journal.ppat.1000790","article-title":"Lethal antibody enhancement of dengue disease in mice is prevented by Fc modification","volume":"6","author":"Balsitis","year":"2010","journal-title":"PLoS Pathog."},{"key":"2025032409425738000_r8","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1093\/aje\/152.9.793","article-title":"Epidemiologic studies on dengue in Santiago de Cuba, 1997","volume":"152","author":"Guzm\u00e1n","year":"2000","journal-title":"Am. J. Epidemiol."},{"key":"2025032409425738000_r9","doi-asserted-by":"crossref","first-page":"411","DOI":"10.4269\/ajtmh.1988.38.411","article-title":"Evidence that maternal dengue antibodies are important in the development of dengue hemorrhagic fever in infants","volume":"38","author":"Kliks","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2025032409425738000_r10","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1038\/nm887","article-title":"Original antigenic sin and apoptosis in the pathogenesis of dengue hemorrhagic fever","volume":"9","author":"Mongkolsapaya","year":"2003","journal-title":"Nat. Med."},{"key":"2025032409425738000_r11","doi-asserted-by":"crossref","first-page":"10081","DOI":"10.1128\/JVI.00330-07","article-title":"Differential functional avidity of dengue virus-specific T-cell clones for variant peptides representing heterologous and previously encountered serotypes","volume":"81","author":"Imrie","year":"2007","journal-title":"J. Virol."},{"key":"2025032409425738000_r12","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.4049\/jimmunol.175.4.2676","article-title":"Altered cytokine responses of dengue-specific CD4+ T cells to heterologous serotypes","volume":"175","author":"Mangada","year":"2005","journal-title":"J. Immunol."},{"key":"2025032409425738000_r13","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1086\/526790","article-title":"Cross-reactive memory CD8(+) T cells alter the immune response to heterologous secondary dengue virus infections in mice in a sequence-specific manner","volume":"197","author":"Beaumier","year":"2008","journal-title":"J. Infect. Dis."},{"key":"2025032409425738000_r14","doi-asserted-by":"crossref","first-page":"444","DOI":"10.4269\/ajtmh.1989.40.444","article-title":"Antibody-dependent enhancement of dengue virus growth in human monocytes as a risk factor for dengue hemorrhagic fever","volume":"40","author":"Kliks","year":"1989","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2025032409425738000_r15","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/S0140-6736(02)09522-3","article-title":"Effect of dengue-1 antibodies on American dengue-2 viral infection and dengue haemorrhagic fever","volume":"360","author":"Kochel","year":"2002","journal-title":"Lancet"},{"key":"2025032409425738000_r16","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.virol.2008.08.008","article-title":"Antibodies play a greater role than immune cells in heterologous protection against secondary dengue virus infection in a mouse model","volume":"380","author":"Kyle","year":"2008","journal-title":"Virology"},{"key":"2025032409425738000_r17","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1146\/annurev.immunol.23.021704.115732","article-title":"Antigen-specific memory B cell development","volume":"23","author":"McHeyzer-Williams","year":"2005","journal-title":"Annu. Rev. Immunol."},{"key":"2025032409425738000_r18","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1002\/(SICI)1521-4141(199904)29:04<1406::AID-IMMU1406>3.0.CO;2-P","article-title":"Correlation analysis between frequencies of circulating antigen-specific IgG-bearing memory B cells and serum titers of antigen-specific IgG","volume":"29","author":"Leyendeckers","year":"1999","journal-title":"Eur. J. Immunol."},{"key":"2025032409425738000_r19","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1600-065X.2010.00912.x","article-title":"Mechanisms that determine plasma cell lifespan and the duration of humoral immunity","volume":"236","author":"Amanna","year":"2010","journal-title":"Immunol. Rev."},{"key":"2025032409425738000_r20","doi-asserted-by":"crossref","first-page":"4802","DOI":"10.1073\/pnas.0800555105","article-title":"Maintenance of the plasma cell pool is independent of memory B cells","volume":"105","author":"Ahuja","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032409425738000_r21","doi-asserted-by":"crossref","first-page":"E12","DOI":"10.1111\/j.1749-6632.2009.05057.x","article-title":"A mouse model for studying dengue virus pathogenesis and immune response","volume":"1171","author":"Williams","year":"2009","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2025032409425738000_r22","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1086\/518007","article-title":"Dengue virus infects macrophages and dendritic cells in a mouse model of infection","volume":"195","author":"Kyle","year":"2007","journal-title":"J. Infect. Dis."},{"key":"2025032409425738000_r23","doi-asserted-by":"crossref","first-page":"10208","DOI":"10.1128\/JVI.00062-06","article-title":"Murine model for dengue virus-induced lethal disease with increased vascular permeability","volume":"80","author":"Shresta","year":"2006","journal-title":"J. Virol."},{"key":"2025032409425738000_r24","doi-asserted-by":"crossref","first-page":"416","DOI":"10.4269\/ajtmh.2009.80.416","article-title":"Tropism of dengue virus in mice and humans defined by viral nonstructural protein 3-specific immunostaining","volume":"80","author":"Balsitis","year":"2009","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2025032409425738000_r25","doi-asserted-by":"crossref","first-page":"8411","DOI":"10.1128\/JVI.00611-08","article-title":"A mouse-passaged dengue virus strain with reduced affinity for heparan sulfate causes severe disease in mice by establishing increased systemic viral loads","volume":"82","author":"Prestwood","year":"2008","journal-title":"J. Virol."