{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:21:37Z","timestamp":1791112897599,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>IL-7 is a cytokine crucial for development and maintenance of lymphocytes and other hematopoietic cells. However, how IL-7\u2013expressing cells are distributed in lymphoid organs is not well known. To address this question, we established and analyzed IL-7\u2013GFP knock-in mice. Thymic epithelial cells (TECs) expressed high GFP levels in the cortex and medulla, as detected with an anti-GFP Ab. Thymic mesenchymal cells also expressed GFP. Flow cytometry analysis suggested that cortical TECs expressed higher GFP levels than did medullary TECs. In bone marrow, immunohistochemistry indicated high levels of GFP in many VCAM-1+ mesenchymal stromal cells and in some VCAM-1\u2212 cells. Additionally, half of the VCAM-1+CD31\u2212 stromal cells and some platelet-derived growth factor receptor \u03b1+ stromal cells were GFP+, as detected by flow cytometry. Moreover, we detected GFP expression in fibroblastic reticular cells in the T cell zone and cortical ridge of lymph nodes. Remarkably, lymphatic endothelial cells (LECs) expressed GFP at high levels within the lymph node medulla, skin epidermis, and intestinal tissues. Additionally, we detected abundant IL-7 transcripts in isolated LECs, suggesting that LECs produce IL-7, a heretofore unknown finding. Furthermore, GFP is expressed in a subpopulation of intestinal epithelial cells, and that expression was markedly upregulated in a dextran sulfate sodium-induced acute colitis model. Overall, IL-7\u2013GFP knock-in mice serve as a unique and powerful tool to examine the identity and distribution of IL-7\u2013expressing cells in vivo.<\/jats:p>","DOI":"10.4049\/jimmunol.1200586","type":"journal-article","created":{"date-parts":[[2012,7,12]],"date-time":"2012-07-12T01:37:39Z","timestamp":1342057059000},"page":"1577-1584","source":"Crossref","is-referenced-by-count":132,"title":["Identification of IL-7\u2013Producing Cells in Primary and Secondary Lymphoid Organs Using IL-7\u2013GFP Knock-In Mice"],"prefix":"10.1093","volume":"189","author":[{"given":"Takahiro","family":"Hara","sequence":"first","affiliation":[{"name":"Laboratory of Biological Protection, Department of Biological Responses, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichiro","family":"Shitara","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Protection, Department of Biological Responses, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]},{"name":"Graduate School of Biostudies, Kyoto University , Kyoto 606-8501,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumiko","family":"Imai","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Protection, Department of Biological Responses, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Miyachi","sequence":"additional","affiliation":[{"name":"Reproductive Engineering Team, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satsuki","family":"Kitano","sequence":"additional","affiliation":[{"name":"Reproductive Engineering Team, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisayuki","family":"Yao","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Protection, Department of Biological Responses, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]},{"name":"Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital , Kyoto 606-8507,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizue","family":"Tani-ichi","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Protection, Department of Biological Responses, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Ikuta","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Protection, Department of Biological Responses, Institute for Virus Research, Kyoto University , Kyoto 606-8507,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,8,15]]},"reference":[{"key":"2025032409220312300_r1","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1084\/jem.181.4.1519","article-title":"Lymphopenia in interleukin (IL)-7 gene-deleted mice identifies IL-7 as a nonredundant cytokine","volume":"181","author":"von Freeden-Jeffry","year":"1995","journal-title":"J. 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