{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T16:31:29Z","timestamp":1764001889279,"version":"3.40.3"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Sialoadhesin (Sn) is a sialic acid\u2013binding Ig-like lectin expressed selectively on macrophage subsets. In a model of experimental autoimmune encephalomyelitis, Sn interacted with sialylated ligands expressed selectively on CD4+Foxp3+ regulatory T cells (Tregs) and inhibited their proliferation. In this study, we examined the induction of Sn ligands (SnL) on all splenic CD4+ T cells following in vitro activation. Most CD4+ Tregs strongly upregulated SnL, whereas only a small subset of \u223c20% CD4+Foxp3\u2212 T cells (effector T cells [Teffs]) upregulated SnL. SnL+ Teffs displayed higher levels of activation markers CD25 and CD69, exhibited increased proliferation, and produced higher amounts of IL-2 and IFN-\u03b3 than corresponding SnL\u2212 Teffs. Coculture of activated Teffs with Sn+ macrophages or Sn+ Chinese hamster ovary cells resulted in increased cell death, suggesting a regulatory role for Sn\u2013SnL interactions. The key importance of \u03b12,3-sialylation in SnL expression was demonstrated by increased binding of \u03b12,3-linkage\u2013specific Maackia amurensis lectin, increased expression of \u03b12,3-sialyltransferase ST3GalVI, and loss of SnL following treatment with an \u03b12,3-linkage\u2013specific sialidase. The induction of SnL on activated CD4+ T cells was dependent on N-glycan rather than O-glycan biosynthesis and independent of the mucin-like molecules CD43 and P-selectin glycoprotein ligand-1, previously implicated in Sn interactions. Induction of ligands on CD4+Foxp3\u2212 Teffs was also observed in vivo using the New Zealand Black \u00d7 New Zealand White F1 murine model of spontaneous lupus and SnL levels on Teffs correlated strongly with the degree of proteinuria. Collectively, these data indicate that SnL is a novel marker of activated CD4+ Teffs that are implicated in the pathogenesis of autoimmune diseases.<\/jats:p>","DOI":"10.4049\/jimmunol.1201172","type":"journal-article","created":{"date-parts":[[2013,2,14]],"date-time":"2013-02-14T04:36:51Z","timestamp":1360816611000},"page":"2593-2602","source":"Crossref","is-referenced-by-count":25,"title":["Sialoadhesin Ligand Expression Identifies a Subset of CD4+Foxp3\u2212 T Cells with a Distinct Activation and Glycosylation Profile"],"prefix":"10.1093","volume":"190","author":[{"given":"Dana","family":"Kidder","sequence":"first","affiliation":[{"name":"Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee , Dundee DD1 5EH,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah E","family":"Richards","sequence":"additional","affiliation":[{"name":"Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee , Dundee DD1 5EH,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann J","family":"Ziltener","sequence":"additional","affiliation":[{"name":"The Biomedical Research Centre , Vancouver, British Columbia V6T 1Z3,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver A","family":"Garden","sequence":"additional","affiliation":[{"name":"Regulatory T Cell Laboratory, The Royal Veterinary College , London NW1 0TU,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R","family":"Crocker","sequence":"additional","affiliation":[{"name":"Division of Cell Signalling and Immunology, College of Life Sciences, University of Dundee , Dundee DD1 5EH,","place":["United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,3,15]]},"reference":[{"key":"2025032406422430200_r1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nri2056","article-title":"Siglecs and their roles in the immune system","volume":"7","author":"Crocker","year":"2007","journal-title":"Nat. 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