{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:53:16Z","timestamp":1790110396368,"version":"4.0.1"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dengue virus infection can lead to life-threatening dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS) in patients. Abnormal activation of the coagulation and fibrinolysis system is one of the hallmarks associated with DHF\/DSS patients. However, the mechanisms that cause pathology in DHF\/DSS patients are still unclear. Because conversion of plasminogen (Plg) to plasmin (Plm) is the first step in the activation of fibrinolysis, Abs against Plg found in DHF\/DSS patients may be important. Therefore, to investigate the specificity, function, and possible origin of these Abs, we generated several Plg cross-reactive mAbs from DENV-immunized mice. An IgG mAb, 6H11, which recognizes an epitope associated with a dengue envelope protein, demonstrated a high level of cross-reactivity with Plg. The 6H11 Ab was further characterized with regard to its effect on Plg activation. Using Plm-specific chromogenic substrate S-2251, we found that mAb 6H11 demonstrated serine protease activity and could convert Plg directly to Plm. The serine protease activity of mAb 6H11 was further confirmed using serine protease chromogenic substrate S-2288. In addition, we found several Plg cross-reactive mAbs that could enhance urokinase-induced Plg activation. Lastly, mAb 6H11 could induce Plm activity and increase the level of D-dimer (a fibrin degradation product) in both human and mouse platelet-poor plasma. Taken together, these data suggest DENV-induced Plg cross-reactive Abs may enhance Plg conversion to Plm, which would be expected to contribute to hyperfibrinolysis in DHF\/DSS patients.<\/jats:p>","DOI":"10.4049\/jimmunol.1102218","type":"journal-article","created":{"date-parts":[[2011,11,12]],"date-time":"2011-11-12T08:25:37Z","timestamp":1321086337000},"page":"6483-6490","source":"Crossref","is-referenced-by-count":48,"title":["Dengue Virus-Induced Autoantibodies Bind to Plasminogen and Enhance Its Activation"],"prefix":"10.1093","volume":"187","author":[{"given":"Yung-Chun","family":"Chuang","sequence":"first","affiliation":[{"name":"Institute of Basic Medical Sciences, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]},{"name":"Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan-Yao","family":"Lei","sequence":"additional","affiliation":[{"name":"Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]},{"name":"Department of Microbiology and Immunology, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee-Shin","family":"Lin","sequence":"additional","affiliation":[{"name":"Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]},{"name":"Department of Microbiology and Immunology, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiao-Sheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]},{"name":"Department of Microbiology and Immunology, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua-Lin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trai-Ming","family":"Yeh","sequence":"additional","affiliation":[{"name":"Center of Infectious Disease and Signaling Research, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]},{"name":"Department of Medical Laboratory Science and Biotechnology, Medical College, National Cheng Kung University , Tainan ,","place":["Taiwan 701"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,12,15]]},"reference":[{"key":"2025032410540923800_r1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev.micro.62.081307.163005","article-title":"Global spread and persistence of dengue","volume":"62","author":"Kyle","year":"2008","journal-title":"Annu. 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