},{"key":"2025032409425738000_r26","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1080\/15476910701385687","article-title":"Interlaboratory study of the primary antibody response to sheep red blood cells in outbred rodents following exposure to cyclophosphamide or dexamethasone","volume":"4","author":"Loveless","year":"2007","journal-title":"J. Immunotoxicol."},{"key":"2025032409425738000_r27","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1620\/tjem.162.147","article-title":"Differential effects of a single dose of cyclophosphamide on T cell subsets of the thymus and spleen in mice: flow cytofluorometry analysis","volume":"162","author":"Miyauchi","year":"1990","journal-title":"Tohoku J. Exp. Med."},{"key":"2025032409425738000_r28","doi-asserted-by":"crossref","first-page":"4865","DOI":"10.4049\/jimmunol.0801974","article-title":"A protective role for dengue virus-specific CD8+ T cells","volume":"182","author":"Yauch","year":"2009","journal-title":"J. Immunol."},{"key":"2025032409425738000_r29","doi-asserted-by":"crossref","first-page":"5405","DOI":"10.4049\/jimmunol.1001709","article-title":"CD4+ T cells are not required for the induction of dengue virus-specific CD8+ T cell or antibody responses but contribute to protection after vaccination","volume":"185","author":"Yauch","year":"2010","journal-title":"J. Immunol."},{"key":"2025032409425738000_r30","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.virol.2005.08.034","article-title":"Inhibition of dengue virus translation and RNA synthesis by a morpholino oligomer targeted to the top of the terminal 3\u2032 stem-loop structure","volume":"344","author":"Holden","year":"2006","journal-title":"Virology"},{"key":"2025032409425738000_r31","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.virol.2003.09.048","article-title":"Early activation of natural killer and B cells in response to primary dengue virus infection in A\/J mice","volume":"319","author":"Shresta","year":"2004","journal-title":"Virology"},{"key":"2025032409425738000_r32","doi-asserted-by":"crossref","first-page":"3777","DOI":"10.1128\/JCM.00827-07","article-title":"Comparison of plaque- and flow cytometry-based methods for measuring dengue virus neutralization","volume":"45","author":"Kraus","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"2025032409425738000_r33","doi-asserted-by":"crossref","first-page":"6077","DOI":"10.1016\/j.vaccine.2008.09.009","article-title":"Adverse event reports following yellow fever vaccination","volume":"26","author":"Lindsey","year":"2008","journal-title":"Vaccine"},{"key":"2025032409425738000_r34","doi-asserted-by":"crossref","DOI":"10.1016\/B978-0-12-442702-0.50020-0","volume-title":"Induction of Antibody Formation in Mouse Bone Marrow.","author":"Benner","year":"1981"},{"key":"2025032409425738000_r35","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.jim.2003.12.015","article-title":"Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system","volume":"286","author":"Crotty","year":"2004","journal-title":"J. Immunol. Methods"},{"key":"2025032409425738000_r36","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.virol.2005.10.030","article-title":"A rapid and quantitative assay for measuring antibody-mediated neutralization of West Nile virus infection","volume":"346","author":"Pierson","year":"2006","journal-title":"Virology"},{"key":"2025032409425738000_r37","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nm.1894","article-title":"Lack of antibody affinity maturation due to poor Toll-like receptor stimulation leads to enhanced respiratory syncytial virus disease","volume":"15","author":"Delgado","year":"2009","journal-title":"Nat. Med."},{"key":"2025032409425738000_r38","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1038\/nm918","article-title":"A role for nonprotective complement-fixing antibodies with low avidity for measles virus in atypical measles","volume":"9","author":"Polack","year":"2003","journal-title":"Nat. Med."},{"key":"2025032409425738000_r39","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/S0952-7915(98)80162-3","article-title":"Long-lived plasma cells: a mechanism for maintaining persistent antibody production","volume":"10","author":"Slifka","year":"1998","journal-title":"Curr. Opin. Immunol."},{"key":"2025032409425738000_r40","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/S1074-7613(00)80541-5","article-title":"Humoral immunity due to long-lived plasma cells","volume":"8","author":"Slifka","year":"1998","journal-title":"Immunity"},{"key":"2025032409425738000_r41","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1038\/sj.emboj.7601009","article-title":"Progressively impaired proteasomal capacity during terminal plasma cell differentiation","volume":"25","author":"Cenci","year":"2006","journal-title":"EMBO J."},{"key":"2025032409425738000_r42","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.0105-2896.2006.00403.x","article-title":"Understanding and making use of human memory B cells","volume":"211","author":"Lanzavecchia","year":"2006","journal-title":"Immunol. Rev."},{"key":"2025032409425738000_r43","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.4049\/jimmunol.0902708","article-title":"IFN-gamma receptor signaling regulates memory CD8+ T cell differentiation","volume":"184","author":"Sercan","year":"2010","journal-title":"J. Immunol."