{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T20:31:08Z","timestamp":1791923468249,"version":"4.3.4"},"reference-count":92,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The role of B cells in host defense against fungi has been difficult to establish. We quantified and determined the molecular derivation of B-1a, B-1b, and B-2 B cell populations in C57BL\/6 mice after pulmonary infection with Cryptococcus neoformans. Total B-1 and B-2 cell numbers increased in lungs and peritoneal cavity as early as day 1 postinfection, but lacked signs of clonal expansion. Labeled capsular (24067) and acapsular (Cap67) C. neoformans strains were used to identify C. neoformans\u2013binding B cell subsets by flow cytometry. Peritoneal cavity B-1a B cells exhibited the most acapsular and capsular C. neoformans binding in C. neoformans\u2013infected mice, and C. neoformans\u2013selected B-1 B cells secreted laminarin- and C. neoformans\u2013binding IgM. Single-cell PCR-based sequence analysis of B-1a, B-1b, and B-2 cell IgH V region H chain (VH) genes revealed increased usage of VH11 and VH12, respectively, in acapsular and capsular C. neoformans\u2013selected B-1a cells. Germline VH segments were used, with capsular C. neoformans\u2013selected cells having less junctional diversity than acapsular C. neoformans\u2013selected cells. Further studies in B-1 B cell\u2013depleted mice showed that these mice had higher brain and lung fungal burdens and less alveolar macrophage phagocytosis of C. neoformans than did control and B-1a B cell\u2013reconstituted mice. Taken together, these results establish a mechanistic role for B-1 B cells in the innate B cell response to pulmonary infection with C. neoformans and reveal that IgM-producing B-1a cells, which express germline VH genes, bind C. neoformans and contribute to early fungal clearance. Thus, B-1a B cells provide a first line of defense during pulmonary C. neoformans infection in mice.<\/jats:p>","DOI":"10.4049\/jimmunol.1201514","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T02:10:13Z","timestamp":1353636613000},"page":"5820-5830","source":"Crossref","is-referenced-by-count":46,"title":["Molecular Characterization of the Early B Cell Response to Pulmonary <i>Cryptococcus neoformans<\/i> Infection"],"prefix":"10.1093","volume":"189","author":[{"given":"Soma","family":"Rohatgi","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center , Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liise-anne","family":"Pirofski","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine and Montefiore Medical Center , Bronx, NY 10461"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine , Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,12,15]]},"reference":[{"key":"2025032409400891100_r1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/jlb.55.1.35","article-title":"The role of CD4+ and CD8+ T cells in the protective inflammatory response to a pulmonary cryptococcal infection","volume":"55","author":"Huffnagle","year":"1994","journal-title":"J. Leukoc. Biol."},{"key":"2025032409400891100_r2","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1084\/jem.173.4.793","article-title":"Immunity to a pulmonary Cryptococcus neoformans infection requires both CD4+ and CD8+ T cells","volume":"173","author":"Huffnagle","year":"1991","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r3","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1128\/iai.55.3.742-748.1987","article-title":"Production, characterization, and antibody specificity of a mouse monoclonal antibody reactive with Cryptococcus neoformans capsular polysaccharide","volume":"55","author":"Dromer","year":"1987","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r4","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/iai.55.8.1895-1899.1987","article-title":"Production and characterization of monoclonal antibodies specific for Cryptococcus neoformans capsular polysaccharide","volume":"55","author":"Eckert","year":"1987","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r5","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1128\/iai.63.2.573-579.1995","article-title":"Antibodies to Cryptococcus neoformans glucuronoxylomannan enhance antifungal activity of murine macrophages","volume":"63","author":"Mukherjee","year":"1995","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r6","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1128\/AAC.42.6.1437","article-title":"Characterization of a murine monoclonal antibody to Cryptococcus neoformans polysaccharide that is a candidate for human therapeutic studies","volume":"42","author":"Casadevall","year":"1998","journal-title":"Antimicrob. Agents Chemother."