{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:12:42Z","timestamp":1791353562939,"version":"4.3.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Brucella abortus is a Gram-negative bacterium causing brucellosis. Although B. abortus is known to infect via the oral route, the entry site in the gastrointestinal tract has been unclear. We found that B. abortus was selectively internalized by microfold cells (M cells), a subset of epithelial cells specialized for mucosal Ag uptake. During this process, colocalization of cellular prion protein (PrPC) and B. abortus was evident on the apical surface as well as in subapical vacuolar structures in M cells. Internalization of B. abortus by M cells of PrPC-deficient (Prnp\u2212\/\u2212) mice was greatly reduced compared with that in wild-type mice. Furthermore, an oral infection study revealed that translocation of B. abortus into the Peyer\u2019s patch was significantly lower in Prnp\u2212\/\u2212 than in wild-type mice. These observations suggest that orally infected B. abortus invades the host through M cells by using PrPC on the apical surface of M cells as an uptake receptor.<\/jats:p>","DOI":"10.4049\/jimmunol.1103332","type":"journal-article","created":{"date-parts":[[2012,7,7]],"date-time":"2012-07-07T02:05:43Z","timestamp":1341626743000},"page":"1540-1544","source":"Crossref","is-referenced-by-count":87,"title":["Cutting Edge:\n                    <i>Brucella abortus<\/i>\n                    Exploits a Cellular Prion Protein on Intestinal M Cells as an Invasive Receptor"],"prefix":"10.1093","volume":"189","author":[{"given":"Gaku","family":"Nakato","sequence":"first","affiliation":[{"name":"Laboratory for Epithelial Immunobiology, Research Center for Allergy and Immunology, RIKEN , Kanagawa 230-0045,","place":["Japan"]},{"name":"Department of Supramolecular Biology, Graduate School of Nanobiosciences, Yokohama City University , Kanagawa 230-0045,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Hase","sequence":"additional","affiliation":[{"name":"Laboratory for Epithelial Immunobiology, Research Center for Allergy and Immunology, RIKEN , Kanagawa 230-0045,","place":["Japan"]},{"name":"Department of Supramolecular Biology, Graduate School of Nanobiosciences, Yokohama City University , Kanagawa 230-0045,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michio","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases , Tokyo 162-8640,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanobu","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases , Tokyo 162-8640,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Ato","sequence":"additional","affiliation":[{"name":"Department of Immunology, National Institute of Infectious Diseases , Tokyo 162-8640,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misaho","family":"Hanazato","sequence":"additional","affiliation":[{"name":"Laboratory for Epithelial Immunobiology, Research Center for Allergy and Immunology, RIKEN , Kanagawa 230-0045,","place":["Japan"]},{"name":"Department of Supramolecular Biology, Graduate School of Nanobiosciences, Yokohama City University , Kanagawa 230-0045,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Tobiume","sequence":"additional","affiliation":[{"name":"Department of Pathology, National Institute of Infectious Diseases , Tokyo 162-8640,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motohiro","family":"Horiuchi","sequence":"additional","affiliation":[{"name":"Laboratory of Prion Diseases, Graduate School of Veterinary Medicine, Hokkaido University , Hokkaido 060-0818,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuichiro","family":"Atarashi","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences , Nagasaki 852-8523,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyuki","family":"Nishida","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences , Nagasaki 852-8523,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahisa","family":"Watarai","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Agriculture, Yamaguchi University , Yamaguchi 753-8515,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Imaoka","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, National Institute of Infectious Diseases , Tokyo 162-8640,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ohno","sequence":"additional","affiliation":[{"name":"Laboratory for Epithelial Immunobiology, Research Center for Allergy and Immunology, RIKEN , Kanagawa 230-0045,","place":["Japan"]},{"name":"Department of Supramolecular Biology, Graduate School of Nanobiosciences, Yokohama City University , Kanagawa 230-0045,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,8,15]]},"reference":[{"key":"2025032409223150300_r1","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1038\/ni1101-1004","article-title":"Collaboration of epithelial cells with organized mucosal lymphoid tissues","volume":"2","author":"Neutra","year":"2001","journal-title":"Nat. Immunol."