{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:02:18Z","timestamp":1790424138721,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The human leukocyte Ag HLA-B27 (B27) is strongly associated with the spondyloarthritides. B27 can be expressed at the cell surface of APC as both classical \u03b22-microglobulin\u2013associated B27 and B27 free H chain forms (FHC), including disulfide-bonded H chain homodimers (termed B272). B27 FHC forms, but not classical B27, bind to KIR3DL2. HLA-A3, which is not associated with spondyloarthritis (SpA), is also a ligand for KIR3DL2. In this study, we show that B272 and B27 FHC bind more strongly to KIR3DL2 than other HLA-class I, including HLA-A3. B272 tetramers bound KIR3DL2-transfected cells more strongly than HLA-A3. KIR3DL2Fc bound to HLA-B27\u2013transfected cells more strongly than to cells transfected with other HLA-class I. KIR3DL2Fc pulled down multimeric, dimeric, and monomeric FHC from HLA-B27\u2013expressing cell lines. Binding to B272 and B27 FHC stimulated greater KIR3DL2 phosphorylation than HLA-A3. B272 and B27 FHC stimulated KIR3DL2CD3\u03b5-transduced T cell IL-2 production to a greater extent than control HLA-class I. KIR3DL2 binding to B27 inhibited NK IFN-\u03b3 secretion and promoted greater survival of KIR3DL2+ CD4 T and NK cells than binding to other HLA-class I. KIR3DL2+ T cells from B27+ SpA patients proliferated more in response to Ag presented by syngeneic APC than the same T cell subset from healthy and disease controls. Our results suggest that expansion of KIR3DL2-expressing leukocytes observed in B27+ SpA may be explained by the stronger interaction of KIR3DL2 with B27 FHC.<\/jats:p>","DOI":"10.4049\/jimmunol.1202926","type":"journal-article","created":{"date-parts":[[2013,2,26]],"date-time":"2013-02-26T03:51:06Z","timestamp":1361850666000},"page":"3216-3224","source":"Crossref","is-referenced-by-count":89,"title":["KIR3DL2 Binds to HLA-B27 Dimers and Free H Chains More Strongly than Other HLA Class I and Promotes the Expansion of T Cells in Ankylosing Spondylitis"],"prefix":"10.1093","volume":"190","author":[{"given":"Isabel","family":"Wong-Baeza","sequence":"first","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Ridley","sequence":"additional","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackie","family":"Shaw","sequence":"additional","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Hatano","sequence":"additional","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliwia","family":"Rysnik","sequence":"additional","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsty","family":"McHugh","sequence":"additional","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Piper","sequence":"additional","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Brackenbridge","sequence":"additional","affiliation":[{"name":"Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine , Headington, Oxford OX3 9DS,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine , Headington, Oxford OX3 9DS,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthoni","family":"Chan","sequence":"additional","affiliation":[{"name":"Royal Berkshire Hospital , Reading RG1 5AN,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Bowness","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Oxford National Institute for Health Research , Oxford OX3 9DU,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Kollnberger","sequence":"additional","affiliation":[{"name":"Nuffield Department of Rheumatological and Musculoskeletal Sciences, Botnar Research Centre , Oxford OX3 7LD,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,4,1]]},"reference":[{"key":"2025032406454168500_r1","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1136\/ard.55.4.268","article-title":"HLA class I associations of ankylosing spondylitis in the white population in the United Kingdom","volume":"55","author":"Brown","year":"1996","journal-title":"Ann. 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