},{"key":"2025032409400891100_r7","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.4049\/jimmunol.166.2.1087","article-title":"A cryptococcal capsular polysaccharide mimotope prolongs the survival of mice with Cryptococcus neoformans infection","volume":"166","author":"Fleuridor","year":"2001","journal-title":"J. Immunol."},{"key":"2025032409400891100_r8","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1128\/iai.55.3.749-752.1987","article-title":"Protection of mice against experimental cryptococcosis by anti-Cryptococcus neoformans monoclonal antibody","volume":"55","author":"Dromer","year":"1987","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r9","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.1128\/iai.58.6.1914-1918.1990","article-title":"Opsonization of Cryptococcus neoformans by a family of isotype-switch variant antibodies specific for the capsular polysaccharide","volume":"58","author":"Schlageter","year":"1990","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r10","doi-asserted-by":"crossref","first-page":"2598","DOI":"10.1128\/IAI.70.5.2598-2604.2002","article-title":"Immunoglobulin G monoclonal antibodies to Cryptococcus neoformans protect mice deficient in complement component C3","volume":"70","author":"Shapiro","year":"2002","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r11","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1128\/iai.58.3.639-645.1990","article-title":"Human natural killer cells do not inhibit growth of Cryptococcus neoformans in the absence of antibody","volume":"58","author":"Miller","year":"1990","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r12","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1128\/iai.51.2.556-562.1986","article-title":"Antibody-dependent natural killer cell-mediated growth inhibition of Cryptococcus neoformans","volume":"51","author":"Nabavi","year":"1986","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r13","doi-asserted-by":"crossref","first-page":"e5392","DOI":"10.1371\/journal.pone.0005392","article-title":"Protection by anti-\u03b2-glucan antibodies is associated with restricted \u03b2-1,3 glucan binding specificity and inhibition of fungal growth and adherence","volume":"4","author":"Torosantucci","year":"2009","journal-title":"PLoS ONE"},{"key":"2025032409400891100_r14","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1084\/jem.20050749","article-title":"A novel glyco-conjugate vaccine against fungal pathogens","volume":"202","author":"Torosantucci","year":"2005","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r15","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1084\/jem.177.4.1105","article-title":"Molecular characterization of the humoral responses to Cryptococcus neoformans infection and glucuronoxylomannan-tetanus toxoid conjugate immunization","volume":"177","author":"Mukherjee","year":"1993","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r16","doi-asserted-by":"crossref","first-page":"3864","DOI":"10.1128\/iai.62.9.3864-3872.1994","article-title":"Molecular and idiotypic analysis of antibodies to Cryptococcus neoformans glucuronoxylomannan","volume":"62","author":"Casadevall","year":"1994","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r17","doi-asserted-by":"crossref","first-page":"4810","DOI":"10.1128\/IAI.72.8.4810-4818.2004","article-title":"Protective and nonprotective human immunoglobulin M monoclonal antibodies to Cryptococcus neoformans glucuronoxylomannan manifest different specificities and gene use profiles","volume":"72","author":"Maitta","year":"2004","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r18","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/IAI.72.1.196-208.2004","article-title":"Immunogenicity and efficacy of Cryptococcus neoformans capsular polysaccharide glucuronoxylomannan peptide mimotope-protein conjugates in human immunoglobulin transgenic mice","volume":"72","author":"Maitta","year":"2004","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r19","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/S0966-842X(01)02134-5","article-title":"Polysaccharides, mimotopes and vaccines for fungal and encapsulated pathogens","volume":"9","author":"Pirofski","year":"2001","journal-title":"Trends Microbiol."