{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:30:20Z","timestamp":1761489020784,"version":"3.40.3"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Viral infection is a major cause of morbidity and mortality, and there are few therapeutic options available to augment a virus-specific T cell response. Although allo-HLA cross-reactivity from virus-specific memory T cells is common, it is unclear whether priming with specific allogeneic cells could conversely elicit a viral peptide\/self-HLA restricted cytotoxic T cell response in humans. First, we used the previously described allo-HLA-B*44:02 cross-reactivity of EBV peptide\/HLA-B8 restricted T cells, to determine whether allogeneic HLA stimulation can elicit a cytolytic immune response against EBV. HLA-B8+ HLA-B44\u2212 EBV-seropositive PBMCs were stimulated with either HLA-B*44:02+ or HLA-B*44:03+ mismatched irradiated PBMCs in a 7\u201310 d MLR. The allo-HLA stimulated responder cells were then evaluated for cytotoxicity using EBV peptide loaded autologous target cells and unloaded HLA-B8+ EBV LCL target cells. PBMCs from EBV-seropositive donors gained EBV-specific cytolytic effector function following specific allo-HLA stimulation. Finally, we also elicited cytolytic CMV-specific responses using specific allogeneic cell stimulation, to confirm that this technique can be used to elicit viral peptide\/self-HLA restricted responses even from nonpublic TCR responses. Allogeneic cell stimulation used as a cell therapy may be a potential tool to augment an antiviral T cell response in patients with EBV or CMV infection.<\/jats:p>","DOI":"10.4049\/jimmunol.1201034","type":"journal-article","created":{"date-parts":[[2012,10,18]],"date-time":"2012-10-18T12:46:10Z","timestamp":1350564370000},"page":"4825-4831","source":"Crossref","is-referenced-by-count":12,"title":["Stimulation of Human EBV- and CMV-Specific Cytolytic Effector Function Using Allogeneic HLA Molecules"],"prefix":"10.1093","volume":"189","author":[{"given":"Lloyd J","family":"D\u2019Orsogna","sequence":"first","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre , Leiden 2300 RC,","place":["The Netherlands"]},{"name":"School of Pathology and Laboratory Medicine, University of Western Australia , Perth 6009, Western Australia,","place":["Australia"]},{"name":"Department of Clinical Immunology and PathWest Laboratory Medicine, Royal Perth Hospital , Perth 6000, Western Australia,","place":["Australia"]},{"name":"Institute for Immunology and Infectious Diseases, Murdoch University , Perth 6150, Western Australia,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heleen","family":"van den Heuvel","sequence":"additional","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre , Leiden 2300 RC,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen M W","family":"van der Meer-Prins","sequence":"additional","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre , Leiden 2300 RC,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dave L","family":"Roelen","sequence":"additional","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre , Leiden 2300 RC,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilias I N","family":"Doxiadis","sequence":"additional","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre , Leiden 2300 RC,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frans H J","family":"Claas","sequence":"additional","affiliation":[{"name":"Department of Immunohematology and Blood Transfusion, Leiden University Medical Centre , Leiden 2300 RC,","place":["The Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,11,15]]},"reference":[{"key":"2025032409350396400_r1","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1084\/jem.186.9.1407","article-title":"Phenotypic and functional separation of memory and effector human CD8+ T cells","volume":"186","author":"Hamann","year":"1997","journal-title":"J. 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