},{"key":"2025032409223150300_r2","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/aja.1001360406","article-title":"Pinocytosis by epithelium associated with lymphoid follicles in the bursa of Fabricius, appendix, and Peyer\u2019s patches. An electron microscopic study","volume":"136","author":"Bockman","year":"1973","journal-title":"Am. J. Anat."},{"key":"2025032409223150300_r3","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/S0016-5085(77)80254-0","article-title":"Sequential uptake of horseradish peroxidase by lymphoid follicle epithelium of Peyer\u2019s patches in the normal unobstructed mouse intestine: an ultrastructural study","volume":"72","author":"Owen","year":"1977","journal-title":"Gastroenterology"},{"key":"2025032409223150300_r4","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1006\/smim.1999.0175","article-title":"M cells as ports of entry for enteroinvasive pathogens: mechanisms of interaction, consequences for the disease process","volume":"11","author":"Sansonetti","year":"1999","journal-title":"Semin. Immunol."},{"key":"2025032409223150300_r5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1574-6968.2001.tb10594.x","article-title":"Glycosylphosphatidylinositol-anchored receptor-mediated bacterial endocytosis","volume":"197","author":"Shin","year":"2001","journal-title":"FEMS Microbiol. Lett."},{"key":"2025032409223150300_r6","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1128\/IAI.66.3.1237-1243.1998","article-title":"M-cell surface beta1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse Peyer\u2019s patch M cells","volume":"66","author":"Clark","year":"1998","journal-title":"Infect. Immun."},{"key":"2025032409223150300_r7","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nature08529","article-title":"Uptake through glycoprotein 2 of FimH(+) bacteria by M cells initiates mucosal immune response","volume":"462","author":"Hase","year":"2009","journal-title":"Nature"},{"key":"2025032409223150300_r8","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1093\/dnares\/dsp013","article-title":"New approach for m-cell-specific molecules screening by comprehensive transcriptome analysis","volume":"16","author":"Nakato","year":"2009","journal-title":"DNA Res."},{"key":"2025032409223150300_r9","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0378-1135(02)00214-6","article-title":"Brucella intracellular life: from invasion to intracellular replication","volume":"90","author":"Gorvel","year":"2002","journal-title":"Vet. Microbiol."},{"key":"2025032409223150300_r10","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1006\/mpat.2002.0531","article-title":"Membrane sorting during swimming internalization of Brucella is required for phagosome trafficking decisions","volume":"33","author":"Kim","year":"2002","journal-title":"Microb. Pathog."},{"key":"2025032409223150300_r11","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1084\/jem.20030088","article-title":"Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum","volume":"198","author":"Celli","year":"2003","journal-title":"J. Exp. Med."},{"key":"2025032409223150300_r12","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1046\/j.1462-5822.2001.00102.x","article-title":"Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole","volume":"3","author":"Comerci","year":"2001","journal-title":"Cell. Microbiol."},{"key":"2025032409223150300_r13","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1084\/jem.20021980","article-title":"Cellular prion protein promotes Brucella infection into macrophages","volume":"198","author":"Watarai","year":"2003","journal-title":"J. Exp. Med."},{"key":"2025032409223150300_r14","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1038\/380528a0","article-title":"Loss of cerebellar Purkinje cells in aged mice homozygous for a disrupted PrP gene","volume":"380","author":"Sakaguchi","year":"1996","journal-title":"Nature"},{"key":"2025032409223150300_r15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/22241","article-title":"Prions prevent neuronal cell-line death","volume":"400","author":"Kuwahara","year":"1999","journal-title":"Nature"},{"key":"2025032409223150300_r16","doi-asserted-by":"crossref","first-page":"137","DOI":"10.7883\/yoken.JJID.2007.137","article-title":"Simultaneous detection of the genus Brucella by combinatorial PCR","volume":"60","author":"Imaoka","year":"2007","journal-title":"Jpn. J. Infect. Dis."