},{"key":"2025032409400891100_r20","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/nri2524","article-title":"B cells in HIV infection and disease","volume":"9","author":"Moir","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"2025032409400891100_r21","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.jaci.2004.10.048","article-title":"The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency","volume":"115","author":"Carsetti","year":"2005","journal-title":"J. Allergy Clin. Immunol."},{"key":"2025032409400891100_r22","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1084\/jem.20100034","article-title":"Conserved natural IgM antibodies mediate innate and adaptive immunity against the opportunistic fungus Pneumocystis murina","volume":"207","author":"Rapaka","year":"2010","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r23","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nri2901","article-title":"The double life of a B-1 cell: self-reactivity selects for protective effector functions","volume":"11","author":"Baumgarth","year":"2011","journal-title":"Nat. Rev. Immunol."},{"key":"2025032409400891100_r24","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1086\/599318","article-title":"IgM+ memory B cell expression predicts HIV-associated cryptococcosis status","volume":"200","author":"Subramaniam","year":"2009","journal-title":"J. Infect. Dis."},{"key":"2025032409400891100_r25","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1086\/315102","article-title":"Quantitative and qualitative differences in the serum antibody profiles of human immunodeficiency virus-infected persons with and without Cryptococcus neoformans meningitis","volume":"180","author":"Fleuridor","year":"1999","journal-title":"J. Infect. Dis."},{"key":"2025032409400891100_r26","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.1128\/iai.63.8.3005-3014.1995","article-title":"Analysis of human monoclonal antibodies elicited by vaccination with a Cryptococcus neoformans glucuronoxylomannan capsular polysaccharide vaccine","volume":"63","author":"Pirofski","year":"1995","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r27","doi-asserted-by":"crossref","first-page":"5755","DOI":"10.4049\/jimmunol.0901638","article-title":"The absence of serum IgM enhances the susceptibility of mice to pulmonary challenge with Cryptococcus neoformans","volume":"184","author":"Subramaniam","year":"2010","journal-title":"J. Immunol."},{"key":"2025032409400891100_r28","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1111\/j.1462-5822.2006.00786.x","article-title":"Host defence against C. albicans infections in IgH transgenic mice with VH derived from a natural anti-keratin antibody","volume":"9","author":"Li","year":"2007","journal-title":"Cell. Microbiol."},{"key":"2025032409400891100_r29","doi-asserted-by":"crossref","first-page":"5085","DOI":"10.1128\/IAI.00278-07","article-title":"An anti-\u03b2-glucan monoclonal antibody inhibits growth and capsule formation of Cryptococcus neoformans in vitro and exerts therapeutic, anticryptococcal activity in vivo","volume":"75","author":"Rachini","year":"2007","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/iai.26.1.1-3.1979","article-title":"Experimental cryptococcosis in normal and B-cell-deficient mice","volume":"26","author":"Monga","year":"1979","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r31","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1128\/iai.65.2.525-530.1997","article-title":"A role for B cells in resistance to Cryptococcus neoformans in mice","volume":"65","author":"Aguirre","year":"1997","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r32","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1128\/IAI.70.3.1571-1580.2002","article-title":"Antibody-mediated protection in murine Cryptococcus neoformans infection is associated with pleotrophic effects on cytokine and leukocyte responses","volume":"70","author":"Feldmesser","year":"2002","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r33","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1128\/IAI.73.2.1141-1150.2005","article-title":"Antibody-mediated protection against Cryptococcus neoformans pulmonary infection is dependent on B cells","volume":"73","author":"Rivera","year":"2005","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r34","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1128\/iai.47.1.282-287.1985","article-title":"Genetic resistance to murine cryptococcosis: increased susceptibility in the CBA\/N XID mutant strain of mice","volume":"47","author":"Marquis","year":"1985","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r35","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.immuni.2005.04.011","article-title":"B-1a and B-1b cells exhibit distinct developmental requirements and have unique functional roles in innate and adaptive immunity to S. pneumoniae","volume":"23","author":"Haas","year":"2005","journal-title":"Immunity"},{"key":"2025032409400891100_r36","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.4049\/jimmunol.177.5.2749","article-title":"B-1 B cell development","volume":"177","author":"Hardy","year":"2006","journal-title":"J. Immunol."