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However, transferred TCR chains can pair with endogenous TCR chains, resulting in the formation of mispaired TCR dimers and decreased or unspecific reactivity. TCR gene transfer into hematopoietic stem cells (HSCs) is an alternative to create T cells with desired Ag specificity, because in this case expression of endogenous TCR chains is then less likely owing to allelic exclusion. We generated TCR-transduced T cells from peripheral T cells using the lymphocytic choriomeningitis virus\u2013specific P14 TCR. After transfer of the P14 TCR genes into HSCs and subsequent reconstitution of irradiated mice, TCR-engineered HSC-derived T cells were produced. We then compared the Ag-specific T cell populations with P14 TCR-transgenic T cells for their therapeutic efficiency in three in vivo models. In this study, we demonstrate that TCR-transduced T cells and TCR-engineered HSC-derived T cells are comparable in controlling lymphocytic choriomeningitis virus infection in mice and suppress growth of B16 tumor cells expressing the cognate Ag in a comparable manner.<\/jats:p>","DOI":"10.4049\/jimmunol.1202591","type":"journal-article","created":{"date-parts":[[2013,11,30]],"date-time":"2013-11-30T01:41:55Z","timestamp":1385775715000},"page":"206-213","source":"Crossref","is-referenced-by-count":26,"title":["Immunotherapy with TCR-Redirected T Cells: Comparison of TCR-Transduced and TCR-Engineered Hematopoietic Stem Cell\u2013Derived T Cells"],"prefix":"10.1093","volume":"192","author":[{"given":"Lilian","family":"St\u00e4rck","sequence":"first","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine , 13092 Berlin,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katja","family":"Popp","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Freiburg , 79104 Freiburg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanspeter","family":"Pircher","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Freiburg , 79104 Freiburg,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Uckert","sequence":"additional","affiliation":[{"name":"Max Delbr\u00fcck Center for Molecular Medicine , 13092 Berlin,","place":["Germany"]},{"name":"Institute of Biology, Humboldt University Berlin , 10115 Berlin,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,1,1]]},"reference":[{"key":"2025032404050083600_r1","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1126\/science.1076514","article-title":"Cancer regression and autoimmunity in patients after clonal repopulation with antitumor lymphocytes","volume":"298","author":"Dudley","year":"2002","journal-title":"Science"},{"key":"2025032404050083600_r2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0140-6736(95)91150-2","article-title":"Use of gene-modified virus-specific T lymphocytes to control Epstein-Barr-virus-related lymphoproliferation","volume":"345","author":"Rooney","year":"1995","journal-title":"Lancet"},{"key":"2025032404050083600_r3","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1056\/NEJM199510193331603","article-title":"Reconstitution of cellular immunity against cytomegalovirus in recipients of allogeneic bone marrow by transfer of T-cell clones from the donor","volume":"333","author":"Walter","year":"1995","journal-title":"N. 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