},{"key":"2025032409223150300_r17","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1046\/j.1462-5822.2002.00195.x","article-title":"Modulation of Brucella-induced macropinocytosis by lipid rafts mediates intracellular replication","volume":"4","author":"Watarai","year":"2002","journal-title":"Cell. Microbiol."},{"key":"2025032409223150300_r18","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.virol.2003.10.026","article-title":"Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies","volume":"320","author":"Kim","year":"2004","journal-title":"Virology"},{"key":"2025032409223150300_r19","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1126\/science.6801762","article-title":"Novel proteinaceous infectious particles cause scrapie","volume":"216","author":"Prusiner","year":"1982","journal-title":"Science"},{"key":"2025032409223150300_r20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2249.2006.03194.x","article-title":"The role of the cellular prion protein in the immune system","volume":"146","author":"Isaacs","year":"2006","journal-title":"Clin. Exp. Immunol."},{"key":"2025032409223150300_r21","doi-asserted-by":"crossref","first-page":"6229","DOI":"10.1128\/IAI.73.10.6229-6236.2005","article-title":"Absence of evidence for the participation of the macrophage cellular prion protein in infection with Brucella suis","volume":"73","author":"Fontes","year":"2005","journal-title":"Infect. Immun."},{"key":"2025032409223150300_r22","doi-asserted-by":"crossref","first-page":"4724","DOI":"10.1128\/jvi.70.7.4724-4728.1996","article-title":"Prion protein PrPc interacts with molecular chaperones of the Hsp60 family","volume":"70","author":"Edenhofer","year":"1996","journal-title":"J. Virol."},{"key":"2025032409223150300_r23","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1007\/s00018-011-0847-7","article-title":"Allosteric function and dysfunction of the prion protein","volume":"69","author":"Linden","year":"2012","journal-title":"Cell. Mol. Life Sci."},{"key":"2025032409223150300_r24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0167-5699(90)90050-J","article-title":"Heat shock proteins and the immune response","volume":"11","author":"Kaufmann","year":"1990","journal-title":"Immunol. Today"},{"key":"2025032409223150300_r25","first-page":"58","article-title":"Surface-associated heat shock proteins of Legionella pneumophila and Helicobacter pylori: roles in pathogenesis and immunity","volume":"7","author":"Hoffman","year":"1999","journal-title":"Infect. Dis. Obstet. Gynecol."},{"key":"2025032409223150300_r26","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1128\/IAI.66.3.1252-1257.1998","article-title":"GroEL heat shock protein of Haemophilus ducreyi: association with cell surface and capacity to bind to eukaryotic cells","volume":"66","author":"Frisk","year":"1998","journal-title":"Infect. Immun."},{"key":"2025032409223150300_r27","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1128\/iai.60.8.3072-3078.1992","article-title":"A 66-kilodalton heat shock protein of Salmonella typhimurium is responsible for binding of the bacterium to intestinal mucus","volume":"60","author":"Ensgraber","year":"1992","journal-title":"Infect. Immun."},{"key":"2025032409223150300_r28","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1099\/00221287-147-1-87","article-title":"GroEL (Hsp60) of Clostridium difficile is involved in cell adherence","volume":"147","author":"Hennequin","year":"2001","journal-title":"Microbiology"},{"key":"2025032409223150300_r29","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1093\/intimm\/dxm008","article-title":"Essential role of Peyer\u2019s patches in the development of Helicobacter-induced gastritis","volume":"19","author":"Kiriya","year":"2007","journal-title":"Int. Immunol."},{"key":"2025032409223150300_r30","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1038\/39376","article-title":"Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic","volume":"389","author":"Baorto","year":"1997","journal-title":"Nature"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/189\/4\/1540\/61714789\/1103332.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/189\/4\/1540\/61714789\/1103332.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T01:32:34Z","timestamp":1743039154000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/189\/4\/1540\/7993672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,8,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1103332","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2012,8]]},"published":{"date-parts":[[2012,8]]}}}