},{"key":"2025032409400891100_r37","doi-asserted-by":"crossref","first-page":"5394","DOI":"10.1073\/pnas.1121632109","article-title":"Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells","volume":"109","author":"Ghosn","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032409400891100_r38","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1111\/j.0105-2896.2004.0109.x","article-title":"Peripheral development of B cells in mouse and man","volume":"197","author":"Carsetti","year":"2004","journal-title":"Immunol. Rev."},{"key":"2025032409400891100_r39","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1093\/intimm\/13.9.1193","article-title":"Mouse B-1 cell-derived mononuclear phagocyte, a novel cellular component of acute non-specific inflammatory exudate","volume":"13","author":"Almeida","year":"2001","journal-title":"Int. Immunol."},{"key":"2025032409400891100_r40","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1189\/jlb.1005603","article-title":"Nitric oxide-dependent killing of Cryptococcus neoformans by B-1-derived mononuclear phagocyte","volume":"80","author":"Ghosn","year":"2006","journal-title":"J. Leukoc. Biol."},{"key":"2025032409400891100_r41","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.4049\/jimmunol.1200138","article-title":"\u03bc-Chain-deficient mice possess B-1 cells and produce IgG and IgE, but Not IgA, following systemic sensitization and inhalational challenge in a fungal asthma model","volume":"189","author":"Ghosh","year":"2012","journal-title":"J. Immunol."},{"key":"2025032409400891100_r42","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1128\/IAI.00506-09","article-title":"Improved survival of mice deficient in secretory immunoglobulin M following systemic infection with Cryptococcus neoformans","volume":"78","author":"Subramaniam","year":"2010","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r43","doi-asserted-by":"crossref","first-page":"4534","DOI":"10.1128\/iai.60.11.4534-4541.1992","article-title":"Protective murine monoclonal antibodies to Cryptococcus neoformans","volume":"60","author":"Mukherjee","year":"1992","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r44","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1016\/j.ajpath.2011.12.038","article-title":"IL-23 dampens the allergic response to Cryptococcus neoformans through IL-17-independent and -dependent mechanisms","volume":"180","author":"Szymczak","year":"2012","journal-title":"Am. J. Pathol."},{"key":"2025032409400891100_r45","doi-asserted-by":"crossref","first-page":"4343","DOI":"10.1073\/pnas.0813411106","article-title":"Antigen-specific B-1a antibodies induced by Francisella tularensis LPS provide long-term protection against F. tularensis LVS challenge","volume":"106","author":"Cole","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032409400891100_r46","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0022-1759(02)00499-4","article-title":"Identification of an antigen-specific B cell population","volume":"272","author":"Newman","year":"2003","journal-title":"J. Immunol. Methods"},{"key":"2025032409400891100_r47","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1128\/EC.00122-07","article-title":"Self-aggregation of Cryptococcus neoformans capsular glucuronoxylomannan is dependent on divalent cations","volume":"6","author":"Nimrichter","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"2025032409400891100_r48","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.jim.2009.08.009","article-title":"Cloning and expression of murine Ig genes from single B cells","volume":"350","author":"Tiller","year":"2009","journal-title":"J. Immunol. Methods"},{"key":"2025032409400891100_r49","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1111\/j.1749-6632.1995.tb55831.x","article-title":"Development of the antibody repertoire as revealed by single-cell PCR of FACS-sorted B-cell subsets","volume":"764","author":"Kantor","year":"1995","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2025032409400891100_r50","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.jim.2008.09.017","article-title":"Systematic design and testing of nested (RT-)PCR primers for specific amplification of mouse rearranged\/expressed immunoglobulin variable region genes from small number of B cells","volume":"339","author":"Rohatgi","year":"2008","journal-title":"J. Immunol. Methods"},{"key":"2025032409400891100_r51","doi-asserted-by":"crossref","first-page":"5570","DOI":"10.4049\/jimmunol.0803254","article-title":"Molecular dissection of antibody responses against pneumococcal surface protein A: evidence for diverse DH-less heavy chain gene usage and avidity maturation","volume":"182","author":"Rohatgi","year":"2009","journal-title":"J. Immunol."