},{"key":"2025032409425738000_r44","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.virol.2007.05.042","article-title":"Characterization of an antigenic site that contains a dominant, type-specific neutralization determinant on the envelope protein domain III (ED3) of dengue 2 virus","volume":"366","author":"Gromowski","year":"2007","journal-title":"Virology"},{"key":"2025032409425738000_r45","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.chom.2010.01.004","article-title":"Enhanced infection of liver sinusoidal endothelial cells in a mouse model of antibody-induced severe dengue disease","volume":"7","author":"Zellweger","year":"2010","journal-title":"Cell Host Microbe"},{"key":"2025032409425738000_r46","first-page":"301","article-title":"Immune enhancement of viral infection","volume":"31","author":"Halstead","year":"1982","journal-title":"Prog. Allergy"},{"key":"2025032409425738000_r47","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1084\/jem.146.1.201","article-title":"Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody","volume":"146","author":"Halstead","year":"1977","journal-title":"J. Exp. Med."},{"key":"2025032409425738000_r48","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/265739a0","article-title":"Antibody-enhanced dengue virus infection in primate leukocytes","volume":"265","author":"Halstead","year":"1977","journal-title":"Nature"},{"key":"2025032409425738000_r49","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0065-2776(01)77018-6","article-title":"The antiviral activity of antibodies in vitro and in vivo","volume":"77","author":"Parren","year":"2001","journal-title":"Adv. Immunol."},{"key":"2025032409425738000_r50","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.chom.2008.08.004","article-title":"Structural insights into the mechanisms of antibody-mediated neutralization of flavivirus infection: implications for vaccine development","volume":"4","author":"Pierson","year":"2008","journal-title":"Cell Host Microbe"},{"key":"2025032409425738000_r51","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1038\/nri891","article-title":"Antibodies, viruses and vaccines","volume":"2","author":"Burton","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"2025032409425738000_r52","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.virol.2010.12.020","article-title":"Antibody-mediated neutralization of flaviviruses: a reductionist view","volume":"411","author":"Dowd","year":"2011","journal-title":"Virology"},{"key":"2025032409425738000_r53","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1128\/JCM.42.4.1782-1784.2004","article-title":"Use of an immunoglobulin G avidity test to discriminate between primary and secondary dengue virus infections","volume":"42","author":"de Souza","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"2025032409425738000_r54","doi-asserted-by":"crossref","first-page":"5784","DOI":"10.1128\/JCM.43.11.5784-5786.2005","article-title":"Use of four dengue virus antigens for determination of dengue immune status by enzyme-linked immunosorbent assay of immunoglobulin G avidity","volume":"43","author":"Matheus","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"2025032409425738000_r55","doi-asserted-by":"crossref","first-page":"2793","DOI":"10.1128\/JCM.43.6.2793-2797.2005","article-title":"Discrimination between primary and secondary dengue virus infection by an immunoglobulin G avidity test using a single acute-phase serum sample","volume":"43","author":"Matheus","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"2025032409425738000_r56","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1128\/JVI.01826-10","article-title":"An in-depth analysis of original antigenic sin in dengue virus infection","volume":"85","author":"Midgley","year":"2011","journal-title":"J. Virol."},{"key":"2025032409425738000_r57","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1093\/emboj\/16.11.2996","article-title":"The extent of affinity maturation differs between the memory and antibody-forming cell compartments in the primary immune response","volume":"16","author":"Smith","year":"1997","journal-title":"EMBO J."},{"key":"2025032409425738000_r58","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1084\/jem.20030091","article-title":"Activation of virus-specific memory B cells in the absence of T cell help","volume":"199","author":"Hebeis","year":"2004","journal-title":"J. Exp. Med."},{"key":"2025032409425738000_r59","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1002\/art.11181","article-title":"Serologic changes following B lymphocyte depletion therapy for rheumatoid arthritis","volume":"48","author":"Cambridge","year":"2003","journal-title":"Arthritis Rheum."},{"key":"2025032409425738000_r60","first-page":"225","article-title":"The dengue group of viruses and its family relationships","volume":"14","author":"Sabin","year":"1950","journal-title":"Bacteriol. Rev."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/188\/1\/404\/61615750\/1102124.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/188\/1\/404\/61615750\/1102124.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T07:25:07Z","timestamp":1743060307000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/188\/1\/404\/7978174"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,1]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1102124","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.14262991.15775105","asserted-by":"object"}]},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2012,1]]},"published":{"date-parts":[[2012,1,1]]}}}