{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:10:38Z","timestamp":1790363438340,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Gram-positive bacteria are an important public health problem, but it is unclear how they cause systemic inflammation in sepsis. Our previous work showed that peptidoglycan (PGN) induced proinflammatory cytokines in human cells by binding to an unknown extracellular receptor, followed by phagocytosis leading to the generation of NOD ligands. In this study, we used flow cytometry to identify host factors that supported PGN binding to immune cells. PGN binding required plasma, and plasma from all tested healthy donors contained IgG recognizing PGN. Plasma depleted of IgG or of anti-PGN Abs did not support PGN binding or PGN-triggered cytokine production. Adding back intact but not F(ab\u2032)2 IgG restored binding and cytokine production. Transfection of HEK293 cells with Fc\u03b3RIIA enabled PGN binding and phagocytosis. These data establish a key role for anti-PGN IgG and Fc\u03b3Rs in supporting inflammation to a major structural element of Gram-positive bacteria and suggest that anti-PGN IgG contributes to human pathology in Gram-positive sepsis.<\/jats:p>","DOI":"10.4049\/jimmunol.1201302","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T12:00:22Z","timestamp":1342699222000},"page":"2423-2431","source":"Crossref","is-referenced-by-count":33,"title":["Anti-Peptidoglycan Antibodies and Fc\u03b3 Receptors Are the Key Mediators of Inflammation in Gram-Positive Sepsis"],"prefix":"10.1093","volume":"189","author":[{"given":"Dawei","family":"Sun","sequence":"first","affiliation":[{"name":"Immunobiology and Cancer Program, Oklahoma Medical Research Foundation , Oklahoma City, OK 73104"},{"name":"Department of Cell Biology, University of Oklahoma , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent","family":"Raisley","sequence":"additional","affiliation":[{"name":"Immunobiology and Cancer Program, Oklahoma Medical Research Foundation , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marybeth","family":"Langer","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Division, Department of Medicine, Oklahoma University Health Sciences Center , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janaki K","family":"Iyer","sequence":"additional","affiliation":[{"name":"Immunobiology and Cancer Program, Oklahoma Medical Research Foundation , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidya","family":"Vedham","sequence":"additional","affiliation":[{"name":"Methods and Technologies Branch, Epidemiology and Genomics Research Program, Division of Cancer Control and Population Sciences, National Cancer Institute , Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy L","family":"Ballard","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Oklahoma , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith A","family":"James","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Metcalf","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Division, Department of Medicine, Oklahoma University Health Sciences Center , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K Mark","family":"Coggeshall","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Oklahoma , Oklahoma City, OK 73104"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,9,1]]},"reference":[{"key":"2025032409231645200_r1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1086\/421946","article-title":"Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study","volume":"39","author":"Wisplinghoff","year":"2004","journal-title":"Clin. 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