},{"key":"2025032409400891100_r52","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/j.0959-9673.2005.00433.x","article-title":"B-1 cells are pivotal for in vivo inflammatory giant cell formation","volume":"86","author":"Bogsan","year":"2005","journal-title":"Int. J. Exp. Pathol."},{"key":"2025032409400891100_r53","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1093\/intimm\/7.5.877","article-title":"Prevention of autoimmune symptoms in autoimmune-prone mice by elimination of B-1 cells","volume":"7","author":"Murakami","year":"1995","journal-title":"Int. Immunol."},{"key":"2025032409400891100_r54","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1080\/08916930801890280","article-title":"Role of CD5+ B-1 cells in EAE pathogenesis","volume":"41","author":"Peterson","year":"2008","journal-title":"Autoimmunity"},{"key":"2025032409400891100_r55","doi-asserted-by":"crossref","first-page":"4393","DOI":"10.4049\/jimmunol.0903239","article-title":"B cell subsets contribute to renal injury and renal protection after ischemia\/reperfusion","volume":"185","author":"Renner","year":"2010","journal-title":"J. Immunol."},{"key":"2025032409400891100_r56","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.4049\/jimmunol.158.3.1175","article-title":"An unbiased analysis of VH-D-JH sequences from B-1a, B-1b, and conventional B cells","volume":"158","author":"Kantor","year":"1997","journal-title":"J. Immunol."},{"key":"2025032409400891100_r57","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1002\/j.1460-2075.1995.tb07157.x","article-title":"B-1a, B-1b and B-2 B cells display unique VHDJH repertoires formed at different stages of ontogeny and under different selection pressures","volume":"14","author":"Tornberg","year":"1995","journal-title":"EMBO J."},{"key":"2025032409400891100_r58","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1002\/j.1460-2075.1992.tb05092.x","article-title":"Immunoglobulin VH clan and family identity predicts variable domain structure and may influence antigen binding","volume":"11","author":"Kirkham","year":"1992","journal-title":"EMBO J."},{"key":"2025032409400891100_r59","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0161-5890(03)00149-4","article-title":"The function of conserved amino acids in or near the complementarity determining regions for related antibodies to Cryptococcus neoformans glucuronoxylomannan","volume":"40","author":"Nakouzi","year":"2003","journal-title":"Mol. Immunol."},{"key":"2025032409400891100_r60","doi-asserted-by":"crossref","first-page":"6060","DOI":"10.4049\/jimmunol.162.10.6060","article-title":"IgM heavy chain complementarity-determining region 3 diversity is constrained by genetic and somatic mechanisms until two months after birth","volume":"162","author":"Shiokawa","year":"1999","journal-title":"J. Immunol."},{"key":"2025032409400891100_r61","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.1128\/IAI.00094-07","article-title":"The relative susceptibility of mouse strains to pulmonary Cryptococcus neoformans infection is associated with pleiotropic differences in the immune response","volume":"75","author":"Zaragoza","year":"2007","journal-title":"Infect. Immun."},{"key":"2025032409400891100_r62","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1084\/jem.20080979","article-title":"Dual role for B-1a cells in immunity to influenza virus infection","volume":"205","author":"Choi","year":"2008","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r63","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1002\/eji.200939798","article-title":"B-cell fate decisions following influenza virus infection","volume":"40","author":"Rothaeusler","year":"2010","journal-title":"Eur. J. Immunol."