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Diacylglycerol (DAG) produced after TCR stimulation functions as a secondary messenger and mediates the signaling to Ras-MEK-Erk and NF-\u03baB pathways in T cells. DAG kinases (DGKs) convert DAG into phosphatidic acid, resulting in termination of DAG signaling. In this study, we demonstrate that DAG metabolism by DGKs can serve a crucial function in viral clearance upon lymphocytic choriomeningitis virus infection. Ag-specific CD8+ T cells from DGK\u03b1\u2212\/\u2212 and DGK\u03b6\u2212\/\u2212 mice show enhanced expansion and increased cytokine production after lymphocytic choriomeningitis virus infection, yet DGK-deficient memory CD8+ T cells exhibit impaired expansion after rechallenge. Thus, DGK activity plays opposing roles in the expansion of CD8+ T cells during the primary and memory phases of the immune response, whereas consistently inhibiting antiviral cytokine production.<\/jats:p>","DOI":"10.4049\/jimmunol.1102265","type":"journal-article","created":{"date-parts":[[2012,1,24]],"date-time":"2012-01-24T07:26:51Z","timestamp":1327390011000},"page":"2111-2117","source":"Crossref","is-referenced-by-count":42,"title":["Differential Regulation of Primary and Memory CD8 T Cell Immune Responses by Diacylglycerol Kinases"],"prefix":"10.1093","volume":"188","author":[{"given":"Jinwook","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F","family":"O\u2019Brien","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University Medical Center , Durham, NC 27710"},{"name":"Department of Immunology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M","family":"Grayson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Wake Forest University School of Medicine , Winston-Salem, NC 27157"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Ping","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Duke University Medical Center , Durham, NC 27710"},{"name":"Department of Immunology, Duke University Medical Center , Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,3,1]]},"reference":[{"key":"2025032409531118800_r1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1146\/annurev.immunol.25.022106.141548","article-title":"Effector and memory CTL differentiation","volume":"25","author":"Williams","year":"2007","journal-title":"Annu. 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