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Adoptive transfer studies suggested that gV-sPLA2 in dendritic cells was necessary for sensitization of Pla2g5-null mice, but was not sufficient to induce the effector phase of pulmonary inflammation. In this study, we demonstrate that gV-sPLA2 is inducibly expressed in mouse and human macrophages (M\u03d5) activated by IL-4 and is required for the acquisition of M\u03d5 effector functions that facilitate the effector phase of pulmonary inflammation. We demonstrate that gV-sPLA2 expression in M\u03d5 is sufficient for the development of pulmonary inflammation, even when inflammation is induced by intrapulmonary administration of IL-4. The concentrations of CCL22\/CCL17 and effector T cell recruitment are severely impaired in Pla2g5-null mice. Intratracheal transfers of enriched CD68+ cells isolated from the lungs of D. farinae\u2013challenged WT donor mice induce eosinophilia, chemokine production, and recruitment of T cells into the lungs of Pla2g5-null recipients previously sensitized by WT D. farinae\u2013loaded dendritic cells. Our studies identified a unique function of gV-sPLA2 in activation of M\u03d5 and in their capacity to recruit T cells to amplify the effector phase of pulmonary inflammation.<\/jats:p>","DOI":"10.4049\/jimmunol.1203202","type":"journal-article","created":{"date-parts":[[2013,5,7]],"date-time":"2013-05-07T03:44:07Z","timestamp":1367898247000},"page":"5927-5938","source":"Crossref","is-referenced-by-count":48,"title":["Group V Secretory Phospholipase A2 Is Involved in Macrophage Activation and Is Sufficient for Macrophage Effector Functions in Allergic Pulmonary Inflammation"],"prefix":"10.1093","volume":"190","author":[{"given":"Shin","family":"Ohta","sequence":"first","affiliation":[{"name":"Department of Medicine, Harvard Medical School , Boston, MA 02115"},{"name":"Department of Pediatrics, Harvard Medical School , Boston, MA 02115"},{"name":"Division of Rheumatology, Immunology and Allergy, Jeff and Penny Vinik Center for Allergic Disease Research, Brigham and Women\u2019s Hospital , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Imamura","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School , Boston, MA 02115"},{"name":"Division of Rheumatology, Immunology and Allergy, Jeff and Penny Vinik Center for Allergic Disease Research, Brigham and Women\u2019s Hospital , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School , Boston, MA 02115"},{"name":"Division of Rheumatology, Immunology and Allergy, Jeff and Penny Vinik Center for Allergic Disease Research, Brigham and Women\u2019s Hospital , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua A","family":"Boyce","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School , Boston, MA 02115"},{"name":"Division of Rheumatology, Immunology and Allergy, Jeff and Penny Vinik Center for Allergic Disease Research, Brigham and Women\u2019s Hospital , Boston, MA 02115"},{"name":"Division of Allergy and Respiratory Medicine, Department of Internal Medicine, School of Medicine, Showa University , Tokyo 142-8555,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Balestrieri","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harvard Medical School , Boston, MA 02115"},{"name":"Division of Rheumatology, Immunology and Allergy, Jeff and Penny Vinik Center for Allergic Disease Research, Brigham and Women\u2019s Hospital , Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,6,15]]},"reference":[{"key":"2025032407080064200_r1","doi-asserted-by":"crossref","first-page":"S237","DOI":"10.1194\/jlr.R800033-JLR200","article-title":"Phospholipase A2 structure\/function, mechanism, and signaling","volume":"50","author":"Burke","year":"2009","journal-title":"J. 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