{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T10:48:00Z","timestamp":1756896480421,"version":"3.40.3"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Invariant NKT (iNKT) cells play key roles in host defense by recognizing lipid Ags presented by CD1d. iNKT cells are activated by bacterial-derived lipids and are also strongly autoreactive toward self-lipids. iNKT cell responsiveness must be regulated to maintain effective host defense while preventing uncontrolled stimulation and potential autoimmunity. CD1d-expressing thymocytes support iNKT cell development, but thymocyte-restricted expression of CD1d gives rise to Ag hyperresponsive iNKT cells. We hypothesized that iNKT cells require functional education by CD1d+ cells other than thymocytes to set their correct responsiveness. In mice that expressed CD1d only on thymocytes, hyperresponsive iNKT cells in the periphery expressed significantly reduced levels of tyrosine phosphatase SHP-1, a negative regulator of TCR signaling. Accordingly, heterozygous SHP-1 mutant mice displaying reduced SHP-1 expression developed a comparable population of Ag hyperresponsive iNKT cells. Restoring nonthymocyte CD1d expression in transgenic mice normalized SHP-1 expression and iNKT cell reactivity. Radiation chimeras revealed that CD1d+ dendritic cells supported iNKT cell upregulation of SHP-1 and decreased responsiveness after thymic emigration. Hence, dendritic cells functionally educate iNKT cells by tuning SHP-1 expression to limit reactivity.<\/jats:p>","DOI":"10.4049\/jimmunol.1203466","type":"journal-article","created":{"date-parts":[[2013,2,21]],"date-time":"2013-02-21T07:02:43Z","timestamp":1361430163000},"page":"3299-3308","source":"Crossref","is-referenced-by-count":11,"title":["Functional Education of Invariant NKT Cells by Dendritic Cell Tuning of SHP-1"],"prefix":"10.1093","volume":"190","author":[{"given":"Anna","family":"Napolitano","sequence":"first","affiliation":[{"name":"Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute , 20132 Milano,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Pittoni","sequence":"additional","affiliation":[{"name":"Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute , 20132 Milano,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Beaudoin","sequence":"additional","affiliation":[{"name":"INSERM U986, H\u00f4pital Saint Vincent de Paul\/Cochin, Universit\u00e9 Paris Descartes , 75014 Paris,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes","family":"Lehuen","sequence":"additional","affiliation":[{"name":"INSERM U986, H\u00f4pital Saint Vincent de Paul\/Cochin, Universit\u00e9 Paris Descartes , 75014 Paris,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Voehringer","sequence":"additional","affiliation":[{"name":"Department of Infection Biology, University Clinic of Erlangen, Friedrich Alexander University , 91054 Erlangen,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H Robson","family":"MacDonald","sequence":"additional","affiliation":[{"name":"Ludwig Center for Cancer Research of the University of Lausanne , 1066 Epalinges,","place":["Switzerland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Dellabona","sequence":"additional","affiliation":[{"name":"Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute , 20132 Milano,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Casorati","sequence":"additional","affiliation":[{"name":"Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute , 20132 Milano,","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,4,1]]},"reference":[{"key":"2025032406475667500_r1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1146\/annurev.immunol.25.022106.141711","article-title":"The biology of NKT cells","volume":"25","author":"Bendelac","year":"2007","journal-title":"Annu. 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