{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:31:21Z","timestamp":1791322281326,"version":"4.3.1"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>V\u03b39V\u03b42 T cells are attractive candidates for antileukemic activity. The analysis of V\u03b39V\u03b42 T cells in newly diagnosed acute myeloid leukemia (AML) patients revealed that their absolute cell numbers were normal in the blood as well as in the bone marrow but showed a striking imbalance in the differentiation subsets, with preponderance of the effector memory population. This unusual phenotype was restored after removal of leukemic cells in patients, which reached complete remission after chemotherapy, suggesting that leukemic cells might be involved in the alteration of \u03b3\u03b4 T cell development in AML. Accordingly, coculture between AML cells and V\u03b39V\u03b42 T cells induced selection of effector cells. In accordance with their effector memory status, in vitro proliferation of V\u03b39V\u03b42 T cells was reduced compared with normal controls. Nevertheless, V\u03b39V\u03b42 T cells efficiently killed autologous AML blasts via the perforin\/granzyme pathway. The ligands for DNAM-1 were expressed by AML cells. We showed that killing of AML blasts was TCR and DNAM-1 dependent. Using a xenotransplantation murine model, we showed that V\u03b39V\u03b42 T cells homed to the bone marrow in close proximity of engrafted leukemic cells and enhanced survival. These data demonstrate that V\u03b39V\u03b42 T cells are endowed with the ability to interact with and eradicate AML blasts both in vitro and in a mouse model. Collectively, our data revealed that V\u03b39V\u03b42 T cells have a potent antileukemic activity provided that optimal activation is achieved, such as with synthetic TCR agonists. This study enhances the interest of these cells for therapeutic purposes such as AML treatment.<\/jats:p>","DOI":"10.4049\/jimmunol.1103710","type":"journal-article","created":{"date-parts":[[2012,3,31]],"date-time":"2012-03-31T05:12:54Z","timestamp":1333170774000},"page":"4701-4708","source":"Crossref","is-referenced-by-count":124,"title":["Human V\u03b39V\u03b42 T Cells Specifically Recognize and Kill Acute Myeloid Leukemic Blasts"],"prefix":"10.1093","volume":"188","author":[{"given":"Julie","family":"Gertner-Dardenne","sequence":"first","affiliation":[{"name":"Institut Paoli-Calmettes, Laboratoire d\u2019Immunologie des Tumeurs , Marseille F-13009 ,","place":["France"]},{"name":"INSERM, Unit\u00e9 891, Centre de Recherche en Canc\u00e9rologie de Marseille, Immunit\u00e9 et Cancers , Marseille F-13009 ,","place":["France"]},{"name":"Institut Paoli-Calmettes, Plateforme d'Immunomonitoring en Canc\u00e9rologie , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remy","family":"Castellano","sequence":"additional","affiliation":[{"name":"INSERM, Unit\u00e9 891, Centre de Recherche en Canc\u00e9rologie de Marseille, Institut Paoli-Calmettes, TrGET Plateforme d'Essais Pr\u00e9cliniques , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Mamessier","sequence":"additional","affiliation":[{"name":"Institut Paoli-Calmettes, Laboratoire d\u2019Immunologie des Tumeurs , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Slaveia","family":"Garbit","sequence":"additional","affiliation":[{"name":"INSERM, Unit\u00e9 891, Centre de Recherche en Canc\u00e9rologie de Marseille, Institut Paoli-Calmettes, TrGET Plateforme d'Essais Pr\u00e9cliniques , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elo\u00efse","family":"Kochbati","sequence":"additional","affiliation":[{"name":"Institut Paoli-Calmettes, Laboratoire d\u2019Immunologie des Tumeurs , Marseille F-13009 ,","place":["France"]},{"name":"Institut Paoli-Calmettes, Plateforme d'Immunomonitoring en Canc\u00e9rologie , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Etienne","sequence":"additional","affiliation":[{"name":"Institut Paoli-Calmettes, Service d\u2019H\u00e9matologie , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aude","family":"Charbonnier","sequence":"additional","affiliation":[{"name":"Institut Paoli-Calmettes, Service d\u2019H\u00e9matologie , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Collette","sequence":"additional","affiliation":[{"name":"INSERM, Unit\u00e9 891, Centre de Recherche en Canc\u00e9rologie de Marseille, Institut Paoli-Calmettes, TrGET Plateforme d'Essais Pr\u00e9cliniques , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Vey","sequence":"additional","affiliation":[{"name":"Institut Paoli-Calmettes, Service d\u2019H\u00e9matologie , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Olive","sequence":"additional","affiliation":[{"name":"Institut Paoli-Calmettes, Laboratoire d\u2019Immunologie des Tumeurs , Marseille F-13009 ,","place":["France"]},{"name":"INSERM, Unit\u00e9 891, Centre de Recherche en Canc\u00e9rologie de Marseille, Immunit\u00e9 et Cancers , Marseille F-13009 ,","place":["France"]},{"name":"Institut Paoli-Calmettes, Plateforme d'Immunomonitoring en Canc\u00e9rologie , Marseille F-13009 ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,5,1]]},"reference":[{"key":"2025032410051349100_r1","first-page":"233","article-title":"Graft-versus-leukemia following bone marrow transplantation","volume":"2","author":"Butturini","year":"1987","journal-title":"Bone Marrow Transplant."},{"key":"2025032410051349100_r2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1182\/blood.V75.3.555.555","article-title":"Graft-versus-leukemia reactions after bone marrow transplantation","volume":"75","author":"Horowitz","year":"1990","journal-title":"Blood"},{"key":"2025032410051349100_r3","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1038\/sj.leu.2403930","article-title":"Antigen-specific cellular immunotherapy of leukemia","volume":"19","author":"Van Driessche","year":"2005","journal-title":"Leukemia"},{"key":"2025032410051349100_r4","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1056\/NEJMoa0811036","article-title":"Loss of mismatched HLA in leukemia after stem-cell transplantation","volume":"361","author":"Vago","year":"2009","journal-title":"N. 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