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Because IL-2 induces potent activation of the STAT5 transcription factor, we tested the role of STAT5 in CD8+ memory T cell differentiation and function using a model system in which STAT5 activity is inducibly abrogated upon CD8+ T cell activation. We report that STAT5 activity is broadly important for the expansion and effector function of all effector CTL subsets. After pathogen clearance, STAT5 was required for the survival of effector phenotype memory CTLs during the contraction phase. However, despite its role in supporting full primary CD8+ T cell expansion, and unlike IL-2, STAT5 activity is not required for the development of memory CD8+ T cells capable of robust secondary expansion upon rechallenge. Our findings highlight differential requirements for survival signals between primary and secondary effector CTL, and demonstrate that IL-2\u2013dependent programming of memory CD8+ T cells capable of secondary expansion and secondary effector differentiation is largely STAT5 independent.<\/jats:p>","DOI":"10.4049\/jimmunol.1202674","type":"journal-article","created":{"date-parts":[[2013,2,26]],"date-time":"2013-02-26T03:51:06Z","timestamp":1361850666000},"page":"3390-3398","source":"Crossref","is-referenced-by-count":12,"title":["Disparate Roles for STAT5 in Primary and Secondary CTL Responses"],"prefix":"10.1093","volume":"190","author":[{"given":"Diana M","family":"Mitchell","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Utah , Salt Lake City, UT 84112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Utah , Salt Lake City, UT 84112"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,4,1]]},"reference":[{"key":"2025032406480845900_r1","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.it.2011.01.004","article-title":"Cytokines and the inception of CD8 T cell responses","volume":"32","author":"Cox","year":"2011","journal-title":"Trends Immunol."},{"key":"2025032406480845900_r2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.immuni.2009.11.010","article-title":"Prolonged interleukin-2Ralpha expression on virus-specific CD8+ T cells favors terminal-effector differentiation in vivo","volume":"32","author":"Kalia","year":"2010","journal-title":"Immunity"},{"key":"2025032406480845900_r3","doi-asserted-by":"crossref","first-page":"6719","DOI":"10.4049\/jimmunol.0904089","article-title":"Distinct roles for IL-2 and IL-15 in the differentiation and survival of CD8+ effector and memory T cells","volume":"184","author":"Mitchell","year":"2010","journal-title":"J. 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