{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:43:03Z","timestamp":1790322183458,"version":"4.1.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although Listeria monocytogenes can induce systemic infection causing spontaneous abortion, septicemia, and meningitis, studies have not been performed to investigate human anti-L. monocytogenes immune responses, including those of Ag-specific V\u03b32V\u03b42 T cells, a dominant human \u03b3\u03b4 T cell subset. L. monocytogenes is the only pathogen known to possess both the mevalonate and non-mevalonate isoprenoid biosynthesis pathways that produce metabolic phosphates or phosphoantigens activating human V\u03b32V\u03b42 T cells, making it interesting to explore in vivo anti-L. monocytogenes immune responses of V\u03b32V\u03b42 T cells. In this study, we demonstrated that subclinical systemic L. monocytogenes infection of rhesus macaques via parenteral inoculation or vaccination with an attenuated Listeria strain induced multieffector-functional immune responses of phosphoantigen-specific V\u03b32V\u03b42 T cells. Subclinical systemic infection and reinfection with attenuated L. monocytogenes uncovered the ability of V\u03b32V\u03b42 T cells to mount expansion and adaptive or recall-like expansion. Expanded V\u03b32V\u03b42 T cells could traffic to and accumulate in the pulmonary compartment and intestinal mucosa. Expanded V\u03b32V\u03b42 T cells could evolve into effector cells producing IFN-\u03b3, TNF-\u03b1, IL-4, IL-17, or perforin after L. monocytogenes infection, and some effector V\u03b32V\u03b42 T cells could coproduce IL-17 and IFN-\u03b3, IL-4 and IFN-\u03b3, or TNF-\u03b1 and perforin. Surprisingly, in vivo-expanded V\u03b32V\u03b42 T effector cells in subclinical L. monocytogenes infection could directly lyse L. monocytogenes-infected target cells and inhibit intracellular L. monocytogenes bacteria. Thus, we present the first demonstration, to our knowledge, of multieffector-functional V\u03b32V\u03b42 T cell responses against L. monocytogenes.<\/jats:p>","DOI":"10.4049\/jimmunol.1200641","type":"journal-article","created":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T04:56:25Z","timestamp":1340859385000},"page":"1285-1293","source":"Crossref","is-referenced-by-count":64,"title":["Multieffector-Functional Immune Responses of HMBPP-Specific V\u03b32V\u03b42 T Cells in Nonhuman Primates Inoculated with <i>Listeria monocytogenes \u0394actA prfA<\/i>\n                  <b>*<\/b>"],"prefix":"10.1093","volume":"189","author":[{"given":"Bridgett","family":"Ryan-Payseur","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine , Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Frencher","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine , Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Shen","sequence":"additional","affiliation":[{"name":"Harvard Medical School, Beth Israel Deaconess Medical Center , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crystal Y","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine , Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine , Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng W","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine , Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,8,1]]},"reference":[{"key":"2025032409215989700_r1","doi-asserted-by":"crossref","first-page":"8175","DOI":"10.1073\/pnas.91.17.8175","article-title":"Nonpeptide ligands for human gamma delta T cells","volume":"91","author":"Tanaka","year":"1994","journal-title":"Proc. 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