{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:35:14Z","timestamp":1791398114274,"version":"4.3.3"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>CD4+ Th2 cytokine responses promote the development of allergic inflammation and are critical for immunity to parasitic helminth infection. Recent studies highlighted that basophils can promote Th2 cytokine-mediated inflammation and that phenotypic and functional heterogeneity exists between classical IL-3\u2013elicited basophils and thymic stromal lymphopoietin (TSLP)-elicited basophils. However, whether distinct basophil populations develop after helminth infection and their relative contributions to anti-helminth immune responses remain to be defined. After Trichinella spiralis infection of mice, we show that basophil responses are rapidly induced in multiple tissue compartments, including intestinal-draining lymph nodes. Trichinella-induced basophil responses were IL-3\u2013IL-3R independent but critically dependent on TSLP\u2013TSLPR interactions. Selective depletion of basophils after Trichinella infection impaired infection-induced CD4+ Th2 cytokine responses, suggesting that TSLP-dependent basophils augment Th2 cytokine responses after helminth infection. The identification and functional classification of TSLP-dependent basophils in a helminth infection model, coupled with their recently described role in promoting atopic dermatitis, suggests that these cells may be a critical population in promoting Th2 cytokine-associated inflammation in a variety of inflammatory or infectious settings. Collectively, these data suggest that the TSLP\u2013basophil pathway may represent a new target in the design of therapeutic intervention strategies to promote or limit Th2 cytokine-dependent immunity and inflammation.<\/jats:p>","DOI":"10.4049\/jimmunol.1200691","type":"journal-article","created":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T02:09:42Z","timestamp":1349057382000},"page":"4371-4378","source":"Crossref","is-referenced-by-count":94,"title":["Thymic Stromal Lymphopoietin-Dependent Basophils Promote Th2 Cytokine Responses following Intestinal Helminth Infection"],"prefix":"10.1093","volume":"189","author":[{"given":"Paul R","family":"Giacomin","sequence":"first","affiliation":[{"name":"Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104"},{"name":"Queensland Tropical Health Alliance and School of Public Health and Tropical Medicine, James Cook University , Cairns, Queensland 4878,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark C","family":"Siracusa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P","family":"Walsh","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard K","family":"Grencis","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, University of Manchester , Manchester M13 9PT,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Kubo","sequence":"additional","affiliation":[{"name":"Laboratory for Signal Network, Research Center for Allergy and Immunology, RIKEN Yokohama Institute , Yokohama 230-0045,","place":["Japan"]},{"name":"Division of Molecular Pathology, Research Institute for Biological Science, Tokyo University of Science , Chiba 278-0022,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R","family":"Comeau","sequence":"additional","affiliation":[{"name":"Inflammation Research, Amgen Inc. , Seattle, WA 98119"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Artis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19104"},{"name":"Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,11,1]]},"reference":[{"key":"2025032409332494500_r1","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1038\/nri2199","article-title":"Protective immune mechanisms in helminth infection","volume":"7","author":"Anthony","year":"2007","journal-title":"Nat. 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Immunol."},{"key":"2025032409332494500_r40","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1084\/jem.20051135","article-title":"TSLP-activated dendritic cells induce an inflammatory T helper type 2 cell response through OX40 ligand","volume":"202","author":"Ito","year":"2005","journal-title":"J. Exp. Med."},{"key":"2025032409332494500_r41","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.4049\/jimmunol.145.8.2500","article-title":"IL-3 promotes production of IL-4 by splenic non-B, non-T cells in response to Fc receptor cross-linkage","volume":"145","author":"Le Gros","year":"1990","journal-title":"J. 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