},{"key":"2025032409400891100_r64","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1084\/jem.192.2.271","article-title":"B-1 and B-2 cell-derived immunoglobulin M antibodies are nonredundant components of the protective response to influenza virus infection","volume":"192","author":"Baumgarth","year":"2000","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r65","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S1074-7613(01)00257-6","article-title":"CXCL13 is required for B1 cell homing, natural antibody production, and body cavity immunity","volume":"16","author":"Ansel","year":"2002","journal-title":"Immunity"},{"key":"2025032409400891100_r66","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.imbio.2009.03.009","article-title":"B-1 cells modulate the kinetics of wound-healing process in mice","volume":"215","author":"Oliveira","year":"2010","journal-title":"Immunobiology"},{"key":"2025032409400891100_r67","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1189\/jlb.0711372","article-title":"Pivotal advance: peritoneal cavity B-1 B cells have phagocytic and microbicidal capacities and present phagocytosed antigen to CD4+ T cells","volume":"91","author":"Parra","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"2025032409400891100_r68","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/j.imbio.2008.12.007","article-title":"Microbicidal property of B1 cell derived mononuclear phagocyte","volume":"214","author":"Popi","year":"2009","journal-title":"Immunobiology"},{"key":"2025032409400891100_r69","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1016\/j.vaccine.2009.12.021","article-title":"A \u03b2-glucan-conjugate vaccine and anti-\u03b2-glucan antibodies are effective against murine vaginal candidiasis as assessed by a novel in vivo imaging technique","volume":"28","author":"Pietrella","year":"2010","journal-title":"Vaccine"},{"key":"2025032409400891100_r70","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.4049\/jimmunol.170.7.3819","article-title":"The resolution of relapsing fever borreliosis requires IgM and is concurrent with expansion of B1b lymphocytes","volume":"170","author":"Alugupalli","year":"2003","journal-title":"J. Immunol."},{"key":"2025032409400891100_r71","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.immuni.2004.06.019","article-title":"B1b lymphocytes confer T cell-independent long-lasting immunity","volume":"21","author":"Alugupalli","year":"2004","journal-title":"Immunity"},{"key":"2025032409400891100_r72","doi-asserted-by":"crossref","first-page":"569","DOI":"10.4049\/jimmunol.1101446","article-title":"The nature of an in vivo anti-capsular polysaccharide response is markedly influenced by the composition and\/or architecture of the bacterial subcapsular domain","volume":"188","author":"Arjunaraja","year":"2012","journal-title":"J. Immunol."},{"key":"2025032409400891100_r73","doi-asserted-by":"crossref","first-page":"575","DOI":"10.4049\/jimmunol.1200599","article-title":"Differential idiotype utilization for the in vivo type 14 capsular polysaccharide-specific Ig responses to intact Streptococcus pneumoniae versus a pneumococcal conjugate vaccine","volume":"189","author":"Colino","year":"2012","journal-title":"J. Immunol."},{"key":"2025032409400891100_r74","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1093\/intimm\/9.5.689","article-title":"Frequent occurrence of identical heavy and light chain Ig rearrangements","volume":"9","author":"Seidl","year":"1997","journal-title":"Int. Immunol."},{"key":"2025032409400891100_r75","doi-asserted-by":"crossref","first-page":"3643","DOI":"10.4049\/jimmunol.142.10.3643","article-title":"A single VH gene is utilized predominantly in anti-BrMRBC hybridomas derived from purified Ly-1 B cells. Definition of the VH11 family","volume":"142","author":"Hardy","year":"1989","journal-title":"J. Immunol."},{"key":"2025032409400891100_r76","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1084\/jem.169.6.1869","article-title":"Restricted immunoglobulin variable region gene usage by normal Ly-1 (CD5+) B cells that recognize phosphatidyl choline","volume":"169","author":"Mercolino","year":"1989","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r77","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.fgb.2009.09.001","article-title":"Cryptococcus neoformans cryoultramicrotomy and vesicle fractionation reveals an intimate association between membrane lipids and glucuronoxylomannan","volume":"46","author":"Oliveira","year":"2009","journal-title":"Fungal Genet. Biol."},{"key":"2025032409400891100_r78","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1093\/intimm\/dxn122","article-title":"The skewed heavy-chain repertoire in peritoneal B-1 cells is predetermined by the selection via pre-B cell receptor during B cell ontogeny in the fetal liver","volume":"21","author":"Yoshikawa","year":"2009","journal-title":"Int. Immunol."