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G5PR is upregulated in activated B cells, in Ki67-negative centrocytes at germinal centers (GCs), and in purified B220+Fas+GL7+ mature GC B cells following Ag immunization. G5PR rescues transformed B cells from BCR-mediated activation-induced cell death by suppression of late-phase JNK activation. In G5PR-transgenic (G5PRTg) mice, G5PR overexpression leads to an augmented generation of GC B cells via an increase in non-Ag\u2013specific B cells and a consequent reduction in the proportion of Ag-specific B cells and high-affinity Ab production after immunization with nitrophenyl-conjugated chicken \u03b3-globulin. G5PR overexpression impaired the affinity\u2013maturation of Ag-specific B cells, presumably by diluting the numbers of high-affinity B cells. However, aged nonimmunized female G5PRTg mice showed an increase in the numbers of peritoneal B-1a cells and the generation of autoantibodies. G5PR overexpression did not affect the proliferation of B-1a and B-2 cells but rescued B-1a cells from activation-induced cell death in vitro. G5PR might play a pivotal role in B cell selection not only for B-2 cells but also for B-1 cells in peripheral lymphoid organs.<\/jats:p>","DOI":"10.4049\/jimmunol.1102774","type":"journal-article","created":{"date-parts":[[2012,7,3]],"date-time":"2012-07-03T02:43:09Z","timestamp":1341283389000},"page":"1193-1201","source":"Crossref","is-referenced-by-count":12,"title":["Transgenic Overexpression of G5PR That Is Normally Augmented in Centrocytes Impairs the Enrichment of High-Affinity Antigen-Specific B Cells, Increases Peritoneal B-1a Cells, and Induces Autoimmunity in Aged Female Mice"],"prefix":"10.1093","volume":"189","author":[{"given":"Masahiro","family":"Kitabatake","sequence":"first","affiliation":[{"name":"Department of Immunology, Graduate School of Life Sciences, Kumamoto University , Kumamoto 860-8556,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teppei","family":"Toda","sequence":"additional","affiliation":[{"name":"Department of Immunology, Graduate School of Life Sciences, Kumamoto University , Kumamoto 860-8556,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Kuwahara","sequence":"additional","affiliation":[{"name":"Department of Immunology, Graduate School of Life Sciences, Kumamoto University , Kumamoto 860-8556,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideya","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Department of Immunology, Graduate School of Life Sciences, Kumamoto University , Kumamoto 860-8556,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mareki","family":"Ohtsuji","sequence":"additional","affiliation":[{"name":"Department of Pathology, Juntendo University Graduate School of Medicine , Bunkyo-ku, Tokyo 113-8341,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromichi","family":"Tsurui","sequence":"additional","affiliation":[{"name":"Department of Pathology, Juntendo University Graduate School of Medicine , Bunkyo-ku, Tokyo 113-8341,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiko","family":"Hirose","sequence":"additional","affiliation":[{"name":"Department of Pathology, Juntendo University Graduate School of Medicine , Bunkyo-ku, Tokyo 113-8341,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Sakaguchi","sequence":"additional","affiliation":[{"name":"Department of Immunology, Graduate School of Life Sciences, Kumamoto University , Kumamoto 860-8556,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,8,1]]},"reference":[{"key":"2025032409213887700_r1","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1146\/annurev.iy.12.040194.001001","article-title":"Germinal centers","volume":"12","author":"MacLennan","year":"1994","journal-title":"Annu. 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