{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T14:28:53Z","timestamp":1778768933830,"version":"3.51.4"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Enhancement of stem cell Ag-1 (Sca-1) expression by myeloid precursors promotes the granulopoietic response to bacterial infection. However, the underlying mechanisms remain unclear. ERK pathway activation strongly enhances proliferation of hematopoietic progenitor cells. In this study, we investigated the role of Sca-1 in promoting ERK-dependent myeloid lineage proliferation and the effects of alcohol on this process. Thirty minutes after i.p. injection of alcohol, mice received i.v. challenge with 5 \u00d7 107 \u00a0Escherichia coli for 8 or 24 h. A subset of mice received i.v. BrdU injection 20 h after challenge. Bacteremia increased Sca-1 expression, ERK activation, and proliferation of myeloid and granulopoietic precursors. Alcohol administration suppressed this response and impaired granulocyte production. Sca-1 expression positively correlated with ERK activation and cell cycling, but negatively correlated with myeloperoxidase content in granulopoietic precursors. Alcohol intoxication suppressed ERK activation in granulopoietic precursors and proliferation of these cells during bacteremia. Granulopoietic precursors in Sca-1\u2212\/\u2212 mice failed to activate ERK signaling and could not increase granulomacrophagic CFU activity following bacteremia. These data indicate that Sca-1 expression promotes ERK-dependent myeloid cell proliferation during bacteremia. Suppression of this response could represent an underlying mechanism for developing myelosuppression in alcohol-abusing hosts with severe bacterial infection.<\/jats:p>","DOI":"10.4049\/jimmunol.1102395","type":"journal-article","created":{"date-parts":[[2012,1,12]],"date-time":"2012-01-12T06:17:32Z","timestamp":1326349052000},"page":"1961-1969","source":"Crossref","is-referenced-by-count":23,"title":["Alcohol Impairs the Myeloid Proliferative Response to Bacteremia in Mice by Inhibiting the Stem Cell Antigen-1\/ERK Pathway"],"prefix":"10.1093","volume":"188","author":[{"given":"John Nicholas","family":"Melvan","sequence":"first","affiliation":[{"name":"Department of Physiology, Louisiana State University Health Sciences Center , New Orleans, LA 70112"},{"name":"Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center , New Orleans, LA 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W","family":"Siggins","sequence":"additional","affiliation":[{"name":"Department of Physiology, Louisiana State University Health Sciences Center , New Orleans, LA 70112"},{"name":"Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center , New Orleans, LA 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William L","family":"Stanford","sequence":"additional","affiliation":[{"name":"Sprott Centre for Stem Cell Research, Ottawa Hospital Research Institute , Ottawa, Ontario K1H 8L6 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie","family":"Porretta","sequence":"additional","affiliation":[{"name":"Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center , New Orleans, LA 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Physiology, Louisiana State University Health Sciences Center , New Orleans, LA 70112"},{"name":"Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center , New Orleans, LA 70112"},{"name":"Section of Pulmonary\/Critical Care Medicine, Department of Medicine, Louisiana State University Health Sciences Center , New Orleans, LA 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory J","family":"Bagby","sequence":"additional","affiliation":[{"name":"Department of Physiology, Louisiana State University Health Sciences Center , New Orleans, LA 70112"},{"name":"Comprehensive Alcohol Research Center, Louisiana State University Health Sciences Center , New Orleans, LA 70112"},{"name":"Section of Pulmonary\/Critical Care Medicine, Department of Medicine, Louisiana State University Health Sciences Center , New Orleans, LA 70112"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Surgery, College of Human Medicine, Michigan State University , East Lansing, MI 48824"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,2,15]]},"reference":[{"key":"2025032409522458500_r1","first-page":"L587","article-title":"Polymorphonuclear leukocyte transit times in bone marrow during streptococcal pneumonia","volume":"271","author":"Terashima","year":"1996","journal-title":"Am. 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