},{"key":"2025032409400891100_r79","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.4049\/jimmunol.0802615","article-title":"Intact bacteria inhibit the induction of humoral immune responses to bacterial-derived and heterologous soluble T cell-dependent antigens","volume":"182","author":"Chattopadhyay","year":"2009","journal-title":"J. Immunol."},{"key":"2025032409400891100_r80","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1128\/EC.00044-09","article-title":"Lipophilic dye staining of Cryptococcus neoformans extracellular vesicles and capsule","volume":"8","author":"Nicola","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"2025032409400891100_r81","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1084\/jem.192.1.87","article-title":"A B cell superantigen-induced persistent \u201cHole\u201d in the B-1 repertoire","volume":"192","author":"Silverman","year":"2000","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r82","doi-asserted-by":"crossref","first-page":"6359","DOI":"10.4049\/jimmunol.0902473","article-title":"Generation of B cell memory to the bacterial polysaccharide \u03b1-1,3 dextran","volume":"183","author":"Foote","year":"2009","journal-title":"J. Immunol."},{"key":"2025032409400891100_r83","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1046\/j.1365-2958.2003.03780.x","article-title":"Cell wall \u03b1-1,3-glucan is required to anchor the Cryptococcus neoformans capsule","volume":"50","author":"Reese","year":"2003","journal-title":"Mol. Microbiol."},{"key":"2025032409400891100_r84","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1111\/j.1365-2958.2006.05551.x","article-title":"Loss of cell wall \u03b1(1-3) glucan affects Cryptococcus neoformans from ultrastructure to virulence","volume":"63","author":"Reese","year":"2007","journal-title":"Mol. Microbiol."},{"key":"2025032409400891100_r85","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1074-7613(00)00006-6","article-title":"Diversity in the CDR3 region of VH is sufficient for most antibody specificities","volume":"13","author":"Xu","year":"2000","journal-title":"Immunity"},{"key":"2025032409400891100_r86","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1084\/jem.20052217","article-title":"Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production","volume":"203","author":"Ippolito","year":"2006","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r87"},{"key":"2025032409400891100_r88","doi-asserted-by":"crossref","first-page":"879","DOI":"10.4049\/jimmunol.1100957","article-title":"Limiting CDR-H3 diversity abrogates the antibody response to the bacterial polysaccharide \u03b1 1\u21923 dextran","volume":"187","author":"Mahmoud","year":"2011","journal-title":"J. Immunol."},{"key":"2025032409400891100_r89","doi-asserted-by":"crossref","first-page":"851","DOI":"10.4049\/jimmunol.0902791","article-title":"Terminal deoxynucleotidyl transferase is required for an optimal response to the polysaccharide \u03b1-1,3 dextran","volume":"184","author":"Mahmoud","year":"2010","journal-title":"J. Immunol."},{"key":"2025032409400891100_r90","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1084\/jem.174.1.151","article-title":"The mouse antibody response to infection with Cryptococcus neoformans: VH and VL usage in polysaccharide binding antibodies","volume":"174","author":"Casadevall","year":"1991","journal-title":"J. Exp. Med."},{"key":"2025032409400891100_r91","doi-asserted-by":"crossref","first-page":"5382","DOI":"10.1073\/pnas.1121631109","article-title":"Antigen-specific antibody responses in B-1a and their relationship to natural immunity","volume":"109","author":"Yang","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025032409400891100_r92","doi-asserted-by":"crossref","first-page":"5388","DOI":"10.1073\/pnas.1121627109","article-title":"Antigen-specific memory in B-1a and its relationship to natural immunity","volume":"109","author":"Yang","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/189\/12\/5820\/61755087\/1201514.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/189\/12\/5820\/61755087\/1201514.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T10:29:47Z","timestamp":1743071387000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/189\/12\/5820\/7999038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":92,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,12,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1201514","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2012,12]]},"published":{"date-parts":[[2012,12]]}}}